MICROBIAL EXTRACTION OF CHITIN AND CHITOSAN FROM PLEUROTUS SPP, ITS CHARACTERIZATION AND ANTIMICROBIAL ACTIVITY
DOI:
https://doi.org/10.22159/ijcpr.2017v9i1.16623Keywords:
Chitin, Chitosan, FTIR, SEM, Pleurotus spp, Antibacterial activityAbstract
Objective: The aim of the present work was to extract the chitin and chitosan from two species of Pleurotus, its characterization and its antimicrobial activity against bacteria and fungi.
Methods: The fungi were grown in MGYP medium for 15 d. The chitin and chitosan were extracted using the alkaline method and various biochemical methods and compared with commercial chitin and chitosan. The FTIR and SEM were also performed. Antimicrobial activity was performed using well diffusion method.
Results: The maximum yield of mycelia and the chitin and chitosan were obtained on 12th day of incubation. P. folrida gave maximum yield (201.3 mg/l of chitin and 65.61 mg/l of chitosan). Fourier Transform Infrared spectroscopy comparing the results obtained with results for commercial chitin and chitosan. Our results showed that chitin and chitosan obtained from Pleurotus spp. had a significant similitude with commercial. SEM image also carried out to study the surface morphology. The fungal chitin and chitosan had more antimicrobial activity against the gram+ve, gram–ve and the various fungus.
Conclusion: In the present study the chitin and chitosan were extracted from the Pleurotus spp. (P. florida and P. eous). The yield was maximum in P. florida after 12thday of fermentation. The FTIR and SEM also confirmed the chitin and chitosan. The extracted chitin and chitosan were subjected to antimicrobial activity, and it was found the extracted chitin and chitosan were active against the bacteria and fungi. Our findings suggest that the Pleurotus spp. are the potential candidate to produce eco-friendly chitin and chitosan in the development of drugs, artificial bone and raw material for the food industries in the near future.
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References
Lower SE. Polymers from the sea chitin and chitosan. Manuf Chem 1984;55:73-5.
Skaugrud O, Sargent G. Chitin and chitosan: crustacean biopolymers with potential application. International By-pro-ducts Conference, Anchorage, Alaska; 1990. p. 61-72.
Fereido on Shahidi, Janak Kamil Vidana Arachchi, You-Jin Jeon. Food applications of chitin and chitosan. Trends Food Sci Technol 1999;10:37-51.
Wan ACA, Tai BCU. CHITIN-A promising biomaterial for tissue engineering and stem cell technologies. Biotechnol Adv 2013;31:1776–85.
Dutta PK, Ravikumar MNV, Dutta J. Chitin and chitosan for versatile applications. Polym 2002;42:307–54.
Caprile MD. Obtencion y utilizacion de quitina y quitosano a partir de desechos de crusta´ceos. Congreso Mundial ISW: Haciaunsistema integral de residuossolidosurbanos, Argentina; 2005.
Natali Mesa Ospina, Sandra Patricia Ospina Alvarez, Diana Marcela Escobar Sierra, Diego Fernando Rojas Vahos, Paola Andrea Zapata Ocampo, Claudia Patricia Ossa Orozco. Isolation of chitosan from Ganodermalucidum mushroom for biomedical applications. J Mater Sci: Mater Med 2015;26:135.
HK No, Meyers SP, W Prinyawiwatkul, Z Xu. Applications of chitosan for improvement of quality and shelf life of foods: a review. J Food Sci 2007;72:R87–100.
Teng WL, Khor E, Tan TK, Lim LY, Tan SL. Concurrent production of chitin from shrimp shells and fungi. Carbohydr Res 2001;332:305-16.
Chatterjee S, Adhya M, M Guha, AK Chatterjee. BP chitosan from Mucorrauxii spp. production and physio–chemical charectorisation. Process biochem; 2004.
Percot A, Viton C, Domard A. Optimization of chitin extraction from shrimp shells. Biomacromolecules 2003;4:12-8.
Dutta PK, Tripath S, Mehrotra GK, Dutta J. Perspective of chitosan-based antimicrobial films in food applications. Food Chem 2009;114:1173–82.
