PRELIMINARY PHYTOCHEMICAL, PHYSICOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF CLEMATIS MONTANA LEAVES

Authors

  • Hemlata Bhatt Department of Pharmaceutical Sciences, H. N. B. Garhwal University, Srinagar 246174, Garhwal, Uttarakhand
  • Sarla Saklani Department of Pharmaceutical Sciences, H. N. B. Garhwal University, Srinagar 246174
  • Kumud Upadhayay Department of Pharmaceutical Sciences, Bhimtal, Kumaun University, Uttarakhand

DOI:

https://doi.org/10.22159/ijcpr.2016v8i4.15274

Keywords:

Clematis montana, Antibacterial, Antioxidant, Ethanolic extract

Abstract

Objective: To evaluate the phytochemical, physicochemical, antimicrobial and in-vitro antioxidant activity of Clamentis montana (Family: Rananculaceae).

Methods: The extracts were evaluated for antibacterial activity against S. aureus, B. subtilis, E. coli, P. aeruginosa by cup plate method. In-vitro antioxidant activity was done by DPPH, ferrous chelating and reducing power assay method. The physicochemical parameter like a loss on drying, total ash value, foreign matter, etc. was evaluated by standard protocol.

Results: The extract showed significant antibacterial activity against all test strains when compared with standard drugs amoxicillin. The extract showed significant antioxidant activity by DPPH method, reducing power assay and ferrous chelating method.

Conclusion: The extract showed a dose-dependent significant antibacterial and antioxidant activity.

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References

Sastri BN. The Wealth of India. (Publication and Information Directorate, CSIR. Hillside. New Delhi, India; 1962. p. 336.

Dhar ML, Dhar MC, Dhawan BN, Mehtrotra BN, Ray C. Screening of Indian plants for biological activity. Indian J Exp Biol 1968;2:232-47.

Thapliyal RP, Bahuguna RP. An oleanolic acid based bis glycoside from Clematis montana roots. Phytochemistry 1993;34:861-2.

Bahuguna RP, Thapliyal RP, Murakami N, Tanase T, Kaiya T, Sakakibara. Saponins from Clematis Montana. Pharm Biol 1990;28:125-7.

Thapliyal RP, Bahuguna RP. Clemontanoside-C. A saponin from Clematis montana. Phytochemistry 1993;33:671-3.

Shivaprasad HN, Mohan S, Kharya MD, Shiradkar RM, Lakshman K. In vitro models for antioxidant activity evaluation: a review. Latest Rev 2005;3:981-96.

C Soler-Rivas, JC Espin, HJ Wichers. An easy and fast test to compare total free radical scavenger capacity of foodstuffs. Phytochem Anal 2000;11:330-8.

B Halliwell. Free radicals, antioxidants, and human disease: curiosity, cause, or consequence? Lancet 1994;344:721-4.

JC Espin, C Soler-Rivas, HJ Wichers. Characterization of the total free radical scavenger capacity of vegetable oils and oil fractions using 2,2-diphenyl-1-picrylhydrazyl radical. J Agric Food Chem 2000;48:648-56.

RL Prior. Fruits and vegetables in the prevention of cellular oxidative damage. Am J Clinic Nutr 2003;78(3 Suppl)570S-578S.

Aiyegoro OA, Okoh AI. Preliminary phytochemical screening and In vitro antioxidant activities of the aqueous extract of Helichrysum longifolium DC. BMC Complementary Altern Med 2010;10:1â€8.

Kokate CK. Practical pharmacognosy. Vallabh prakashan, New Delhi, India; 2005. p. 107-11.

Indian Herbal Pharmacopoeia. Vol. I. Tanushree Enterprises, Worli Naka, Mumbai; 2007. p. 179-81.

Indian Pharmacopoeia. Vol. II. The Controller of Publications, New Delhi; 1996. p. A52-A54.

WHO/PHARM/92.559/rev. 1. Quality Control Methods for Medicinal Plant Materials, Organisation Mondiale De La Sante, Geneva: 1992. p. 22-34.

Curcic MG, Stankovic MS, Radojevic ID, Stefanovic OD, Comic LR, Topuzovic MD, et al. Biological effects, total phenolic content and Flavanoid concentrations of fragrant yellow onion (Allium flavum L.). Med Chem 2012;89:1-6.

Robu S, Aprotosoaic AC, Miron A, Cioanca O, Stanescu U, Hancianu M. In vitro antioxidant activity of ethanolic extracts from some Lavandula species cultivated in Domania. Farmacia 2012;60:394-401.

Maisarah AM, Nurul Amira B, Asmah R, Fauziah O. Antioxidant analysis of different parts of Carica papaya. Int Food Res J 2013;20:1043-8.

Reeves, Wise, Andrews and White. Clinical Antimicrobial Assay. Oxford University, New York; 1999. p. 24-5.

Moein MR, Moein S, Ahmadizadeh S. Radical scavenging and reducing the power of Salvia mirzayanii subfractions. Molecules 2008;13:2804–13.

Fukomoto LR, Mazza G. Assessing antioxidant and prooxidant activities of phenolic compounds. J Agric Food Chem 2000;48:3597-604.

Bors W, Michel C. Antioxidant capacity of flavanols and gallate esters: pulse radiolysis studies. Free Radical Biol Med 1999;27:1413-26.

Published

18-10-2016

How to Cite

Bhatt, H., S. Saklani, and K. Upadhayay. “PRELIMINARY PHYTOCHEMICAL, PHYSICOCHEMICAL, ANTIOXIDANT AND ANTIMICROBIAL STUDIES OF CLEMATIS MONTANA LEAVES”. International Journal of Current Pharmaceutical Research, vol. 8, no. 4, Oct. 2016, pp. 33-36, doi:10.22159/ijcpr.2016v8i4.15274.

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