A STUDY OF PHARMACOKINETIC INTERACTION BETWEEN MANGIFERIN AND ATORVASTATIN IN HYPERLIPIDEMIC RATS
DOI:
https://doi.org/10.22159/ijap.2025v17i1.52248Keywords:
Atorvastatin, Mangiferin, Hyperlipidemia, PharmacokineticsAbstract
Objective: The main aim of this study is to determine the effect of Mangiferin on the pharmacokinetics of Atorvastatin in hyperlipidemic rats.
Methods: The plasma concentration of Atorvastatin (ATR) to the time profile was calculated using High Performance Liquid Chromatography (HPLC). The experiment was carried out in normal and High Fat Diet (HFD) induced hyperlipidemic rats in single and multiple-dose treated rats for pharmacokinetic study. HFD was administered to the rats for 8 weeks to induce hyperlipidemia. Each group contains six animals, Group I received ATR alone (20mg/kg), Group II received Mangiferin(MGF) (40mg/kg) followed by ATR for Single-Dose Interaction(SDI), Group III received MGF for 7 days, followed by ATR on 8th day for Multiple Dose Interaction (MDI). Blood samples were collected and estimated for pharmacokinetic parameters such as the Area Under Curve (AUC), Maximum Plasma Concentration (Cmax), Time to achieve Cmax (Tmax), Biological Half-Life(t1/2), Mean Residence Time(MRT) and Volume of distribution(Vd), Clearance (CL) and Elimination Rate Constant (Ke).
Results: In normal and disease-induced rats of SDI and MDI studies, it has shown increased plasma concentration of ATR in the presence of MGF. MGF significantly increased the following pharmacokinetic parameters: Cmax (83.56±5.94 ng/ml) in SDI, (100.28±6.37 ng/ml) in MDI, AUC (265.25±14.73ng.h/ml) in SDI, (299.04±14.11ng.h/ml) in MDI. t1/2, Vd, and MRT were also enhanced in both studies. However, CL and Ke were reduced significantly.
Conclusion: This study revealed that MGF altered the ATR pharmacokinetics. This could be due to MGF Cytochrome P450 (CYP3A) enzyme inhibition.
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