QUALITATIVE PHYTOCHEMICAL SCREENING AND FTIR SPECTROSCOPIC ANALYSIS OF GREWIA TILIFOLIA (VAHL) LEAF EXTRACTS
DOI:
https://doi.org/10.22159/ijcpr.2019v11i4.34936Keywords:
Grewia tiliaefolia (Vahl), Phytochemicals, Fourier-transform infrared spectroscopy (FTIR)Abstract
Objective: The present study aim is to analyse the phytochemicals present in Grewia tilifolia (Vahl) leaf extracts by using Qualitative phytochemical analysis, Fourier-transform infrared spectroscopy (FTIR).
Methods: The leaf extracts were prepared using eight different solvents. The phytochemical analysis Fourier transform infrared spectroscopy (FT-IR) analysis were performed using standard methods.
Results: The FTIR spectroscopic studies revealed different characteristic peak values with various functional groups present in the compounds of respective extracts. The FT-IR analysis shows the presence of different functional groups such as alcohols, phenols, alkanes, carboxylic acids, aldehydes, ketones, alkenes, primary amines, aromatics, esters, ethers, alkyl halides and aliphatic amine compounds, which showed major compounds present in the leaf extracts. The present study generated the FTIR spectrum profile for the medicinally important plant Grewia tilifolia.
Conclusion: The present study provides evediences that different extracts of Grewia tilifolia leaf is useful to cure many serious diseases which remained still problematic and for further isolation of bioactive compounds from the plant which could be of interest for the development of the new drug.
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29. Maobe MAG, Nyarango RM. Fourier transformer infra red spectrophotometer analysis of warburgia ugandensis medicinal herb used for the treatment of diabetes, malaria and pneumonia in kisii region, southwest kenya. Global J Pharmacol 2013;7:61-8.
2. R Ashok Kumar, M Ramaswamy. Phytochemical screening by FTIR spectroscopic analysis of leaf extracts of selected Indian medicinal plants. Int J Curr Microbiol Appl Sci 2014;3:395-406.
3. Visveshwari M, Subbaiyan B, Thangapandian V. Phytochemical analysis, antibacterial activity, FTIR and GCMS analysis of ceropegia juncea roxb. Int J Pharm Pharm Res 2017;9:914-20.
4. Hashimoto A, Kameoka T. Applications of infrared spectroscopy to biochemical, food, and agricultural processes. Appl Spectrosc Rev 2008;43:416–51.
5. Hussain K, Ismail Z, Sadikun A, Ibrahim P. Evaluation of metabolic changes in fruit of piper armentosum in various seasons by metabolomics using fourier transform infrared (FTIR) spectroscopy. Int J Pharm Clin Res 2009;1:68–71.
6. Srinivasan PA, Arokiaraj A, Vijayarajan D. Isolation, spectral and optical analysis of fistulic acid–a phytochemical constituent of cassia fistula linn. Indian J Sci Technol 2011;4:422–4.
7. Boydem MR. The NCI in vitro anticancer drug discovery screen. In: Teicher B. (Ed.). Anticancer drug development guide; preclinical screening, clinical trials and approval, Humana Press, Totowa; 1997. p. 30.
8. Brand WW, Cuvelier HE, Berset C. Use of the free radical method to evaluate antioxidant activity. Food Sci Technol 1995;82:25-30.
9. Cassady JM, Douros JD. Eds. Anticancer Agents Based on Natural Product Models. Academic Press, New York; 1980.
10. Anjaneyulu B, Baburao V, Ganguly AK, Govindachari TR, Joshi BS, Kamat VN, et al. Chemical investigation of some Indian plants. Indian J Chem 1965;3:237-8.
11. Badami S, Vijayan P, Mathew N, Chandrashekhar R, Godavarathi A, Dhanarajan SA, et al. In vitro cytotoxic properties of grewia tiliaefolia bark and lupeol. Indian J Pharmacol 2003;35:205-51.
12. Dhavan BN, Patnaik GK, Rastogi RP, Singh KK, Tandon JS. Screening of Indian plants for biological activity. Indian J Expt Biol 1977;15:208-19.
