FORMULATION, CHARACTERISATION AND OPTIMISATION OF NATURAL ARGAN OIL MICROEMULSION FOR TOPICAL DELIVERY

HIND OUHADDOUCH; ZINEB ALIAT; ABDELKADER  LAATIRIS; NAWAL CHERKAOUI; AICHA  FAHRY; YOUNES RAHALI; YASSIR EL ALAOUI

doi:10.22159/ijap.2025v17i1.48514

Authors

HIND OUHADDOUCH Laboratory of Pharmacognosy, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco https://orcid.org/0000-0001-6094-9611
ZINEB ALIAT Laboratory of pharmaceutics, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco
ABDELKADER LAATIRIS Laboratory of pharmaceutics, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco
NAWAL CHERKAOUI Laboratory of pharmaceutics, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco
AICHA FAHRY Laboratory of pharmaceutics, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco
YOUNES RAHALI Laboratory of pharmaceutics, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco
YASSIR EL ALAOUI Laboratory of pharmaceutics, Faculty of Medicine and Pharmacy, Mohammed V University, Rabat, Morocco https://orcid.org/0000-0001-8925-2690

DOI:

https://doi.org/10.22159/ijap.2025v17i1.48514

Keywords:

Microemulsion, Argan oil, surfactant, Cosurfactant

Abstract

Objective: The purpose of this study was to develop, optimize and characterize a stable microemulsion of Moroccan cosmetic Argan oil.

Methods: In this work, microemulsion system was studied by construction of phase diagrams using titration method. At first various surfactants (Brij56®, Tween 80®, Solutol®, Tween 20® and Labrasol®) and various weight ratio surfactant/cosurfactant (1:0, 3:1, 2:1 and 1:1) were tested to select the optimal surfactant and concentration to use. The microemulsions with tween80 were evaluated with different techniques using various parameters such as droplet size, transmittance, viscosity and pH. Stability studies of these microemulsions were conducted for 8 weeks at 5°C, 25°C and 40°C, and underwent centrifugation at 3000 rpm and ultracentrifugation at 10 000 rpm.

Results: The largest microemulsion formation area was achieved for the microemulsions containing Tween 80/PEG 400 at a ratio of 3:1. The obtained microemulsions M1 to M12 were homogeneous. More the percentage of PEG 400 increases, more the pH of the preparations and their viscosity decreases, while preparations with a high oil content have low transmittance. Thermodynamic and physical stability shows that only samples with a minimum of 31.5% of Tween 80 and a maximum of 9% of oil showed a good stability.

Among the stable preparations, M11 (9% O, 10% W and 40.5/40.5% S/Cos) was the formula which exhibited properties such as transparency, soft acidic pH and low viscosity, making it suitable for cutaneous use.

Conclusion: The use of pseudo-ternary phase diagrams allows for the development of an optimal microemulsion with perfect stability.

Keywords: Microemulsion, Argan oil, surfactant, cosurfactant.

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References

Monfalouti HE, Guillaume D, Denhez C, Charrouf Z. Therapeutic potential of Argan oil: a review. J Pharm Pharmacol. 2010 Dec;62(12):1669-75.

Charrouf Z, Wieruszeski JM, Fkih-Tetouani S, Leroy Y, Charrouf M, Fournet B. Triterpenoid saponins from Argania spinosa. Phytochemistry. 1992;31(6):2079-86.

Amzal H, Alaoui K, Tok S, Errachidi A, Charof R, Cherrah Y, Benjouad A. Protective effect of saponins from Argania spinosa against free radical-induced oxidative haemolysis. Fitoterapia. 2008;79(5):337-44.

Guillaume D, Charrouf Z. Argan oil and other Argan products: Use in dermocosmetology. Eur J Lipid Sci Technol. 2011;113(4):403-8.

Tenjarla S. Microemulsions: An overview and pharmaceutical applications. Crit Rev Ther Drug Carrier Syst. 1999;16(5):461-521.

