EVALUATION OF BIOACTIVE COMPONENTS AND ANTIOXIDANT ACTIVITY OF VERNONIA ELAEAGNIFOLIA DC. (ASTERACEAE) IN GLYCOPHYTIC AND HALOPHYTIC CONDITIONS
DOI:
https://doi.org/10.22159/ijcpr.2020v12i2.37490Keywords:
Vernonia elaeagnifolia, Asteraceae, GC-MS, Antioxidants, Glycophytic, HalophyticAbstract
Objective: The investigate some of the antioxidant activities and bioactive potentials of Vernonia elaeagnifolia grown hydroponically in glycophytic and halophytic conditions.
Methods: Plants were grown in Hoagland solution and subjected to (Sodium chloride (NaCl) treatments. Plants cultivated without salt stress served as the control. The phytochemical analysis was done with the help of Gas Chromatography-Mass Spectrometry (GC-MS) and antioxidants were analyzed following standard procedures using UV-Visible Spectrophotometer.
Results: Significant changes were observed in the presence of antioxidants like superoxide dismutase (SOD), catalase (CAT), guaiacol peroxidase (GPX), ascorbate peroxidase (APX), total polyphenols, proline and ascorbic acid. GPX and proline proved to be the major components in imparting salinity stress tolerance. The control showed the presence of 23 bioactive components while the treatment contained 19 components.
Conclusion: The study thus provides information regarding the place of collection of the particular plant. Glycophytic conditions prove to be the best choice in terms of bioactive components and fatty acid contents. The investigations reveal that the plant contains several bioactive components and antioxidants, which prove it as a promising plant for developing potential drugs.
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2. Rao PM, Alberico GL, Cramer GR. Is the salt tolerance of maize related to sodium exclusion? Preliminary screening of seven cultivars. J Plant Nutr 2012;16:2289-303.
3. Al-Khanjari S, Al-Kathiri A, Esechie HA. Variation in chlorophyll meter readings, nodulation and dry matter yields of alfalfa (Medicago sativa L.) cultivars differing in salt tolerance. Crop Res 2002;24:350-6.
4. Samuel JK, Andrews B. Traditional medicinal plant wealth of pachalur and periyur hamlets, Dindigul District, Tamilnadu. Indian J Traditional Knowledge 2010;9:264-70.
5. Vongsombath C, de Boer HJ, Palsson K. Keeping leeches at bay: field evaluation of plant-derived extracts against terrestrial bloodsucking leeches (Haemadipsidae) in Lao PDR. Acta Trop 2011;119:178-82.
6. Epstein E. Mineral nutrition of plants: principles and perspectives. John Wiley and Sons, New York; 1972.
7. Taiz L, Zeiger E. Plant physiology: (III rd Edition) Sinauer Associates. Inc. Publishers, Sunderland, Massachussetts; 2002.
8. Yin D, Chen S, Chen F. Morphological and physiological responses of two Chrysanthemum cultivars differing in their tolerance to waterlogging. Environ Exp Bot 2009;67:87–93.
9. Aebi H. Catalase in vitro. Methods Enzymol 1984;105:121-6.
10. Giannopolitis CN, Reis SK. Superoxide dismutase I. Occurrence in higher plants. Plant Physiol 1997;59:309–14.
11. Chance B, Maehly AC. Assay of catalase and peroxidase. Methods Enzymol 1955;2:764-5.
12. Nakano Y, Asada K. Hydrogen peroxide is scavenged by ascorbate specific peroxidase in spinach chloroplasts. Plant Cell Physiol 1981;22:867-80.
13. Mukherjee SP, Choudhari MA. Implications of water stress-induced changes in the levels of endogenous ascorbic acid and hydrogen peroxide in Vigna seedlings. Physiol Plant 1983;58:166-70.
14. Bates LS, Waldern RP, Teare ID. Rapid determination of free proline for water-stress studies. Plant Soil 1973;39:205-8.
15. Makkar HPS. Measurement of total phenolics and tannins using the folin-ciocalteu method. Quantification of Tannins in Tree and Shrub Foliage. Springer, Dordrecht; 2003.
16. Thatoi N, Patra JK, Das SK. Free radical scavenging and antioxidant potential of mangrove plants: a review. Acta Physiol Plant 2014;36:31-42.
17. Takemura T, Hanagata N, Sugihara K, Baba S, Karube I, Dubinsky Z. Physiological and biochemical responses to salt stress in the mangrove, Bruguiera gymnorrhiza. Aquat Bot 2000;68:15–28.
18. Parida AK, Das AB, Mohanty P. Invesigations on the antioxidative defence response to NaCl stress in a mangrove, Bruguiera parviflora: differential regulations of isoforms of some antioxidative enzymes. Plant Growth Regul 2004;42:213–26.
19. Agastian P, Kingsley SJ, Vivekanandan M. Effect of salinity on photosynthesis and biochemical characteristics in mulberry genotypes. Photosynthetica 2000;38:287–90.
20. Mittler R. Oxidative stress, antioxidants and stress tolerance. Trends Plant Sci 2002;7:405-10.
21. Kumar PP, Kumaravel S, Lalitha C. Screening of antioxidant activity, total phenolics and GC-MS study of Vitex negundo. Afr J Biochem Res 2010;4:191-5.
22. Ogunlesi M, Okiei W, Osibote EA. Analysis of the essential oil from the leaves of Sesamum raditum, a potential medication for male infertility factor, by gas chromatography-mass spectrometry. Afr J Biotech 2010;9:1060-7.
23. Li RW, Leach DN, Myers P, Leach GJ, Lin GD, Brushett DJ, et al. Anti-inflammatory activity, cytotoxicity and active compounds of Tinospora smilacina. Benth Phytother Res 2004;18:78-83.
24. Chen L, Su J, Li L, Li B, Li W. A new source of natural D-borneol and its characteristic. J Med Plants Res 2011;5:3440-7.
25. Patel SS, Savjani JK. Systematic review of plant steroids as potential anti-inflammatory agents: current status and future perspectives. J Phytopharmacol 2015;4:121-5.
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