ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR
DOI:
https://doi.org/10.22159/ijcpr.2020v12i2.37495Keywords:
Baccharislatifolia, Natural extracts, Antimicrobial, AntioxidantAbstract
Objective: The increase in chronic and degenerative diseases and the use of synthetic antioxidants such as (butylated hydroxyanisole (BHA) or butylated hydroxytoluene (BHT)) are being restricted because they can be considered carcinogenic. Therefore, there is a growing interest in the search for natural antioxidants, especially from plants, due to their content in different bioactive compounds, such as antioxidants and antimicrobials. To evaluate the antibacterial and antioxidant activity of Baccharislatifolia extracts.
Methods: For the determination of the antimicrobial activity of extracts of leaves, root, stem and flowers of Baccharislatifolia (Bl), the disk plate diffusion method was used, the strains of Listeria, Salmonella and E. coli were studied; antibiotics Penicillin G and Ciprofloxacin were the controls. For the antioxidant activity, a solution of H2O2 (Abs at 230 nm) was prepared in Potassium Phosphate Monobasic-Sodium Hydroxide buffer.
Results: The antimicrobial activity against Listeria and Salmonella, showed that the extracts of leaves and flowers were more effective with inhibition zones>15 mm and>20 mm respectively. In front of E. coli, the extracts of flowers and stem were the best with zones>7.0 mm. Antibiotics studied inhibited the development of Listeria and Salmonella. However, E. coli isolates were resistant. In the antioxidant activity, the flower extract of Bl in 60 mg/ml presents a higher effect with 47.25%.
Conclusion: Bl extracts from leaves and flowers were more efficient both in their antimicrobial and antioxidant capacity.
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References
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26. Odedina GF, Vongkamjan K, Voravuthikunchai SP. Use of Rhodomyrtustomentosa ethanolic leaf extract for the bio-control of Listeria monocytogenes post-cooking contamination in cooked chicken meat. J Food Sci Technol 2016:53:4234–43.
27. Carrizo R, Ponzi M, Ardanaz C, Tonn CE, Donadel O. Chemical composition of essential oil of Baccharissalicifolia (RUIZ and PAVON) pers and antibacterial activity. J Chilean Chem Soc 2009;54:475-6.
28. Shan B, Cai Y, Brooks J, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbiol 2007;117:112-9.
29. Boziaris IS, Proestos C, Kapsokefalou M, Komaitis M. Antimicrobial effect of Filipendulaulmaria plant extract against selected foodborne pathogenic and spoilage bacteria in laboratory media, fish flesh and fish roe product. Food Technol Biotechnol 2011;49:263–70.
30. N'guessan JD, Coulibaly A, Ramanou AA, Okou OC, Djaman AJ, Guede Guina F. Antibacterial activity of thonningiasanguinea against some multi-drug resistant strains of Salmonella enteric. Afr Health Sci 2007;7:155–8.
31. Lee MH, Kwon HA, Kwon DY, Park H, Sohn DH, Kim YC, et al. Antibacterial activity of medicinal herb extracts against Salmonella. Int J Food Microbiol 2006;111:270–5.
32. Kamba AS, Hassan LG. Phytochemical screening and antimicrobial activities of Euphorbia balasamifera leaves stems and root against some pathogenic microorganisms. Afr J Pharm Sci Pharm 2010;1:57-64.
33. Bayas Morejon F, Tigre Leon A, Ruilova M, Ramon R. Actividad antibacteriana de extractos de Physalis peruviana L. contra cepas de Salmonellaspp. aisladas de carnes. XXI Congreso Nacional de Microbiología de los Alimentos Sesion 3A Productos Carnicos; 2018. p. 172-3.
34. Bachir Raho, Benali. Antibacterial activity of the essential oils from the leaves of Eucalyptus globules against Escherichia coli and Staphylococus aureus. Asian Pacific J Trop Biomed 2012;2:739-42.
