EVALUATION OF IN VITRO ANTI-INFLAMMATORY ACTIVITY AND HPTLC ANALYSIS OF PLANT PHYLLANTHUS FRATERNUS

##article.authors##

  • Biresh Kumar Sarkar Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
  • Ravi Kumar Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
  • Reeta . Central Ayurveda Research Institute for Respiratory Disorders, (CCRAS, Ministry of AYUSH, Govt. of India) Moti Bagh Road, Patiala, Punjab, India
  • S. C. Verma Principal Scientific Officer (Phyto/Org. Chemistry), PLIM Campus, Ghaziabad, U. P., India
  • Shashi Pal Manav Bharati University, Solan, HP, India
  • Ramaiah Maddi Department of Pharmacognosy, Hindu College of Pharmacy, Amaravathi Road, Guntur 522002, A. P., India
  • C. Murali Krishna Regional Ayurveda Research Institute for Skin disorders, (CCRAS, Ministry of AYUSH) Vijayawada, India
  • Ravindra Singh Central Council for Research in Ayurvedic Sciences (CCRAS), Ministry of AYUSH, Government of India, New Delhi 110058

##semicolon##

Anti-inflammatory##common.commaListSeparator## Phyllanthus Fraternus##common.commaListSeparator## HPTLC##common.commaListSeparator## Cyclooxygenase##common.commaListSeparator## Lipoxygenase

##article.abstract##

Objective: The present investigation evaluated in vitro anti-inflammatory activity of Phyllanthus fraternus. Inhibition of Cyclooxygenase and 5-lipoxygenase was performed along with protein denaturation.

Methods: Alcoholic extract of plant was subjected to in vitro anti-inflammatory activity and HPTLC analysis.

Results: The results of anti-inflammatory activity showed significant inhibition in Cyclooxygenase and 5-lipoxygenase assay, extract also showed more than 70 % inhibition in protein denaturation method. HPTLC of plant materials was also performed; spots of alkaloids were recorded.

Conclusion: Different alkaloids were spotted in chromatographic analysis and study suggested that anti-inflammatory activity of Phyllanthus fraternus may be due to the presence of alkaloids.

 

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##submissions.published##

21-09-2017

##issue.issue##

Vol 9, Issue 5 (Aug-Sep), 2017

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Original Article(s)