MOLECULAR DETECTION OF HUMAN RHINOVIRUS IN RESPIRATORY SAMPLES OF SWINE FLU NEGATIVE NORTH INDIAN CHILDREN WITH FLU-LIKE ILLNESS

Authors

  • Pooja Gaur Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Neena Srivastava King George’s Medical University, Lucknow
  • Shally Awasthi King George’s Medical University, Lucknow
  • Ravish Katiyar Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Nikky N Shrivastava Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Dharam V Singh Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Shilpa Kaistha Chhatrapati, Shahu, Ji, Maharaj, University
  • Rambha Tripathi Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Virendra K Misra Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Vijay Prakash Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Prerna Kapoor Sanjay Gandhi Post Graduate Institute of Medical Sciences
  • Tapan N Dhole Sanjay Gandhi Post Graduate Institute of Medical Sciences

Abstract

Objectives: Flu-like illness may also be caused by different respiratory viruses other than influenza. Human rhinovirus (HRV) shows almost flu-like
symptoms. The purpose of this study is the molecular detection of HRV in throat swab of swine flu negative North Indian children during the years
2012 and 2013. Reverse transcriptase (RT) - polymerase chain reaction (PCR) amplification of 5'non-coding region (NCR) was used for HRV detection
followed by cell culture isolation of HRV.
Methods: PCR confirmed swine flu negative throat swab samples were collected from the Department of Microbiology, Sanjay Gandhi Post Graduate
Institute of Medical Sciences, Lucknow, Uttar Pradesh, India. The RNA isolation of samples was done using the QIAamp
Viral RNA Mini Kit (Qiagen),
followed by single step RT-PCR amplification (AgPath-ID, Life Technologies). All PCR positive HRV samples were cell cultured in HeLa and HEp-2 cell
lines for viral isolation.
®
Results: 135 swine flu negative throat swab samples were examined. Out of which 34 samples (25.2%) were found HRV positive by RT-PCR, while only
four samples (11.8%) were culture positive on HeLa cell line. Younger children (0-4 year) were found more susceptible to HRV infection. This study
indicated the highest prevalence of HRV (37.0%) during the months (September-October) of the Autumn season in 2012 and 57% in Winter-spring
season (February-March) during 2013.
Conclusion: HRV may be a cause of flu-like symptoms in swine flu suspected North Indian children with a higher rate during Autumn and Spring
season. Molecular detection of HRV using RT-PCR is more sensitive than cell culture assay.
Keywords: Human rhinovirus, Swine flu, Influenza-like illness, Lower respiratory tract infections.

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Author Biographies

Pooja Gaur, Sanjay Gandhi Post Graduate Institute of Medical Sciences

SRF, Department of Microbiology

Neena Srivastava, King George’s Medical University, Lucknow

Professor, Department of Physiology

Shally Awasthi, King George’s Medical University, Lucknow

Professor, Department of Paediatrics

Ravish Katiyar, Sanjay Gandhi Post Graduate Institute of Medical Sciences

SRF, Department of Microbiology

Nikky N Shrivastava, Sanjay Gandhi Post Graduate Institute of Medical Sciences

Department of Microbiology

Dharam V Singh, Sanjay Gandhi Post Graduate Institute of Medical Sciences

SRF, Microbiology

Shilpa Kaistha, Chhatrapati, Shahu, Ji, Maharaj, University

Asst. Professor, Department of Microbiology

Rambha Tripathi, Sanjay Gandhi Post Graduate Institute of Medical Sciences

SRF, Department of Microbiology

Virendra K Misra, Sanjay Gandhi Post Graduate Institute of Medical Sciences

Technician, Department of Microbiology

Vijay Prakash, Sanjay Gandhi Post Graduate Institute of Medical Sciences

Technician, Department of Microbiology

Prerna Kapoor, Sanjay Gandhi Post Graduate Institute of Medical Sciences

Physician, General Hospital

Tapan N Dhole, Sanjay Gandhi Post Graduate Institute of Medical Sciences

Professor and Head, Department of Microbiology

References

Hayden FG. Rhinovirus and the lower respiratory tract. Rev Med Virol

;14(1):17-31.

Bryce J, Boschi-Pinto C, Shibuya K, Black RE; WHO Child Health

Epidemiology Reference Group. WHO estimates of the causes of death

in children. Lancet 2005;365(9465):1147-52.

Rawlinson WD, Waliuzzaman Z, Carter IW, Belessis YC,

Gilbert KM, Morton JR. Asthma exacerbations in children associated

with rhinovirus but not human metapneumovirus infection. J Infect Dis

;187(8):1314-8.

Savolainen-Kopra C, Blomqvist S, Kaijalainen S, Jounio U, Juvonen R,

Peitso A, et al. All known human rhinovirus species are present in

sputum specimens of military recruits during respiratory infection.

Viruses 2009;1(3):1178-89.

Lau SK, Yip CC, Tsoi HW, Lee RA, So LY, Lau YL, et al. Clinical

features and complete genome characterization of a distinct human

rhinovirus (HRV) genetic cluster, probably representing a previously

undetected HRV species, HRV-C, associated with acute respiratory

illness in children. J Clin Microbiol 2007;45(11):3655-64.