13. Chiruvella KK, Raghavan SC. A natural compound, methyl angolensate, induces mitochondrial pathway of apoptosis in Daudi cells. Invest New Drugs 2011;29:583-92.
14. N Sasidharan, BP Pal. Kerala Forest Research Institute, Peechi. Available from: https://indiabiodiversity.org/species/show/31410. [Last accessed on 10 Mar 2019]
15. Goyal PK. Phytochemical and pharmacological properties of the genus Grewia: a review. Int J Pharm Pharm Sci 2012;4:72-8.
16. Badami S, Vijayan P, Mathew N, Chandrashekhar R, Godavarthi A, Dhanaraj SA, et al. In vitro cytotoxic properties of grewia tiliaefoliabark and lupeol. Indian J Pharm 2003;35:250-1.
17. Anjaneyulu B, Baburao V, Ganguly AK, Govindachari TR, Joshi BS, Kamat VN, et al. Chemical investigation of some Indian plants. Indian J Chem 1965;3:237-8.
18. Badami S, Gupta MK, Ramaswami S, Rai SR, Najanan M, Bendell DJ, et al. Determination of betulin in grewia tiliaefolia by HPTLC. J Sep Sci 2004;27:129.
19. Iqbal Ahmad, Farrukh Aqil. In vitro efficacy of bioactive extracts of 15 medicinal plants against EsbLproducing multidrug-resistant enteric bacteria. Microbiol Res 2007;162:264-75.
20. Kareru PG, Keriko Gachanja JM, Kenji AN. Direct detection of triterpenoid saponins in medicinal plants. Afr J Tradit Complementary Altern Med 2008;5:56-60.
21. Sachin Shridhar Sakat, Archana Ramesh Juvekar. Analgesic and antipyretic activity of aqueous extract of grewia tiliaefolia Vahl leaves. J Pharm Res 2009;2:1475-8.
22. Selvam N Thamizh, Vengatakrishnan V. Evaluation of tissue level antioxidant activity of premna serratifolia leaf in paracetamol intoxicated wistar albino rats. Int J Pharm Life Sci 2010;1:86-90.
23. Grube M, Muter O, Strikauska S, Gavare M, Limane B. Application of FTIR spectroscopy for control of the medium composition during the biodegradation of nitro aromatic compounds. J Indian Microbiol Biotechnol 2008;35:1545–9.
24. Harborne JB. Methods of plants analysis: In: phytochemical methods. 2nd Eds. UK: Chapman and hall; 1984.
25. Trease GE, Evans WC. Introduction and general methods in pharmacognosy. 13th Eds. K: Cambridge University Press; 1989.
26. Raaman N. Phytochemical techniques, New India publishing agency, New Delhi; 2006.
27. Maria Lysete, Bastos A, Maria Raquel, Lima F, Lucia M, Conserva. Studies on the antimicrobial activity and brine shrimp toxicity of Zeyheria tuberculosa (Vell.) Bur. (Bignoniaceae) extracts and their main constituents. Ann Clin Microb Antimic 2009;8:16.
28. Meyer JJM, Afolayan AJ, Taylor MB, Erasmus D. Antiviral activity of galangin isolated from the aerial parts of Helichrysum aureonitens. J Ethnopharmacol 1997;56:165-9.
29. Maobe MAG, Nyarango RM. Fourier transformer infra red spectrophotometer analysis of warburgia ugandensis medicinal herb used for the treatment of diabetes, malaria and pneumonia in kisii region, southwest kenya. Global J Pharmacol 2013;7:61-8.
Published
15-07-2019
How to Cite
DEVI, D. R., and G. R. BATTU. “QUALITATIVE PHYTOCHEMICAL SCREENING AND FTIR SPECTROSCOPIC ANALYSIS OF GREWIA TILIFOLIA (VAHL) LEAF EXTRACTS”. International Journal of Current Pharmaceutical Research, vol. 11, no. 4, July 2019, pp. 100-7, doi:10.22159/ijcpr.2019v11i4.34936.
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