PRELIMINARY PHYTOCHEMICAL SCREENING AND IN VITRO ANTIMICROBIAL ACTIVITY OF ETHANOLIC EXTRACT OF WHOLE AERIAL PART OF THE HERB LEUCAS PLUKENETII SPRENG (FAMILY-LAMINACEAE)

Uttam Borah; Biswajit Dash; Suvakanta Dash; Lawrence Kalita

doi:10.22159/ijcpr.2017.v9i3.19598

Authors

Uttam Borah Girijananda Chowdhury Institute of Pharmaceutical Science, Azara, Guwahati 781017
Biswajit Dash
Suvakanta Dash
Lawrence Kalita

DOI:

https://doi.org/10.22159/ijcpr.2017.v9i3.19598

Keywords:

Leucas Plukenetii, Antimicrobial, Zone of Inhibition, Agar disc diffusion method

Abstract

Objective: To evaluate the antimicrobial activity of ethanolic extract of Leucas Plukenetii Spreng (family-laminaceae) in conjugation with phytochemical analysis.

Methods: The ethanolic extract of whole aerial parts of the herb Leucas Plukenetiispreng (family-laminaceae) was prepared and analysed for phytochemical constituents using standard methods. The antimicrobial activity of the plant extract was examined against 2 bacterial strains among one is gram positive and other is gram negative and 2 fungal strains using agar well diffusion method.

Results: The present investigation shows the phytochemical analysis, antimicrobial activity of the ethanolic extract of the plant Leucas Plukenetii Spreng. Various phytochemical analyses revealed the presence of alkaloids, saponin, flavonoids, carbohydrates, glycosides, Steroids, proteins and amino acids and tannins. The antimicrobial activity of the ethanolic extract of the plant showed significant result against all the of the test organisms.

Conclusion: The present study concluded that ethanolic extract of whole aerial parts of the Leucas Plukenetii Spreng contains the high presence of phytochemicals. The ethnaolic extract of the plant was found to possess promising antimicrobial activity when compared with the standards.

Downloads

Download data is not yet available.

References

Sukanya SL, Sudisha J, Hariprasad P, Niranjana SR, Prakash HS, Fathima SK. Antimicrobial activity of leaf extracts of Indian medicinal plants against clinical and phytopathogenic bacteria. Afr J Biol 2009;8:6677-82.

Mothana RA, Lindequist U. Antimicrobial activity of some medicinal plants of the island Soqotra. J Ethnopharmacol 2005;96:177-81.

Chanda S, Rakholiya K. Combination therapy: synergism between natural plant extracts and antibiotics against infectious diseases. Microbiol Book Series; 2011. p. 520-9.

Harishchandra MR, Rajan PR, Singh Satyendrakumar RP. Study of krimighna effect of nimba (Azadiracta indica A. Juss.) patra as rakshoghna dhoopan by culture and sensitivity method W. S. R. to pyogenic bacteria. Int Res J Pharm 2012;3:142-6.

Nascimento GF, Lacatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian J Microbiol 2000;31:886-91.

Dahanukar SA, Kulkarni RA, Rege NN. Pharmacology of medicinal plants and natural products. Indian J Pharmacol 2000;32:81-118.

Silva MSP, Brandao DO, Chaves TP, Filho ALNF, Costa EMDB, Santos VL, et al. Study bioprospecting of medicinal plant extracts of the semiarid northeast: contribution to the control of oral microorganisms. J Evidence-Based Complementary Altern Med 2012;2012:1-6.

Ncube B, Finnie JF, Staden JV. In vitro antimicrobial synergism within plant extract combinations from three South African medicinal bulbs. J Ethnopharmacol 2012;139:81-9.

Hemalatha M, Thirumalai T, Saranya R, Elumalai EK, David E. A review on antimicrobial efficacy of some traditional medicinal plants in Tamilnadu. J Acute Disease 2013;2:99-105.

Gowrish A, Vagdevi HM, Rajashekar H. Phytochemical screening and antimicrobial activity of Leucas marrubioides desf. root extracts. Int J Pharm Pharm Sci 2016;8:209-10.

Chouhan HS, Singh SK. A review of plants of genus Leucas. J Pharmacogn Phytother 2011;3:13-26.

Tiwari P, Kumar B, Kaur M, Kaur G, Kaur H. Phytochemical screening and extraction: a review. Int Pharm Sci 2011;1;98-106.

National Committee for Clinical Laboratory Standards. Performance Standards for Antimicrobial Disk Susceptibility Tests. Fifth Edition: Approved Standard M2-A5. NCCLS, Villanova, PA; 1993a.

Kurian S, Josekumar VS. Phytochemical screening, anti-microbial activity and brine shrimp lethality bioassay of different extracts of Alysicarpus vaginalis var. nummularifolius (DC.) MIQ. (family: fabaceae). Int J Pharm Sci 2017;9:1-6.