ANTIOXIDANT ACTIVITIES OF SOME LESS UTILIZED EDIBLE FRUITS
DOI:
https://doi.org/10.22159/ijcpr.2017v9i5.22132Keywords:
Less utilized fruits, Total phenolic, Total flavonoid, Spondias pinnataAbstract
Objective: The objective of the present study was to evaluate the total phenolic (TPC) and flavonoid content (TFC) of five less utilized fruits such as Aegle marmelos, Spondias pinnata, Limonia acidissima, Averhoa carambola, Crescentia cujete and was compared with Phyllanthus emblica, (Amla) well known for its antioxidant activities. Total phenolic and flavonoid contents of samples were correlated with antioxidant activities like 1,1-Diphenyl-2-Picryl-Hydrazyl (DPPH) free radical scavenging assay, Ferric reducing antioxidant potential (FRAP) assay and total antioxidant capacity (TAC).
Methods: The total phenolic of each fruit extract were determined by the Folin-Ciocalteu method with some modifications and the total flavonoids were estimated by Aluminum trichloride colourimetric method. The DPPH antioxidant assay, The FRAP assay and TAC were determined spectrophotometrically.
Results: The total phenolics were expressed as mg/100g Gallic acid equivalent (mg GAE/100 gm) and the total flavonoids were expressed as mg/100g Quercetin equivalent (mg QE/100 gm). TPC was found to be maximum in Spondias pinnata with 142.16 mg GAE/100 gm where as TFC was maximum in Phyllanthus emblica with 91.1 mgQE/100 gm. DPPH radical scavenging activity was expressed n percentage(%), FRAP values expressed as mg/100g Ascorbic equivalent (AAE) and the total antioxidant activity was expressed as mg/100g Ascorbic equivalent. Maximum DPPH radical scavenging activity was shown by Spondias pinnata (93.75%), FRAP values were maximum in Phyllanthus emblica with 72.6 mg AAE/100 gm and total antioxidant capacity was found to be highest in Spondias pinnata (50.1 mg AAE/100 gm).
Conclusion: Spondias pinnata, an underutilized fruit, was found to be promising with antioxidant activities comparable to Phyllanthus emblica.
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References
Scalzo J, Politi A, Pellegrini N, Mezzetti B, Battino M. Plant genotype affects total antioxidant capacity and phenolics contents in fruit. Nutrition 2005;21:207-13.
Hassimotto NMA, Genovese MI, Lajolo FM. Antioxidant activity of dietary fruits, vegetables and commercially frozen fruit pulps. J Agric Food Chem 2005;53:2928-35.
Abdel-Hameed ESS. Total phenolics contents and free radical scavenging activity of certain eyptian ficus species leaf samples. Food Chem 2009;114:1271-7.
Giasson BI, Ischiropoulos H, Virginia M, Lee Y, Trojanowski JQ. The relationship between oxidative/nutritive stress and pathological inclusions in Alzheimer's and Parkinson's diseases. Free Radical Biol Med 2002;32:1264-75.
Pietta PG. Flavonoidss as antioxidants. J Nat Prod 2000;63:1035-42.
Ozga JA, Saeed A, Wismer W, Reinecke DM. Characterization of cyanidin-and quercetin-derived Flavonoids and other Phenolicsss in mature saskatoon fruits (Amelanchier alnifolia Nutt.). J Agric Food Chem 2007;55:10414-24.
Karuppusamy S, Muthuraja G, Rajasekaran KM. Antioxidant activities of selected lesser known edible fruits of from Western Ghats of India. Indian J Nat Prod Resources 2011;2:174-8.
Hegazy AK, Al–Rowaily SL, Faisal M, Alatar AA, El-Bana MI, Assaeed AM. Nutritive value and antioxidant activity of some edible wild fruits in the Middle East. J Med Plants Res 2013;7:938-46.
Nazarudeen A. Nutrional composition of some lesser-known fruits used by the ethnic communities and local folks of Kerala. Indian J Traditional Knowledge 2010;9:398-402.
Bhattacharya A, Chatterjee A, Ghosal S, Bhattacharya SK. Antioxidant activity of active tannoid principles of Emblica officinalis (amla). Indian J Exp Biol 1999;37:676-80.
Khopde SM, Indira Priyadarsini K, Mohan H, Gawandi VB, Satav JG, Yakhmi JV, et al. Characterizing the antioxidant activity of amla (Phyllanthus emblica) extract, Curr Sci 2001;81:185-90.
Arvouet-Grand A, Vennat B, Pourrat A, Legret P. Standardisation d'un extrait de propolis et identification des principaux. J Pharm Belgique 1994;49:462-8.
Shimada K, Fujikawa K, Yahara K, Nakamura T. Antioxidative properties of xanthan on the autoxidation of soybean oil in cyclodextrin emulsion. J Agric Food Chem 1992;40:945-8.
Oyaizu M. Studies on the product of browning reaction prepared from glucose amine. Jpn J Nutr 1986;44:307-15.
Prieto P, Pineda M, Anguilar M. Spectrophotometric quantitation of antioxidant capacity through the formation of a phosphomolybdenum complex: specific application to the determination of Vitamin E. Anal Biochem 1999;269:337-41.
Rice-Evans CA, Miller NJ, Bolwell PG, Bramley PM, Pridham JB. The relative antioxidant activities of plant-derived poly phenolics flavonoids. Free Radical Res 1995;22:375-83.
Olajire AA, Azeez L. Total antioxidant activity, Phenolics, Flavonoids and ascorbic acid contents of Nigerian vegetables. Afr J Food Sci Technol 2011;2:22-9.
Chalise JP, Acharya K, Gurung N, Bhusal RP, Gurung R, Skalko-Basnet N, et al. Antioxidant activity and polyphenol content in edible wild fruits from Nepal. Int J Food Sci Nutr 2010;4:425-32.
Pietta PG. Flavonoidss as antioxidants. J Nat Prod 2000;63:1035-42.
Soares JR, Dinis TC, Cunha AP, Almeida LM. Antioxidant activity of some extracts of Thymus zygi. Free Radical Res 1997;26:469-78.
Ferreira ICFR, Bptista P, Vilas-Boas M, Barros L. Free-radical scavenging capacity and reducing the power of wild edible mushrooms from northeast portugal: individual cap and stipe activity. Food Chem 2007;100:1511-6.
Shanmugam S, Kumar Sathish T, Panneer Selvam K, Modified ferric ion reducing antioxidant power assay (Modified FRAP Assay). In: laboratory handbook on Biochemistry PHI Learning Pvt. Ltd; 2010. p. 116-7.
Gzella AG, Makuch MD, Matławska I. DPPH radical scavenging activity and Phenolics compound content in different leaf extracts from selected blackberry species. Acta Biol Cracov Ser Bot 2012;54:32-8.
Kondo S, Kittikorn M, Kanlayanarat S. Pre harvest antioxidant activities of tropical fruit and the effect of low-temperature storage on antioxidants and jasmonates. Postharvest Biol Technol 2005;36:309-18.
Khomdram S, Arambam S, Shantibala Devi G. Nutritional profiling of two underutilized wild edible fruits Elaeagnus pyriformis and Spondias pinnata. Ann Agric Res New Series 2014;35:129-35.
Deshmukh BS, Waghmode A. Role of wild edible fruits as a food resource: Traditional knowledge. Int J Pharm Life Sci 2011;2:919-24.
