IN VITRO ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANTS TURMERIC, CHINNAMON, AND CLOVE AGAINST GM (+VE) AND GM (-VE) BACTERIA
DOI:
https://doi.org/10.22159/ijcpr.2019v11i5.35712Keywords:
Medicinal plants, Turmeric, Cinnamon, Clove, Bacteria, Antibiotic resistanceAbstract
Objective: Various diseases are caused by different pathogenic microorganisms. Antibiotics are being used for treatment of these infectious diseases, yet unpredictable utilization of it leads towards antibacterial resistance. It is required to discover better approaches to battle against antibacterial resistance. Therefore, the study aimed to detect antibacterial sensitivity of ethanol extracts of Curcuma longa (turmeric), Cinnamomumzeylanicum (clove) and Syzygiumaromaticum (cinnamon) against Syaphylococcus aureus and E coli.
Methods: Prior to sensitivity testing, ethanol oils were extracted by an electric blender and each of the bacteria strains were cultured onto blood agar plate. Antibacterial activity was tested by agar well diffusion method where three different concentrations (50 μl, 75 μl and 100 μl) of selected plants extract were used so far as to measure the inhibition zone. Inhibition zone of the ethanol extract of these plants were calculated where three were found to be sensitive against Syaphylococcus aureus and E coli.
Results: Greater inhibition zone 14.5 mm, 18.25 mm, 21.5 mm at100 μl against Syaphylococcus aureus in case of cinnamon whereas the least inhibition zone was showed by turmeric and it was 9.00 mm, 11.00 mm, and 12.75 mm at 100 μl extract against E. coli.
Conclusion: Overall, all the ethanol extracts were found to effective against these two bacteria but cinnamon can be used as more effective antibacterial agent in both human and veterinary field after the toxicological test.
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References
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2. Islam MA, Hossain MN, Chokraborti SS, Rahman S, Tasnim A, Al Zabir A. Socio-economic profile of goat rearing farmers and their management practices in sylhet, Bangladesh. J Agric Ecol Res Int 2018;1:1-0.
3. Chokraborti SS, Baset MA, Al Zabir A, Islam MA. Are Dairy Farmers of Haor (Wetland) Areas Food Secured? Asian J Agric Extension Econ Sociol 2019;29:1-8.
4. Ahmed S, Uddin MJ, Islam MA, Haque ME. Effect of Graded Levels of Slaughter House Residues on Growth Performance and Haematological Parameters in Broiler Chicken’s Ration. Asian Res J Agric 2018;9:1-8.
5. Schmidt B, Ribnicky DM, Poulev A, Logendra S, Cefalu WT, Raskin I. A natural history of botanical therapeutics. Metabolism 2008;57:S3-9.
6. Zakaria M. Isolation and characterization of active compounds from medicinal plants. Asia Pacific J Pharmacol 1991;6:15-20.
7. Mahady GB. Medicinal plants for the prevention and treatment of bacterial infections. Curr Pharm Design 2005;11:2405-27.
8. Kalemba DA, Kunicka A. Antibacterial and antifungal properties of essential oils. Curr Med Chem 2003;10:813-29.
9. Rates SM. Plants as a source of drugs. Toxicon 2001;39:603-13.
10. Subapriya R, Nagini S. Medicinal properties of neem leaves: a review. Curr Med Chem Anti-Cancer Agents 2005;5:149-56.
11. Farnsworth NR. NAPRALERT database. University of Illinois at Chicago: Chicago, IL, production (an online database available directly through the University of Illinois at Chicago or through the Scientific and Technical Network [STN] of Chemical Abstracts Services); 2004.
12. Martin KW, Ernst E. Herbal medicines for the treatment of bacterial infections: a review of controlled clinical trials. J Antimicrob Chemother 2003;51:241-6.
13. Clavero MR, Monk JD, Beuchat LR, Doyle MP, Brackett RE. Inactivation of escherichia coli O157:H7, salmonellae, and campylobacter jejuni in raw ground beef by gamma irradiation. Appl Environ Microbiol 1994;60:2069-75.
14. Beuchat LR, Golden DA. Antimicrobials occurring naturally in foods. Food Technology (USA). 1989;43:134-42.
15. Negi PS, Jayaprakasha GK, Jagan Mohan Rao L, Sakariah KK. Antibacterial activity of turmeric oil: a byproduct from curcumin manufacture. J Agric Food Chem 1999;47:4297-300.