Kiang D, Kalra I, Yagi S, Louie JK, Boushey H, Boothby J, et al. Assay

for 5’ noncoding region analysis of all human rhinovirus prototype

strains. J Clin Microbiol 2008;46(11):3736-45.

Loens K, Ieven M, Ursi D, De Laat C, Sillekens P, Oudshoorn P,

et al. Improved detection of rhinoviruses by nucleic acid sequencebased

amplification after

nucleotide sequence

determination of

the 5’

noncoding

regions of additional rhinovirus

strains. J

Clin

Microbiol

;41(5):1971-6.

Blomqvist S, Skyttä A, Roivainen M, Hovi T. Rapid detection of

human rhinoviruses in nasopharyngeal aspirates by a microwell

reverse transcription-PCR-hybridization assay. J Clin Microbiol

;37(9):2813-6.

Steininger C, Aberle SW, Popow-Kraupp T. Early detection of acute

rhinovirus infections by a rapid reverse transcription-PCR assay. J Clin

Microbiol 2001;39(1):129-33.

Lee WM, Kiesner C, Pappas T, Lee I, Grindle K, Jartti T, et al. A diverse

group of previously unrecognized human rhinoviruses are common

causes of respiratory illnesses in infants. PLoS One 2007;2(10):e966.

Bizzintino J, Lee WM, Laing IA, Vang F, Pappas T, Zhang G, et al.

Association between human rhinovirus C and severity of acute asthma

in children. Eur Respir J 2011;37(5):1037-42.

Khetsuriani N, Lu X, Teague WG, Kazerouni N, Anderson LJ,

Erdman DD. Novel human rhinoviruses and exacerbation of asthma in

children. Emerg Infect Dis 2008;14(11):1793-6.

Brownlee JW, Turner RB. New developments in the epidemiology

and clinical spectrum of rhinovirus infections. Curr Opin Pediatr

;20(1):67-71.

Miller EK, Lu X, Erdman DD, Poehling KA, Zhu Y, Griffin MR, et al.

Rhinovirus-associated hospitalizations in young children. J Infect Dis

;195(6):773-81.

Savolainen C, Blomqvist S, Hovi T. Human rhinoviruses. Paediatr

Respir Rev 2003;4(2):91-8.

Lu X, Holloway B, Dare RK, Kuypers J, Yagi S, Williams JV, et al.

Real-time reverse transcription-PCR assay for comprehensive detection

of human rhinoviruses. J Clin Microbiol 2008;46(2):533-9.

Loens K, Goossens H, de Laat C, Foolen H, Oudshoorn P, Pattyn S,

et al. Detection of rhinoviruses by tissue culture and two independent

amplification techniques, nucleic acid sequence-based amplification

and reverse transcription-PCR, in children with acute respiratory

infections during a winter season. J Clin Microbiol 2006;44(1):166-71.

Huang T, Wang W, Bessaud M, Ren P, Sheng J, Yan H, et al. Evidence

of recombination and genetic diversity in human rhinoviruses in

children with acute respiratory infection. PLoS One 2009;4(7):e6355.

Renwick N, Schweiger B, Kapoor V, Liu Z, Villari J, Bullmann R,

et al. A recently identified rhinovirus genotype is associated with

severe respiratory-tract infection in children in Germany. J Infect Dis

;196(12):1754-60.

Amineva SP, Aminev AG, Gern JE, Palmenberg AC. Comparison of

rhinovirus A infection in human primary epithelial and HeLa cells.

J Gen Virol 2011;92:2549-57.

Mackay IM. Human rhinoviruses: The cold wars resume. J Clin Virol

;42(4):297-320.

Arruda E, Crump CE, Rollins BS, Ohlin A, Hayden FG. Comparative

susceptibilities of human embryonic fibroblasts and HeLa cells for

isolation of human rhinoviruses. J Clin Microbiol 1996;34(5):1277-9.

Smuts HE, Workman LJ, Zar HJ. Human rhinovirus infection in young

African children with acute wheezing. BMC Infect Dis 2011;11:65.

Arruda E, Pitkäranta A, Witek TJ Jr, Doyle CA, Hayden FG. Frequency

and natural history of rhinovirus infections in adults during autumn.

J Clin Microbiol 1997;35(11):2864-8.

Zhao LQ, Qian Y, Zhu RN, Deng J, Wang F. Study on the status of

human rhinovirus infections in infants and young children with acute

respiratory infections in Beijing, from 2002 to 2006. Zhonghua Liu

Xing Bing Xue Za Zhi 2007;28(7):683-5.

Published

01-01-2016

How to Cite

Gaur, P., N. Srivastava, S. Awasthi, R. Katiyar, N. N. Shrivastava, D. V. Singh, S. Kaistha, R. Tripathi, V. K. Misra, V. Prakash, P. Kapoor, and T. N. Dhole. “MOLECULAR DETECTION OF HUMAN RHINOVIRUS IN RESPIRATORY SAMPLES OF SWINE FLU NEGATIVE NORTH INDIAN CHILDREN WITH FLU-LIKE ILLNESS”. Asian Journal of Pharmaceutical and Clinical Research, vol. 9, no. 1, Jan. 2016, pp. 272-4, https://journals.innovareacademics.in/index.php/ajpcr/article/view/8171.

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