35. Argote Vega F, Suarez Montenegro Z, Tobar Delgado M, Perez Alvarez J, Hurtado Benavides A, Delgado Ospina J. Evaluación de la capacidad inhibitoria de aceites esenciales en Staphylococcusaureus y Escherichia coli. Biotecnología en el Sector Agropecuario y Agroindustrial. Edición Especial No 2 • julio–diciembre; 2017. p. 52-60.
36. Bastos Oyarzabal M, Dame Schuch L, de Souza L, Almeida D, Alves Rodriguez M, Braga de Mello J. Actividad antimicrobiana de aceite esencial de Origanumvulgare L. ante bacterias aisladas en leche de bovino. Rev Cubana Plant Med 2011;16:260-6.
37. Prada J, Orduz Diaz L, Coy Barrera E. Baccharislatifolia: a lowly-valued asteraceous plant with chemical and medicinal potential in neotropics. Revista Facultad de Ciencias Básicas, Universidad Militar Nueva Granada 2016;12:92-105.
38. Martinez S, Terraza E, Alvarez T, Manani O, Vila J, Mollinedo P. Actividad antifungica in vitro de extractos polares de plantas del genero baccharis sobre fitopatogenos. Rev Boliv Quim 2010;27:13-8.
39. Abad M, Bermejo P. Baccharis (Compositae): a review update. ARKIVOC 2007;7:76-96.
40. Ramadan M, El-Ghorab A, Ghanem K. Volatile compounds, antioxidants, and anticancer activities of cape gooseberry fruit (Physalis peruviana L.): an in vitro study. J Arab Soc Med Res 2015;10:56-64.
41. Hara Kudo Y, Kobayashi A, Sugita Konishi Y, Kondo K. Antibacterial activity of plants used in cooking for aroma and taste. J Food Protection 2004;67:2820–4.
42. Guerra P. Evaluación de la actividad antioxidante bioautografica de dos variedades de aceites esenciales andinos Clinopodiumnubigenum (Kunt) Kuntze y Baccharis latifolia (Ruiz andPav.) Pers. trabajo de titulacion previo a la obtención del titulo de: Ingeniero en biotecnologia de los recursos naturales, Universidad Politecnica Salesiana 2016. p. 80.
43. Baljeet SY, Roshanlal Y, Ritika BY. Effect of cooking methods and extraction solvents on the antioxidant activity of summer squash (Cucurbita pepo) vegetable extracts. Int Food Res J 2016;23:1531-40.
44. Hossain SJ, Sultana S, Abu Taleb M, Basar M, Sarower M, Hossain SK. Antioxidant activity of ethanol and lipophilic extracts of common fruity vegetables in Bangladesh. Int J Food Properties 2014;17:2089-99.
45. Doroteo VH, Diaz C, Terry C, Rojas R. Compuestos fenolicos y actividad antioxidante in vitro de 6 plantas peruanas. Rev Soc Quim Peru 2013;79:13-20.
46. Rodriguez O, Andrade W, Diaz F. Antioxidant activity of extracts from leaves of Bocconiafrutescens L. (Papaveraceae). J Technol 2015;14:21-36.
2. Flores JM, Ochoa Zaragoza M, Lopez L, Trejo E, Morelos A. Drug interactions related to the administration of beta-lactam antibiotics. Revista ADM 2016;73:227-34.
3. Olaimat A, Al-Holy MA, Shahbaz H, Al-Nabulsi H, Abu Ghoush MH, Osaili T, et al. Emergence of antibiotic resistance in listeria monocytogenes isolated from food products: a comprehensive review. Compr Rev Food Sci Food Saf 2018;17:1277-92.
4. Tadesse G, Tessema T, Beyene G, Aseffa A. Molecular epidemiology of fluoroquinolone-resistant Salmonella in Africa: a systematic review and meta-analysis. PLoS One 2018;13:e0192575.
5. Shakti R, Rabindra N. Prevalence of fluoroquinolone resistance in Escherichia coli in an Indian teaching hospital and adjoining communities. J Taibah University Med Sci 2015;10:504-8.
