ANTIBACTERIAL ACTIVITY OF SYNTHESIZED SILVER NANOPARTICLES BY SIMAROUBAGLAUCA AGAINST PATHOGENIC BACTERIA
DOI:
https://doi.org/10.22159/ijcpr.2017v9i4.20629Keywords:
Simaroubaglauca, Silver nanoparticles, Leaf extract, Bioreductant, AntibacterialAbstract
Objective: The present study outline the plant-mediated synthesis of silver nanoparticles (AgNPs) using leaf extract Simaroubaglauca, which act as both reducing and stabilizing agent.
Methods: Formation of silver nanoparticles was confirmed by primarily by Ultraviolet/visible spectroscopy. X-ray diffraction studies revealed the crystallinity of the nanoparticles. The scanning electron microscopy was carried out to determine the mean particle size, as well as the morphology of the NPs and the composition of elements, was studied with Energy Dispersive X-ray analysis (EDS).
Results: The silver nanoparticles were spherical in shape with a mean size of 23 nm. The EDS showed strong optical absorption peak at 3keV and it was confirmed the formation of AgNPs. The synthesised AgNPs further utilized for the evaluation of antibacterial activity and shown significant antibacterial activity against Escherichia coli, Pseudomonas aeruginosa, Enterobacter and Klebsiella pneumonia at 50 µg/ml and 100µg/ml concentrations.
Conclusion: The synthesised silver nanoparticles have been characterised by UV-vis, SEM-EDAX and XRD to determine the sizes and shapes of the silver nanoparticles.
Downloads
References
Benjamin LO, Francesco S. Antibacterial activity of silver nanoparticles: a surface science insight. Nano Today 2015;10:339-54.
Swarnali M, Deepak K, Gadadhar B, Sudip KG, Jayasree KL. Antimicrobial activities of silver nanoparticles synthesized from Lycopersiconesculentum extract. J Anal Sci Technol 2014;5:40:1-7.
Parikh DV, Fink T, Rajasekharan K, Sachinvala ND, Sawhney APS, Calamari TA, et al. Antimicrobial silver/sodium carboxymethyl cotton dressings for burn wounds. Text Res J 2005;75:134–8.
Ulkur E, Oncul O, Karagoz H, Yeniz E, Celikoz B. Comparison of silver-coated dressing (ActicoatTM), chlorhexidine acetate 0.5% (Bactigrass®), and fusidic acid 2% (Fucidin®) for topical antibacterial effect in methicillin-resistant staphylococci-contaminated, full-skin-thickness rat burn wounds. Burns 2005;31:874–7.
Jeong SH, Yeo SY, Yi SC. The effect of filler particle size on the antibacterial properties of compounded polymer/silver fibres. J Mater Sci 2005;40:5407–11.
Lee HY, Park HK, Lee YM, Kim K, Park SB. A practical procedure for producing silver nanocoated fabric and its antibacterial evaluation for biomedical applications. Chem Commun 2007;28:2959–61.
Yuranova T, Rincon AG, Bozzi A, Parra S, Pulgarin C, Albers P, et al. Antibacterial textiles prepared by RF-plasma and vacuum-UV mediated deposition of silver. Photochem Photobiol 2003;161:27–34.
Samuel U, Guggenbichler JP. Prevention of catheter-related infections: the potential of a new nanosilver-impregnated catheter. Int J Antimicrob Agents 2004;23:75–8.
Alt V, Bechert T, Streinrucke P, Wagener M, Seidel P, Dingeldein E, et al. Domanne E, schnettler r an in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials 2004;25:4383–91.
Rupp ME, Fitzgerald T, Marion N, Helget V, Puumala S, Anderson JR, et al. Effect of silver-coated urinary catheters: efficacy, cost-effectiveness antimicrobial resistance. Am J Infect Control 2004;32:445–50.
Chenousova S, Epple M. Silver as an antibacterial agent: ion, nanoparticle, and metal. Angew ChemInt Ed Engl 2013; 4:1636-53.
Kalsen HJ. Historical review of the use of silver in the treatment of burns. I. Early uses. Burns 2000;26:2:117-30.
Fuhrmann GF, Rothstein A. The mechanism of the partial inhibition of fermentation in yeast by nickel ions. Biochim Biophys Acta 1968;163:331-8.
Miller LP, Mc Callan SEA. The toxic action of metal ions to fungus spores. J Agric Food Chem 1957;5:116-22.
Rayman MK, LoT C, Sanwal BD. Transport of succinate in Escherichia coli. II. Characteristics of uptake and energy coupling with transport in membrane preparations. J Biol Chem 1972;247:6332-9.
Schreurs WJ, Rosenberg H. Effect of silver ions on transport and retention of phosphate by Escherichia coli. J Bacteriol 1982;152:7-13.
Joseph JD, Jeffrey TB, Jude CO, Peter MD, Oliver NM, Ibrahim M. Antimicrobial efficacy of biosynthesized silver nanoparticles from different solvent extracts of Waltheriaamericana root. J Anal Sci Technol 2016;7:23.
Santhana LK, Sangeetha D, Sivamani S, Tamilarasan M, Rajesh TP, Anandraj B. In vitro antibacterial, antioxidant, haemolytic, thrombolytic activities and phytochemical analysis of Simaroubaglauca leaves extracts. Int J Pharm Sci Res 2014;5:432-7.
Nagaraj B, AgnieszkaSobczak-Kupiec, Rebecca IF, Salman D. Bioreduction of chloroaurate ions using fruit extract Punicagranatum (Pomegranate) for synthesis of highly stable gold nanoparticles and assessment of its antibacterial activity. Micro Nano Lett 2013;8:400–4.
Nithya R, Ragunathan R. Synthesis of the silver nanoparticle using pleurotus Sajorcaju and its antimicrobial study. Digest J Nanomaterials Biostructures 2009;4:623-9.
Chandrappa CP, Govindappa M, Chandrasekar N, Sonia S, Sepuri O, Channabasava R. Endophytic synthesis of silver chloride nanoparticles from Penicillium sp. of Calophyllumapetalum. Adv Nat Sci: Nanosci Nanotechnol 2016;7:1-5.
Nagaraj B, Akber I, Yong RL. Preparation of Au and Ag nanoparticles using Artemisia annua and their in vitro antibacterial and tyrosinase inhibitory activities. Mater Sci Eng Carbon 2014;43:58–64.
Baker RA, Tatum JH. Novel anthraquinones from stationary cultures of Fusariumoxysporum. J Ferment Bioeng 1998; 85:359-61.
Mritunjai S, Shinjini S, Prasad S, Gambhir IS. Nanotechnology in medicine and antibacterial effect of silver nanoparticles. Digest J Nanomater Biostructures 2007;3:115-22.
Published
How to Cite
Issue
Section
