PHARMACOLOGICAL EVALUATION OF RANGER SYRUP BY STUDYING ACUTE TOXICITY, IN-VITRO ANTI-OXIDANT PROPERTY AND IN-VIVO ANTI-STRESS ACTIVITY
Keywords:
Ranger Syrup, acute toxicity, in-vivo anti-stress activity, in-vitro anti-oxidant propertyAbstract
Stress and stress-related disorders are a significant cause of disease in modern times, perhaps contributing to 75% of illnesses. It has been reported that exposure to physical and mental stress situations can stimulate numerous pathways, leading to increased production of oxygen free radicals. In such situation, the antioxidant defense systems of body become unable to scavenge these free radicals sufficiently. Ranger Syrup is a polyherbal Ayurvedic medicine which was taken up in the present study to evaluate acute toxicity, in-vitro anti-oxidant property and in-vivo anti-stress activity. Acute toxicity study was carried out on wistar rats as per OECD Guidelines 423. To evaluate anti-stress activity, forced swimming endurance test was performed. During this study, difference in rectal temperature and swimming time was noted. Blood samples were collected for estimation of serum cortisol and serum glucose level. Anti-oxidant property of Ranger Syrup was determined by the in-vitro ferric reducing anti-oxidant power (FRAP) assay at various concentration. Study data revealed that Ranger syrup didn't produced any toxic effect even up to the dose level of 5000mg/kg hence was proven as safe. The pre-treatment of Ranger Syrup prevented the fall of rectal temperature in highly significant manner and increased swimming time in comparison to stress control group. Ranger Syrup treated animals showed significant restriction in elevation of serum cortisol and serum glucose level as compared to stress control group. In addition, it has significant potential to scavenge free radicals. From the results of these studies, it can be concluded that Ranger Syrup is safe, capable to increase tolerance against non-specific stress in experimental animals and having significant anti-oxidant activity.
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