Gupta S, Moulik SP. Biocompatible microemulsions and their prospective uses in drug delivery. J Pharm Sci. 2008;97(1):22-45.

Attivi D, Ajana I, Astier A, Demoré B, Gibaud S. Development of microemulsion of mitotane for improvement of oral bioavailability. Drug Dev Ind Pharm. 2010 Apr;36(4):421-7.

Jalajakshi MN, Chandrakala V, Srinivasan S. An overview : recent development in transdermal drug delivery. Int J Pharm Pharm Sci. 2022;14(10):1-9.

Tole K, Deshmukh G. Design and characterization of microemulsion gel for transdermal drug delivery system of duloxetine hydrochloride. Asian J Pharm Clin Res. 2018;11:157-61.

Kweon JH, Chi SC, Park ES. Transdermal delivery of diclofenac using microemulsions. Arch Pharmacal Res. 2004 Mar;27(3):351-6.

Muzaffar F, Singh UK, Chauhan L. Review on microemulsion as futuristic drug delivery. Int J Pharm Pharm Sci. 2013;5(3):39-53.

Osborne DW, Ward AJ, O'Neill KJ. Microemulsions as topical drug delivery vehicles: in-vitro transdermal studies of a model hydrophilic drug. J Pharm Pharmacol. 1991 Jun;43(6):450-4.

El alaoui Y, Fahry A, Rahali Y, Cherkaoui N, Bensouda Y, Laatiris A. Formulation, optimization and characterization of ibuprofen loaded microemulsion system using d-optimal mixture design. Int J Appl Pharm. 2019 Mar 14;11(4):304-12.

Pessoa RS, França EL, Ribeiro EB, Lanes PK, Chaud NG, Moraes LC, Honorio-França AC. Microemulsion of babassu oil as a natural product to improve human immune system function. Drug Des Dev Ther. 2014 Dec 16;9:21-31.

Bergenholtz J, Romagnoli AA, Wagner NJ. Viscosity, microstructure and interparticle potential of AOT/H2O/n-decane inverse microemulsions. Langmuir. 1995 Feb 21;11:1559–70.

Mehta SK, Dewan RK, Bala K. Percolation phenomenon and the study of conductivity, viscosity, and ultrasonic velocity in microemulsions. Phys Rev E Stat Phys Plasmas Fluids Relat Interdiscip Top. 1994 Dec;50(6):4759-62.

Sharma PK, Kumar A. Development, characterization, and evaluation of celecoxib microemulsion for topical delivery. J Dispersion Sci Technol. 2012;33(10):1411-8.

Gibaud S, Attivi D. Microemulsions for oral administration and their therapeutic applications. Expert Opin Drug Delivery. 2012 Aug;9(8):937-51.

Wang X, Xue M, Gu J, Fang X, Sha X. Transdermal microemulsion drug delivery system for impairing male reproductive toxicity and enhancing efficacy of Tripterygium Wilfordii Hook f. Fitoterapia. 2012 Jun;83(4):690-8.

Xi J, Chang Q, Chan CK, Meng ZY, Wang GN, Sun JB, Wang YT, Tong HH, Zheng Y. Formulation development and bioavailability evaluation of a self-nanoemulsified drug delivery system of oleanolic acid. AAPS Pharm Sci Tech. 2009;10(1):172-82.

Patel V, Kukadiya H, Mashru R, Surti N, Mandal S. Development of microemulsion for solubility enhancement of clopidogrel. Iran J Pharm Res. 2010;9(4):327-34.

Hayakawa F, Kazami Y, Ishihara S, Nakao S, Nakauma M, Funami T, Nichinari K, Kohyama K. Characterization of eating difficulty by sensory evaluation of hydrocolloid gels. Food Hydrocolloids. 2014 jul;38:95-103.

