ISOLATION AND IDENTIFICATION OF STEROIDS FROM DIFFERENT PARTS OF PROSOPIS JULIFLORA
DOI:
https://doi.org/10.22159/ijcpr.2017.v9i3.18896Keywords:
Prosopis juliflora, GC-MS, Steroids, Diosgenin, Thin Layer ChromatographyAbstract
Objective: The present study was carried out to investigate the steroid content present in the leaves, stem, pods and callus of Prosopis juliflora.
Methods: The method of Tomita et al., was used for isolation of steroids. The structure of the isolated compound was established on the basis of physical and chemical test and spectroscopic evidence (TLC, IR and GC-MS).
Results: The study concluded that a single type of steroid Diosgenin was found in the selected plant species.
Conclusion: Diosgenin is an important steroidal metabolite used as a starting material for the synthesis of steroidal drugs, as it exhibits estrogenic activity.
Downloads
References
Khandelwal Preeti, Sharma Ram Avatar, Agarwal Mala. Pharmacology and therapeutic application of Prosopis juliflora: a review. J Plant Sci 2015;3:234-40.
Khandelwal Preeti, Sharma Ram Avatar, Agarwal Mala. Pharmacology, phytochemistry and therapeutic application of Prosopis cineraria linn: a review. J Plant Sci 2015;3:33-9.
Oduor NM, Githiomi JK. Fuel-wood energy properties of Prosopis juliflora and Prosopis pallida grown in Baringo District, Kenya. Afr J Agric Res 2013;8:2476-81.
Hardman R. Recent advances in plants of pharmacological importance. Steroidal Aspects. Egypt J Pharm Sci 1980;21:169.
Coppen JJW. Steroids from plants to pills-The changing the picture. Trop Sci 1979;21:125-41.
Blunden G, Carabot A, Jewers K. Steroidal sapogenins from leaves of some species of Agave and Furcraea. Phytochemistry 1980;19:2489-90.
Tomita Y, Uomori A, Minato H. Steroidal sapogenins and sterols in tissue cultures of Dioscorea tokoro. Phytochem 1970;9:111.
Bennett RD, Haftmann RD. Thin layer chromatography of steroidal sapogenins. J Chromato 1962;9:353.
Heble MR, Narayanaswami S, Chadha MS. Diosgenin and β-sitosterol; Isolation from Solanum xanthocarpum tissue cultures. Science 1968;161:1145.
Khanna P, Jain SC. Diosgenin, gitogenin and tigogenin from Trigonella foenum-graecumL tissue cultures. Lloydia 1973;36:96-8.
Kaul B, Staba EJ. Dioscorea tissue cultures Biosynthesis and isolation of diosgenin from Dioscorea deltoidea callus and suspension cells. Lloydia 1968;31:171-9.
Velmurugan V, Arunachalam G, Ravichandran V. Antibacterial activity of stem bark of Prosopis cineraria (Linn.) druce. Arch Appl Sci Res 2010;2:147-50.
Divi UK, Krishna P. Brassinosteroid: a biotechnological target for enhancing crop yield and stress tolerance. New Biotechnol 2009;26:131-6.
Aradhana M, Rao AC, Kale RK. Diosgenin a growth stimulator of the mammary gland of ovariectomized mouse. Indian J Exp Biol 1992;30:367-70.
Roman TD, Thewles A, Coleman R. Fractionation of liver following diosgenin treatment to elevate binary cholesterol. Biochem Biophys Acta 1995;1255:77-81.
Sarvaire Y, Ribes G, Baccou JC. The implication of steroid saponins and sapogenins in the hypocholesterolemic effect of fenugreek. Lipids 1991:26.191-7.
Published
How to Cite
Issue
Section
