ANTIMICROBIAL ACTIVITY AND PHYTOCHEMICAL SCREENING OF THE LEAF EXTRACTS OF EUCALYPTUS GLOBULUS
DOI:
https://doi.org/10.22159/ijcpr.2017v9i5.22145Keywords:
Eucalyptus globulus, Phytochemical analysis, Antimicrobial activity, Microbroth dilution method, NilAbstract
Objective: To investigate the antimicrobial activity and phytochemical analysis of Eucalyptus globulus leaves.
Methods: The antimicrobial activity of the leaves of E. globulus extracted with acetone, ethanol and petroleum ether was studied using well diffusion method against the selected bacterial and fungal isolates. The antimicrobial susceptibility of the bacteria and fungi to various concentrations of the extract was evaluated by the micro broth dilution method. The preliminary phytochemical screening was performed in the acetone extract of E. globulus. The presence of functional groups in the acetone extract of the leaves of E. globulus was determined by Fourier to transform infrared (FT-IR) spectroscopy.
Results: Results suggested that the acetone extract exhibited maximum antimicrobial activity against the tested bacteria and fungi when compared with ciprofloxacin and fluconazole respectively (standard antibiotics). Minimum inhibitory concentration (MIC) showed that the wells containing a concentration of 12.5 mg/ml and 6.25 mg/ml of acetone extract inhibited the bacteria and fungi effectively. Phytochemical screening of acetone extract of E. globulus showed the presence of alkaloids, flavonoids, phenols, tannins, quinones, glycosides, steroids, terpenoids and leucoanthocyanides. FT-IR spectral analysis of the leaf extract of E. globulus showed the presence of functional groups such as–OH,-NH, C=O and–COOH.
Conclusion: Thus to conclude, different plants and their formulations have been used in ayurveda to cure diseases for several decades. The findings of the study indicate the potential to exploit the leaves of Eucalyptus globulus in the treatment of microbial diseases.
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Kiruba S, Mahesh M, Nisha SR, Miller Paul Z, Jeeva S. Phytochemical analysis of the flower extracts of Rhododendron arboretum Sm. Ssp. Nilagiricum (Zenker) Tagg. Asian Pac J Trop Biomed 2011;S:284-6.
Rao TG, Babu SK, Kumar UJ, Sujana P, Rao V, Sreedhar AS. Anti-microbial principles of selected remedial plants from Southern India. Asian Pac J Trop Biomed 2011;1:298-305.
Raja ARD, Jeeva S, Prakash JW, Johnson M, Irudayaraj V. Antibacterial activity of selected ethanomedicinial plants from South India. Asian Pac J Trop Med 2011;4:375-8.
Nostro A, Gemano MP, Dangelo V, Marino A, Cannatelli MA. Extraction methods and bioautography for evaluation of medicinal plants antimicrobial activity. Lett Appl microbiol 2000;30:379-84.
Ayyanar I. Herbal medicines of wound healing among tribal people in Southern India: Ethnobotanical and Scientific Evidences. Int J Appl Res Nat Prod 2009;2:29-42.
Chakraborthy A, Brantner AH. Antibacterial activity of the stem bark of Holarrhenapubescens (Syn. H. antidysenterica). J Ethnopharmacol 1999;68:339.
Bandow JE, Brotz H, Leichert LO. Proteomic approach to understanding antibiotic action. Antimicrob Agents Chemother 2003;47:948-55.
Santos PRV, Oliveira ACX, Tomassini TCB. Control microbiogicodeproductos. Fitoterapicos. Rev Farm Bioquim 1995;31:35-8.
Westh H, Zinn CS, Rosdahl VT. Sarisa study group: an international multicenter study of antimicrobial consumption and resistance in Staphylococcus aureus. Microb Drug Resist 2004;10:169-76.
Afloyan AJ. Extracts from the shoots of Arctotis arctotoides inhibit the growth of bacteria and fungi. Pharm Biol 2003;41:22-5.
Musyimi DM, Ogur JA. Comparative assessment of antifungal activity of extracts from Eucalyptusglobulusand Eucalyptuscitriodora. Res J Phytochem 2008;2:35-43.
Barchiesi F, Colombo AL, McGough DA, Fothergill AW, Rinaldi MG. In vitro activity of itraconazole against fluconazole-susceptible and resistant Candida albicans isolates from oral cavities of patients infected with human immunodeficiency virus. Antimicrob Agents Chemother 1994,38:1530-3.
Harborne JB. Phytochemical methods: a guide to modern technique of plant analysis. London: Chapman and Hall; 1973. p. 271.
Naumann D. Infrared spectroscopy in microbiology. Meyers RA. ed. Encyclopedia of Analytical Chemistry. John Wiley and Sons, Chichester UK; 2000. p. 102-31.
Huffman BA, Poltash ML, Hughey CA. Effect of polar protic and polar aprotic solvents on negative-ion electrospray ionization and chromatographic separation of small acidic molecules. Anal Chem 2012;84:9942–50.
Bello M, Olabanji I, Ibrahim A, Yekeen T, Oboh L. Nutraceuticals in leaves of Eucalyptus citriodora and Eucalyptus camandulensis. Int J Chem Environ Biol Sci 2013;3:2320-87.
Tepe B, Donmez E, Unlu M, Candan F, Daferera D, Vardar-Unlu G. Antimicrobial and antioxidative activities of the essential oils and methanol extracts of Salvia cryptantha (Montbret et Aucher ex Benth.) and Salvia multicaulis (Vahl). Food Chem 2004;84:519–25.
Lupo S, Tiscornia S, Bettucci L. Endophytic fungi from flowers, capsules and seeds of Eucalyptus globulus. Rev Iberoamericana Micologıa 2001;181:38–41.
Bonjar SGH. Evaluation of antibacterial properties of Iranian medicinal plants against Micrococcus luteus, Serratiamarcescens, Klebsiellapneunomiae and Bordella bronchoseptica. Asian J Plant Sci 2004;3:82–6.
Thenmozhi DC, Ramalakshmi V. Preliminary phytochemical screening and Antibacterial activity of Pergularia daemia. Int J Pharma Bio Sci 2011;2:162-6.
Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
Alexei YB, Joseph IS, Olga VF. Endogenous cardiotonic steroids: physiology, pharmacology and novel therapeutic targets. Pharmacol Rev 2009;61:9–38.
Varghese A, Gavani U, Abraham S, Parambi BGT, Sathyanarayanan, Jose A. Phytochemical screening and antimicrobial investigation of Typhaangustifolia Linn. Int J Chem Sci 2009;7:1905-10.
Banso A, Adeyemo S. Phytochemical screening and antimalarial assessment of Abutilon mauritianum, Bacopa monnifera and Datura stramonium. Biokemistri 2006;18:39–44.
Vladimir K, Ludmila M. Glycosides in medicine: the role of glycosidic residue in biological activity. Curr Med Chem 2009;8:1303–28.
Aiyelaagbe OO, Osamudiamen PM. Phytochemical screening for active compounds in Mangifera indica leaves from Ibadan, Oyo State. Plant Sci Res 2009;2:11–3.
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