6. Alos J. Resistencia bacteriana a los antibióticos: una crisis global. Enferm Infecc Microbiol Clin 2015;33:692-9.
7. Gadea R, Glibota NP, Perez R, Galvez A, Orteg AE, Fernandez M, et al. Effects of exposure to quaternary-ammonium-based biocides on antimicrobial susceptibility and tolerance to physical stresses in bacteria from organic foods. Food Microbiol 2017;63:58-71.
8. Mostafa AA, Al-Askar A, Almaary KS, Dawoud TM, Sholkamy EN, Bakri MM. Antimicrobial activity of some plant extracts against bacterial strains causing food poisoning diseases. Saudi J Biol Sci 2018;25:361–6.
9. Elisha IL, Botha FS, McGaw LJ, Eloff JN. The antibacterial activity of extracts of nine plant species with good activity against Escherichia coli against five other bacteria and cytotoxicity of extracts. BMC Complementary Alternative Medicine 2017;17: 133.
10. Devi W, Singh SB, Singh CB. Antioxidant and anti-dermatophytic properties leaf and stem bark of Xylosmalongi foliumclos. BMC Complement Altern Med 2013;13:155.
11. Ahmad R. Free radicals, antioxidants and diseases, Chapter 1. Basics of Free Radicals and Antioxidants. Intech Open 2018;1-5. http://dx.doi.org/10.5772/intechopen.76689.
12. Mateos R, Bravo L. Chromatographic and electrophoretic methods for the analysis of biomarkers of oxidative damage to a macromolecule (DNA, lipids and proteins). J Separation Sci 2007;30:175-91.
13. Atmani D, Ruiz Larrea MB, Ruiz Sanz JI, Lizcano LJ, Bakkali F, Atmani D. Antioxidant potential, cytotoxic activity and phenolic content of Clematis flammula leaf extracts. J Med Plants Res 2011;5:589.
14. Valarezo E, Rosillo M, Cartuche L, Malagon O, Meneses M, Morocho V. Chemical composition, antifungal and antibacterial activity of the essential oil from Baccharislatifolia (Ruiz and Pav.) Pers. (Asteraceae) from Loja, Ecuador. J Essential Oil Res 2013;25:233-8.
15. Salcedo Ortiz L, Almanza Vega G. Uso de Baccharis latifolia (Chilca) en La Paz, Bolivia. Biofarbo 2011;19:59-63.
16. Sequeda L, Ramos V, Monroy EC, Matulevich J, Tellez AN, Luengas PE. Actividad microbiana de Baccharis latifolia (Ruiz and Pavón) Pers, (Asteraceae) sobre microorganismos patogenos y cariogénicos. V Congreso Iberoamericano de Productos Naturales XIII Congreso Colombiano de Fitoquimica VIII Congreso Colombiano de Cromatografía, Sociedad Colombiana de Ciencias Químicas 2016;BA-24. Doi:10.13140/RG.2.1.3721.3047.
17. Shokeen P, Bala M, Tandon V. Evaluation of the activity of 16 medicinal plants against neisseriagonorrhoeae. Int J Antimicrobial Agents 2009;33:86-91.
18. Ruilova Cueva M, Tigre Leon RA, Lopez M, Yanchaliquin A, Bayas Morejon F, Sanaguano H. Antibacterial effects of uvilla (Physalis peruviana L.) stractsagaints Listeria spp. Isolated from meat in Ecuador. Int J Curr Microbiol Appl Sci 2017;6:1146-53.
19. Ponce A, Roura S, Del Valle C, Moreira M. Antimicrobial and antioxidant activities of edible coatings enriched with natural plant extracts: in vitro and in vivo studies. Postharvest Biol Technol 2008;49:294-300.
20. Ruch R, Cheng SJ, Klaunig JE. Prevention of cytotoxicity and inhibition of intracelular communication by antioxidant catechins isolated from Chinese green tea. Carcinogenesis 1989;10:1003-8.
