EFFECT OF SPROUTING ON IN-VITRO ANTIOXIDANT POTENTIAL OF SOME VARIETIES OF CHICKPEA SEEDS (CICER ARIETINUM LINN.)

Authors

  • Jasneet Kaur ASBASJSM College of Pharmacy
  • Satnam Singh ASBASJSM College of Pharmacy, Bela, Ropar

Abstract

 Objective: The aim of the present study is to access the effect of sprouting on in-vitro antioxidant potential of some varieties of Cicer aurantium Linn. (Chickpea seeds).

Materials and Methods: The un-sprouted and sprouted seeds of its newly developed varieties viz. PBG-1, GPF-2, PBG-5 were powdered and then was extracted with methanol by direct maceration method and their antioxidant activity was evaluated using four different methods (Reducing power, Free radical scavenging activity (DPPH), total antioxidant activity and ferric reducing antioxidant power activity).

 Results: Sprouting clearly increased the flavonoid content of seeds to 104%, 117%, 341% respectively. There was visible increase in total antioxidant activity of methanol extracts of sprouted seeds which was evident by increased activity from 0.298 to 0.397 at concentration 60 µg/ml in var. PBG-5 and increased ferric reducing antioxidant power from 0.332 to 0.387 at concentration 40 µg/ml in var. PBG-1.

 Conclusion: Thus it may be concluded from the study that sprouting visibly increased the in-vitro antioxidant activity of selected chickpea seeds varieties.

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Author Biographies

Jasneet Kaur, ASBASJSM College of Pharmacy

M.Pharm,Pharmacognosy

Satnam Singh, ASBASJSM College of Pharmacy, Bela, Ropar

Dr. Satnam Singh, Assoc. Professor and Head Dept of Pharmacognosy, ASBASJSM College of Pharmacy, Bela, Ropar, Punjab-140111.

References

Yafang S, Gan Z., Jinsong B. Total phenolic content and antioxidant capacity of rice grains with extremely small size. Afr J Agric Res 2011; 6(10): 2289-2293.

Kan A, Ozcelik B, Kartal M. In-vitro antimicrobial activities of Cicer arietinum Linn. (Chickpea), Trop J of Pharma res 2010; 9(5): 475-481.

Segev A, Badani H, Kapulnik Y, Shomer, I, Galili S. Determination of Polyphenols, Flavanoids, and Antioxidant capacity in Colored Chickea (Cicer arietinum Linn.). J food sci 2010; 45: 287-306.

Vadnere GP, Patil AV, Wagh,SS, Jain SK. In–vitro Free Radical Scavenging and Antioxidant activity of Cicer arietinum L. (Fabaceae). International J Pharma Tech Res 2012; 4(1): 343-350.

Dalal K, Singhroha S, Ahlawat S, Patra A. Anti-diarrhoeal activity of roots of Cicer arietinum Linn, Int. J. Res. Pharm. Bio Sci. 2011; 2(1): 268-270.

Dolopodi S, Sandya L, Reddy KC, Nagarjuna S, Reddy P, Shafeen S. Anti-inflammatory activity of Cicer arietinum Linn. seed extracts. Asian J Pharm Clin Res 2012; 5(1): 64-68.

Rehman, K et al. Studies on free radicals, antioxidants, and co-factors. Clin Intervention Aging 2012; 2(2): 219-236.

Rao AS, Reddy SG, Babu PP, Reddy AR. The antioxidant and antiproliferative activities of methanolic extracts from Njavara rice bran. BMC Complement Altern Med 2010, 10(4):1-9.

Tiwari AK, Swapna M, Ayesha SB, Zehra A, Agawane SB, Madhusudana,K. Identification of proglycemic and antihyperglycemicactivity in antioxidant rich fraction of some common food grains. Int Food Res J 2011; 18: 915-923.

Joshi A, Singh N, Pathak AK, Tailang MP. Phytochemistry and evaluation of whole plant of Calotropis gigantean Linn., International J Res Ayurveda and Pharmacy 2010; 1: 120-125.

Alhakmani F, Kumar S, Khan SA. Estimation of total phenolic content, in–vitro antioxidant and anti–inflammatory activity of flowers of Moringa oleifera. Asian Pac J Trop Biomed, 2013, 3(8): 623-32.

Saha MR, Alam A, Akter R, Jahangir R. In-vitro free radical scavenging activity of Ixora coccinea L. Bangladesh J Phamacol 2008; 3: 90-96.

Wang M, Xu YY, G Xu-Dan. Comparison of Antioxidant Activity between Six Kinds of Minor Cereals and Two Kinds of Staple Food Grains [J]. Food Sci 2012; 33(7): 20-25.

Rao BR, Kumar H, Ravikiran M. The ferric reducing ability of plasma (FRAP) as a measure of antioxidant power†the FRAP assay. J Pharm Bioallied Sci 2011, 3(2): 277–279.

Published

01-11-2015

How to Cite

Kaur, J., and S. Singh. “EFFECT OF SPROUTING ON IN-VITRO ANTIOXIDANT POTENTIAL OF SOME VARIETIES OF CHICKPEA SEEDS (CICER ARIETINUM LINN.)”. Asian Journal of Pharmaceutical and Clinical Research, vol. 8, no. 6, Nov. 2015, pp. 265-8, https://journals.innovareacademics.in/index.php/ajpcr/article/view/8529.

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