THROMBOLYTIC ACTIVITY OF Punica granatum FRUIT AND PEEL EXTRACT
Abstract
Objective: The present study was undertaken to investigate the thrombolytic activity of Punica granatum fruit and peel extract.
Methods: Phytoconstituents and free radical scavenging activity of P. granatum extract were performed. Ethanolic and aqueous extract of fruit and
peel of the plant was used for in vitro clot lysis activity using streptokinase (SK) as standard. In a series of pre-weighed eppendorf tubes, equal volume
of venous blood was collected and serum was removed and the tubes were weighed. The tubes containing clot were incubated with fruit, peel extract
(100 ug/ml), and SK individually at 37°C for 60 minutes. After incubation, fluid obtained was removed, and tubes were again weighed to observe the
difference in weight after clot disruption. The percent of clot lysis was determined.
Results: Free radical scavenging activity of the extracts was increased in a dose-dependent manner. Of the extracts, ethanolic extract showed
maximum activity as compared with aqueous extract, whereas the ascorbic acid exerted maximum activity as compared with the extracts. Maximum
thrombolytic activity (59%) was observed with ethanolic extract of peel of the fruit.
Conclusions: The present study concluded that the aqueous and ethanolic extracts of P. granatum fruit and peel possess thrombolytic activity.
Keywords: Punica granatum, Antithrombolytic activity, Antibacterial, Antioxidant, Phytochemicals.
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References
Lee HS. How safe is the readministration of streptokinase? Drug Saf
;13(2):76-80.
Collen D. Coronary thrombolysis: Streptokinase or recombinant tissuetype
plasminogen activator? Ann
Intern Med 1990;112(7):529-38.
Rouf SA, Moo-Young M, Chisti Y. Tissue-type plasminogen activator:
Characteristics, applications and production technology. Biotechnol
Adv 1996;14(3):239-66.
Jennings K. Antibodies to streptokinase. BMJ 1996;312(7028):393-4.
Gesler WM. Therapeutic landscapes: Medical issues in light of the new
cultural geography. Soc Sci Med 1992;34(7):735-46.
Kaur G, Jabbar Z, Athar M, Alam MS. Punica granatum (pomegranate)
flower extract possesses potent antioxidant activity and abrogates
Fe-NTA induced hepatotoxicity in mice. Food Chem Toxicol
;44(7):984-93.
Seeram NP, Adams LS, Henning SM, Niu Y, Zhang Y, Nair MG,
et al. In vitro antiproliferative, apoptotic and antioxidant activities
of punicalagin, ellagic acid and a total pomegranate tannin extract
are enhanced in combination with other polyphenols as found in
pomegranate juice. J Nutr Biochem 2005;16(6):360-7.
Mertens-Talcott SU, Jilma-Stohlawetz P, Rios J, Hingorani L,
Derendorf H. Absorption, metabolism, and antioxidant effects of
pomegranate (Punica granatum L.) polyphenols after ingestion of a
standardized extract in healthy human volunteers. J Agric Food Chem
;54(23):8956-61.
Adams LS, Seeram NP, Aggarwal BB, Takada Y, Sand D, Heber D.
Pomegranate juice, total pomegranate ellagitannins, and punicalagin
suppress inflammatory cell signaling in colon cancer cells. J Agric Food
Chem 2006;54(3):980-5.
Harborne JB. Phytochemical Methods. A Guide to Modern Techniques
of Plant Analysis. London: Chapman and Hall; 1984.
Khandelwal KR. Practical Pharmacognosy Techniques and
Experiments. 17
ed. Pune: Nirali Prakashan; 2007.
Gyamfi MA, Aniya Y. Antioxidant properties of Thonningianin A,
th
isolated from the African medicinal herb, Thonningia sanguinea.
Biochem Pharmacol 2002;63(9):1725-37.
Oyaizu M. Studies on product of browning reaction prepared from
glucose amine. Jpn J Nutr 1986;44:307-15.
Ratnasooriya WD, Fernando TS, Madubashini PP. Antioxidant,
antimalarial and antimicrobial activities of tannin-rich fractions,
ellagitannins and phenolic acids from Punica granatum L. Planta Med
;73:352-7.
Blois MS. Antioxidant determinations by the use of a stable free radical.
Nature 1958;181:1199-200.
Collins CH, Lyne PM, Grange JM. Microbiological Methods. 7
ed.,
Vol. 23. Oxford: Butterworth-Heinemann; 1995. p. 178-9.
AlanÃs AD, Calzada F, Cervantes JA, Torres J, Ceballos GM.
Antibacterial properties of some plants used in Mexican
traditional medicine for the treatment of gastrointestinal disorders.
J Ethnopharmacol 2005;100(1-2):153-7.
Liu RH. Flavonoid intake and risk of chronic diseases. Am J Clin Nutr
;78:517-20.
Varadarajan G, Rathinaswamy P, Asirvatahm D. Antimicrobial
properties and phytochemical constituents of Rheo discolor.
Ethnobotanical Lealf 2008;12:841-5.
Reuben KD, Abdulrahman FI, Akan JC, Usman H, Sodipo OA,
Egwu GO. Phytochemical screening and in vitro antimicrobial
investigation of methanolic extract Croton zambesicus. Muell. Arg.
Stem bark. Eur J Sci Res 2008;23:134-40.
Kumar A, Ilavarasan R, Jayachandran T, Decaraman M, Aravindhan P,
th
Padmanabhan N, et al. Phytochemicals investigation on a tropical plant,
Syzygium cumini from Kattuppalayam, Erode District, Tamil Nadu,
South India. Pak J Nutr 2009;8:83-5.
Olaleye MT. Cytotoxicity and antibacterial activity of methanolic
extract of Hibiscus sabdariffa. J Med Plants Res 2007;1:9-13.
Doshi GM, Desai KS, Mukesh DS, Gayatri VA, Preeja GP.
;24:178-9.
Ekop AS. Determination of chemical composition of Gnetum africanum
(AFANG) seeds. Pak J Nutr 2007;6:40-3.
Meir S, Kanner J, Akiri B, Hadar SP. Determination and involvement of
aqueous reducing compounds in oxidative systems of various senscing
leaves Agric Food Chem 1995;43:1813-7.
McLaughlin JL. In assays for bioactivity. In: Hostettmann K, editor.
Methods in Plant Biochemistry. 6
ed., Vol. 34. San Diego, USA:
Academic Press; 1991. p. 132-4.
th
Yamamoto J, Taka T, Yamada K, Ijiri Y, Murakami M, Hirata Y,
et al. Tomatoes have natural anti-thrombotic effects. Br J Nutr
;90:1031-8.
Prasad S, Kashyap SR, Deopujari JY, Purohit HJ, Taori GM,
Daginawala HF. Effect of Fagonia arabica (Dhamasa) on in vitro
thrombolysis. BMC Complement Altern Med 2007;7(36):1-6.
Ozben B, Ekmekci A, Burga Z. Multiple coronary thrombosis and
stent implantation to the subtotally occluded light renal artery in
a patient with essential thrombocytosis. J Thromb Thrombolysis
;22:79-84.
Ali MS, Amin MR, Kamal CM, Hossain MA. In vitro antioxidant,
cytotoxic, thrombolytic activities and phytochemical evaluation of
methanol extract of the A. philippense L. leaves. Asian Pac J Trop
Biomed 2013;3:464-9.
Sivanandham V. Free radicals in health and diseases – A mini review.
J Pharmacologyonline Newsl 2011;1:1062-77.
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