16. Rudrappa T, Bais HP. Curcumin, a known phenolic from Curcuma longa, attenuates the virulence of Pseudomonas aeruginosa PAO1 in whole plant and animal pathogenicity models. J Agric Food Chem 2008;56:1955-62.
17. Kim KJ, Yu HH, Cha JD, Seo SJ, Choi NY, You YO. Antibacterial activity of curcuma longa L. against methicillin?resistant staphylococcus aureus. Phytother Res: Int J Devoted Pharmacol Toxicol Evaluation Natural Product Derivatives 2005;19:599-604.
18. Yuste J, Fung DY. Evaluation of salmonella typhimurium, yersinia enterocolitica and staphylococcus aureus counts in apple juice with cinnamon, by conventional media and thin agar layer method. Food Microbiol 2003;20:365-70.
19. Friedman M, Henika PR, Mandrell RE. Bactericidal activities of plant essential oils and some of their isolated constituents against campylobacter jejuni, Escherichia coli, Listeria monocytogenes, and Salmonella enterica. J Food Protection 2002;65:1545-60.
20. Chao SC, Young DG, Oberg CJ. Screening for inhibitory activity of essential oils on selected bacteria, fungi and viruses. J Essential Oil Res 2000;12:639-49.
21. Gutierrez J, Barry Ryan C, Bourke P. The antimicrobial efficacy of plant essential oil combinations and interactions with food ingredients. Int J Food Microbiol 2008;124:91-7.
22. Jantan IB, Karim Moharam BA, Santhanam J, Jamal JA. Correlation between chemical composition and antifungal activity of the essential oils of eight cinnamomum. Species Pharm Biol 2008;46:406-12.
23. Shahverdi AR, Monsef Esfahani HR, Tavasoli F, Zaheri A, Mirjani R. Trans?cinnamaldehyde from cinnamomumzeylanicum bark essential oil reduces the clindamycin resistance of clostridium difficile in vitro. J Food Sci 2007;72:S055-8.
24. Shan B, Cai YZ, Brooks JD, Corke H. Antibacterial properties and major bioactive components of cinnamon stick (Cinnamomumburmannii): activity against foodborne pathogenic bacteria. J Agric Food Chem 2007;55:5484-90.
25. Helander IM, Alakomi HL, Latva Kala K, Mattila Sandholm T, Pol I, Smid EJ, et al. Characterization of the action of selected essential oil components on gram-negative bacteria. J Agric Food Chem 1998;46:3590-5.
26. Di Pasqua R, Betts G, Hoskins N, Edwards M, Ercolini D, Mauriello G. Membrane toxicity of antimicrobial compounds from essential oils. J Agric Food Chem 2007;55:4863-70.
27. Nascimento GG, Locatelli J, Freitas PC, Silva GL. Antibacterial activity of plant extracts and phytochemicals on antibiotic-resistant bacteria. Brazilian J Microbiol 2000;31:247-56.
28. Saxena G, McCutcheon AR, Farmer S, Towers GH, Hancock RE. Antimicrobial constituents of Rhusglabra. J Ethnopharmacol 1994;42:95-9.
29. Bisset NG. Herbal drugs and phytopharmaceuticals: a handbook for practice on a scientific basis. Medpharm Scientific Publishers; 1994. p. 704.
30. Carvalho V, Melo VM, Aguiar A, Matos FS. Toxicity evaluation of medicinal plant extracts by the brine shrump (Arthenussalina Leah) biossay. Cienciae Cultura 1988;40:1109-11.
31. Chandler RF, Hooper SN, Harvey MJ. Ethnobotany and phytochemistry of yarrow, Achilleamillefolium, Compositae. Economic Bot 1982;36:203-23.
Published
15-09-2019
How to Cite
MD ARIFUL ISLAM, M. S. ISLAM, M. A. HOSSAIN, S. AHMED, M. AKTARUZZAMAN, M. R. AKANDA, M. M. HASAN TALHA, and A. AL ZABIR. “IN VITRO ANTIBACTERIAL ACTIVITY OF MEDICINAL PLANTS TURMERIC, CHINNAMON, AND CLOVE AGAINST GM (+VE) AND GM (-VE) BACTERIA”. International Journal of Current Pharmaceutical Research, vol. 11, no. 5, Sept. 2019, pp. 85-88, doi:10.22159/ijcpr.2019v11i5.35712.
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