Kumar N, Shishu. D-optimal experimental approach for designing topical microemulsion of itraconazole: Characterization and evaluation of antifungal efficacy against a standardized Tinea pedis infection model in Wistar rats. Eur J Pharm Sci. 2015 Jan 25;67:97-112.

Tadros TF. Emulsion Formation and Stability. 1first edition. Weinheim: Wile-VCH Verlag GmbH & Co; 2013.

Tadros TF. Emulsions Formation, Stability, Industrial Applications. Berlin, Boston: De Gruyter; 2016.

Shafiq S, Shakeel F, Talegaonkar S, Ahmad FJ, Khar RK, Ali M. 2007. Development and bioavailability assessment of ramipril nanoemulsion formulation. Eur J Pharm Biopharm. 2007 may;66:227–43.

Laraib J, Umer Jan S, Gul R. Formulation of microemulsion based gel of salbutamol sulphate and it’s in vitro studies. Int J Curr Pharm Res. 2020 Jul;12(4):102-7.

Santos P, Watkinson AC, Hadgraft J, Lane ME. Application of microemulsions in dermal and transdermal drug delivery. Skin Pharmacol Physiol. 2008;21(5):246-59.

Azeem A, Khan ZI, Aqil M, Ahmad FJ, Khar RK, Talegaonkar S. Microemulsions as a surrogate carrier for dermal drug delivery. Drug Dev Ind Pharm. 2009 May;35(5):525-47.

Lawrence MJ, Rees GD. Microemulsion-based media as novel drug delivery systems. Adv Drug Delivery Rev. 2000 Dec 6;45(1):89-121.

Jadhav AJ, Kadam VJ, Kulkarni SA. Cosurfactants in Microemulsion: A review. J Dispersion Sci Technol. 2020;41(11):1633-52.

Biruss B, Valenta C. The advantage of polymer addition to a non-ionic oil in water microemulsion for the dermal delivery of progesterone. Int J Pharm. 2008 Feb 12;349(1-2):269-73.

Pereira-Lachataignerais J, Pons R, Panizza P, Courbin L, Rouch J, López O. Study and formation of vesicle systems with low polydispersity index by ultrasound method. Chem Phys Lipids. 2006 Apr;140(1-2):88-97.

Yati K, Srifiana Y, Putra F. Effect of optimization of Tween 80 and propylene glycol as a surfactant and cosurfactant on the physical properties of aspirin microemulsion. Int J Appl Pharm. 2017 oct;9(1):127-9.

Pandey RP. Effect of pH and temperature on the hydrolysis of Tween 80. Indian J Biochem Biophys. 2010;47(3):154-8.

Berrada Z, Sabir H, Haddioui F, kihal H. Chemical changes and oxidative stability of Argan oil during heating. Int J Food Sci Technol. 2017;52(4):1074-80.

Formariz TP, Urban MC, Silva AA Jr, Gremião MP, Oliveira AG. Microemulsion and liquid crystals as drug delivery systems. Revista Brasileira de Ciências Farmacêuticas. 2005;41:301–13.

You X, Xing Q, Tuo J, Song W, Zeng Y, Hu H. Optimizing surfactant content to improve oral bioavailability of ibuprofen in microemulsions: just enough or more than enough?. Int J Pharm. 2014 Aug 25;471(1-2):276-84

Chen Y, Tuo J, Huang H, Liu D, You X, Mai J, Song J, Xie Y, Wu C, Hu H. Optimized mixed oils remarkably reduce the amount of surfactants in microemulsions without affecting oral bioavailability of ibuprofen by simultaneously enlarging microemulsion areas and enhancing drug solubility. Int J Pharm. 2015 Jun 20;487(1-2):17-24.

Cichewicz A, Pacleb C, Connors A, Hass MA, Lopes LB. Cutaneous delivery of α-tocopherol and lipoic acid using microemulsions: influence of composition and charge. J Pharm Pharmacol. 2013 Jun;65(6):817-26.