ANTI CANCER STUDIES OF SELECTIVE MANNICH BASES BY IN SILICO METHOD
DOI:
https://doi.org/10.22159/ijcpr.2018v10i2.25877Keywords:
Mannich bases, Docking, Estrogen, AnticancerAbstract
Objective: To evaluate the anticancer activities of selective Mannich bases by in silico methods.
Methods: X-ray crystallographic structure of Estrogen receptor protein (PDB ID 2YAT) was downloaded from the protein data bank (PDB) and is docked with the target Mannich bases using Accelyrs Discovery Studio client version 2.5 software.
Results: Based on the in silico analysis results of the target compounds with standard drug tamoxifen, the best-docked compound is identified and its anticancer activity is confirmed by using in vitro MTS analysis using Raju and Jurkat cell lines.
Conclusion: The mannich base compound N-[(Diphenylamino) methyl] acetamide showed fourfold higher activity than standard drug tamoxifen, may be used to overcome the drug resistance of Estrogen receptor protein.
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References
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