Kannan M, Nesakumari M, Rajarathinam K, Singh AJAR. Production and characterization of mushroom chitosan under solid–state fermentation conditions. Adv Biol Res 2010;4:10-3.
Vaingankar PN, Juvekar AR. Fermentative production of mycelial chitosan from zygomycetes: media optimisation and physicochemical characterization. Adv Biosci Biotechnol 2014;5:940-56.
Yang N, Li W. Facile one-pot synthesis of chitosan oligosaccharide/silver nanocomposites and their antimicrobial properties. Mater Lett 2014;132:145–8.
Krishnaveni B, Ragunathan R. Extraction and characterization of Chitin and Chitosan from Aspergillusterreusspp. synthesis of their bionanocomposites andstudy of their productive applications. J Chem Pharm Res 2015;7:115-32.
Sandra Patricia Ospina-Alvarez, David Alexander Ramirez Cadavid, Diana Marcela Escobar Sierra, Claudia Patricia Ossa Orozco, Diego Fernando Rojas Vahos, Paola Zapata Ocampo, et al. Comparison of extraction method of chitin from Ganodermalucidum mushroom obtained in submerged culture. Hindawi publishing corporation BioMed Res Int 2014:7. http://dx.doi.org/10.1155/2014/169071
Hu KJ, Hu JL, Ho KP, Yeunj KW. Screening of fungi for chitosan producers, and copper adsorption capacity of fungal chitosan and chitosanaceous materials. Carbohydratepolymers 2004;58:45-52.
Pochanavanich P, Suntornsuk W. Fungal chitosan production and its characterization. Department of Microbiology, King Mongkut’s the University of Technology. Thon buri, Thailand. Appl Microbiol 2002;35:17–21.
Ozden Canli Tasara, Serkan Erdalb, Mesut Taskin. Chitosan production by psychrotolerant Rhizopusoryzaein non-sterile open fermentation conditions. Int J Biol Macromol 2016;89:428–33.
Maghsoodi V, Yaghmaie S. Influence of different nitrogen sources on the amount of chitosan production by Aspergillusniger in solid state fermentation. Iran J Chem Chem Eng 2008;27:47-52.
Mohammad Ali Ebrahimzadeh, Aroona Cabra, Eshrat Gharaei-Fathabad, Fereshteh Pourmorad. Preparation of chitosan from Penicillium spp. And the determination of their degree of deacetylation. Indian J Biotechnol 2013;12:231-5.
Nadarajah K, Kader J, Mazmira M, Paul DC. Production of chitosan from fungi. Pak J Biol Sci 2001;4:263-5.
Paulino T, Simionato JI, Garcia JC, Nozaki J. Characterization of chitosan and chitin produced from silkworm chrysalides. Carbohydr Polym 2006;64:98–103.
Kasaai MR, Arul J, Charlet G. Intrinsic viscosity-molecular weight relationship for chitosan. J Polym Sci Part B: Polym Phys 2000;38:2591-8.
Adam Waskoa A, Piotr Bulakb, Magdalena Polak-Bereckaa, Katarzyna Nowakb, Cezary Polakowskib, Andrzej Bieganowski. The first report of the physicochemical structure of chitin isolated from Hermetiaillucens. Int J Biol Macromol 2016;92:316–20.
Suneeta Kumari, Pradip Kumar Rath. Extraction and characterization of chitin and chitosan from (Labeorohit) fish scales. Procedia Mater Sci 2014;6:482–9.
Erdogan S, Kaya M, Mol T, Baran T. Comparison of chitin structures isolated from seven Orthoptera species. Int J Biol Macromol 2015;72:797–805.
Helander I, Nurmiaho-Lassila E, Ahvenainen R, Rhoades J, Roller. Chitosan disrupts the barrier properties of the outer membrane of Gram-negative bacteria. Int J Food Microbiol 2001;71:235-44.
KAbu Tareq, Masihul Alam, Salim Raza, Tanvir Sarwar, Z Fardous, Alamgir Z Chowdhury, et al. Comparative study of antibacterial activity of chitin and chemically treated chitosan prepared from shrimp (Macrobrachium Rosenbergii) shell waste. J Virol Microbiol 2013:9. Doi:10.5171/2013.369217
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