ANTIPROLIFERATIVE EFFECT OF ETHANOLIC LEAF EXTRACT OF SOLANUM TRILOBATUM ON HEP2 CANCER CELL LINES.

Abstract

Objective: To analyze the antioxidant and antiproliferative potential of Solanum trilobatum was evaluated.

Methods: Free radical scavenging potential of the plant was studied by various in vitro assays such as DPPH, ßCarotene and Frap assay. The antiproliferative activity of Solanum trilobatum was analysed by MTT assay.

Result: The ethanol extract is more powerful in scavenging free radicals. The preliminary phytochemical screening of Solanum trilobatum revealed the presence of phenolics, carbohydrates alkaloids, flavonoids and tannins in high amount. The antiproliferative potential of the ethanol extract was studied on Hep2 cell lines by MTT assay. The extract had an IC50 value of100μg/mL which showed cell viability of 22.67%. From the result, it is clear that ethanol extract of Solanum trilobatum has cytotoxic effect on Hep2 cell lines.

Conclusion: Thus, the study revealed that Solanum trilobatum could be considered as a significant source of antioxidant and antiproliferative agents.

Keywords: Antioxidant, antiproliferative, Solanum trilobtum, DPPH assay, Hep2 cell line.