STUDY ON PHYTO-CHEMICAL AND ANTIMICROBIAL ACTIVITY OF SOME SELECTED MARINE SEAWEEDS AGAINST HUMAN AND FISH PATHOGENS
Abstract
Abstract
Seaweeds are used in pharmaceutical and biochemical applications as they possess interesting biological activities that contribute to the discovery of natural therapeutic agents. In the present work, we used four seaweeds (Sargassum wightii, Ulva fasciata, cauterpa racemosa    and Padina gymnospora), extracted in five solvents (chloroform, methanol, petroleum ether, acetone and butanol) and tested for their antimicrobialactivty against 7 human bacterial pathogens (Staphylococcus aureus, Streptococcus mutants, Bacillus subtilus, Pseudomonas aeruginosa, Klebsiella pneumoniae, E.coli and Salmonella typhimurium and 6 fish bacterial pathogens (Aeromonas sp , Pseudomonas sp, Flavobacterium sp, Yersinia sp, and  Vibrio cholera). All the extracts in all the seaweeds were highly effective against bacteria. All the extracted seaweeds contain tannin, phenol, saponins, alkaloids and flavanoids phytochemical compounds. In future, the research may help to preparation of bioactive nanopartical compounds.
Keywords: Marine algae, Human and fish bacterial pathogens, solvents, antibacterial activity
References
References.
Ito,K and Hori,K.. Seaweed: Chemical composition and potential uses. Food Review International. 1989. 5:101-144.
Oh, K.B., Lee, J.H., chang, S.C., Shin, J., Shin, H.J., Kim, H.K.. Antimicrobial activity of the bromophenols from red alga Odonthalia corymbifera and some synthetic derivatives. Bioorganic and Med Chem Lett. 2008. 18:104-108.
Somepalli Venkateswarlu Gopala, K., Panchanula Aditya, I., Gottumukkala Gottumukkala. Subburajau, V.. Synthesis, structural revision and biological activities of 4-chloroaurone, a metabolite of marine brown alga Spatoglossum variabile. Tetrahedron. 2007. 63(29): 6909-6914.
Yang,R.Y., Li,C.Y., Peng,G.T., She,Z.G., Zhou,S.N.. Lactones from a brown alga endophytic fungus (No.ZZF36) from the south china sea and their antimicrobialactivities. Bioorg and Med Chem Lett. 2006. 16(16):4205-4208.
Filton, J.H. Antiviral properties of marine algae. In: Critchly. A.T., Ohno, M., Largo, D.B. World seaweed resources. Workingham, UK: Windows and Macintosch. ETI information services.2006. pp-7
EI-Baroty, G.S., Moussa, M.Y., Shallan. M.A., Ali, M.A., Sabh, A.Z., Shalaby, E.A..Contribution to the aroma biological activities, minerals, protein, pigments, and lipid contents of the red alga: Asparagopsis taxiformis (Dalile) Trevisan.J.Appl.Sci.Rev. 2007. 3.1825-1834.
Al-Fadhli,A., Wahidulla,S., D’Souza,L.. Glycolipids from the red alga Chondria aramta (Kutz).Okamura. Glycobiol. 2006. 6: 902-915.
Kandhasamy,M. and Arunachalam,K.D.. Evaluation of in vitro antimicrobial property of seaweed of Southwest coast of India. African J.Biotech. 2008. 7(12):1958-1961.
Blunt,J.W., Copp,B.R., Hu,W.P., Munro,M.H.G., Northeote,P.T. and Prinsep,M.R.. Marine natural products. Nat.Prod Rep. 2007. 24:31-86.
Dhargalkar, V.K., Jagtap, T.J. and Untawale,A.G.. Biochemical constituents of seaweeds along the Maharashtra coast. Indian J. Marine Sci. 1980. 9(4): 297-299.
Reeta Jayasankar, J. Ramalingam, R. and Kaliaperumal,N.. Biochemical composition of some green algae from Mandapam coast. Seaweed Research Utilisation. 1990. 12 (1&2): 37-40.
Kaliaperumal, N., Chennubhotla ,V.S.K., Najmuddin, M., Ramalingam J.R. and Kalimutthu, S.. Biochemical composition of some common seaweeds from Lakshadweep. J. Mar. Biol. Ass. India. 1994.36(1&2): 316-319.
Burtin, P.. Nutritional Value of Seaweeds, Electronic journal of Environmental, Agricultural and Food chemistry. 2003.2(4): 498-503.
Darcy-Vrillon, B.. Nutritional aspects of the developing use of marine macro algae for the human food industry. International Journal of Food Science and Nutrition . 1993. 44: 23– 35.
Harborne, J.B.Phyto-chemical methods. London: Chapman and Hall;1998.
Engel,S.,Puglisi,M.P., Jensen,P.R. and Fenical,W..Antimicrobial activities of extracts from tropical Atlantic marine plants against marine pathogens and saprophytes. Mar.Bio. 2006. 149:991-1002.
Kolodziej,H., Kiderlen,A.F..Antileishmanial activity and immune modulatory effects oftannins and related compounds of leishmania parasitized RAW 264.7 cells.Phytochem. 2005. 66(17):2056-2071.
George.F., Zohar kerem, Harinder,P.S.M., Klaus Becker.. The biological action of sapanins in animal systems:a review.Brit. J.Nutr. 2002. 88(6):587-605.
Xu,R., Zhao,W., Shao,B.,Qin,G.. Studies on bioactive saponins from Chinese medicinal plants. Adv.Exp.Med and Bio. 1996. 404:371-382.
Cushnie ,T.P.T., Lamb,A.J..Antimicrobial activity of flavonoids. Int.J.Antimicrob.Agents. 2005. 26(5):343-356.
De Sousa.R.R., Queiroz.K.C., Souza,A.C., Gurgueira,S.A., Augusto,A.C .and Miranda,M.A.. Phosphoprotein levels, MAPK activities and NFkappaB expression are affected y fisetin. I.Enzyme Inhib med Chem. 2007. 22(4):439-444.
Kannapiran,E and Nithyanandan,M..Antibacterial activity of different fractions of extracts from Palk Bay Seaweeds Research and Utilization. 2002. 24(1):177-181.
Padmini Sreenivasa Rao,P.. Biological investigation of Indian marine algae and screening of some green, red and brown seaweeds for their antimicrobial activity. Seaweeds Research and Utilization. 1991. 14(10):37-43.
Caccamese,S., Toscanam,R.M., Funari,G and Cormaci,M..Antimicrobial activities of some marine algae from southern Italy coast. Botanica Marina. 1985. 24:505-507.
Schwarz,S. and Noble,W.C.. Aspects of bacterial resistance to antimicrobials used in veterinary dermatological practice. Veterinary Dermatol. 1999. 10:163-176.
Reichelt,J.L. and Borowitzka,M.A.. Antimicrobial activity from algae: Results of large scale screening programme. Hydrobiol. 1984. 116/117:158-168.
Johnsi christobel,G., Lipton,A.P., Aishwarya,M.S., Sarika,A.R.and Udayakumar,A.. Antimicrobial activity of aqueous extract from selected macroalgae of southwest coast of India. Seaweeds Res. Utilization. 2011. 33(1&2):67-75.