21. Perez Jimenez J. Metodologia para la evaluacion de ingredientes funcionales antioxidantes efecto de fibra antioxidante de uva en status antioxidante y parámetros de riesgo cardiovascular en humanos. Tesis Doctoral Universidad Autonoma de Madrid; 2007. p. 272.
22. CLSI. Manual de actualización en resistencia bacteriana y normas CLSI M100-S20. Grebo. Capitulo 2012;3:6-8.
23. Preston S, Drusano G. Penicillins infectious disease antimicrobial agentes. Antimicrobe 2008;32:373
24. Yoon Y, Choi KH. Antimicrobial activities of therapeutic herbal plants against Listeria monocytogenes and the herbal plant cytotoxicity on caco-2 cell. Lett Appl Microbiol 2013;55:47–55.
25. Eruteya O, Badon B. Antilisterial activity of ethanolic and aqueous extracts of the leaves and seeds of Moringa oleifera. Asia J Appl Microbiol 2014;1:60-5.
26. Odedina GF, Vongkamjan K, Voravuthikunchai SP. Use of Rhodomyrtustomentosa ethanolic leaf extract for the bio-control of Listeria monocytogenes post-cooking contamination in cooked chicken meat. J Food Sci Technol 2016:53:4234–43.
27. Carrizo R, Ponzi M, Ardanaz C, Tonn CE, Donadel O. Chemical composition of essential oil of Baccharissalicifolia (RUIZ and PAVON) pers and antibacterial activity. J Chilean Chem Soc 2009;54:475-6.
28. Shan B, Cai Y, Brooks J, Corke H. The in vitro antibacterial activity of dietary spice and medicinal herb extracts. Int J Food Microbiol 2007;117:112-9.
29. Boziaris IS, Proestos C, Kapsokefalou M, Komaitis M. Antimicrobial effect of Filipendulaulmaria plant extract against selected foodborne pathogenic and spoilage bacteria in laboratory media, fish flesh and fish roe product. Food Technol Biotechnol 2011;49:263–70.
30. N'guessan JD, Coulibaly A, Ramanou AA, Okou OC, Djaman AJ, Guede Guina F. Antibacterial activity of thonningiasanguinea against some multi-drug resistant strains of Salmonella enteric. Afr Health Sci 2007;7:155–8.
31. Lee MH, Kwon HA, Kwon DY, Park H, Sohn DH, Kim YC, et al. Antibacterial activity of medicinal herb extracts against Salmonella. Int J Food Microbiol 2006;111:270–5.
32. Kamba AS, Hassan LG. Phytochemical screening and antimicrobial activities of Euphorbia balasamifera leaves stems and root against some pathogenic microorganisms. Afr J Pharm Sci Pharm 2010;1:57-64.
33. Bayas Morejon F, Tigre Leon A, Ruilova M, Ramon R. Actividad antibacteriana de extractos de Physalis peruviana L. contra cepas de Salmonellaspp. aisladas de carnes. XXI Congreso Nacional de Microbiología de los Alimentos Sesion 3A Productos Carnicos; 2018. p. 172-3.
34. Bachir Raho, Benali. Antibacterial activity of the essential oils from the leaves of Eucalyptus globules against Escherichia coli and Staphylococus aureus. Asian Pacific J Trop Biomed 2012;2:739-42.
35. Argote Vega F, Suarez Montenegro Z, Tobar Delgado M, Perez Alvarez J, Hurtado Benavides A, Delgado Ospina J. Evaluación de la capacidad inhibitoria de aceites esenciales en Staphylococcusaureus y Escherichia coli. Biotecnología en el Sector Agropecuario y Agroindustrial. Edición Especial No 2 • julio–diciembre; 2017. p. 52-60.
36. Bastos Oyarzabal M, Dame Schuch L, de Souza L, Almeida D, Alves Rodriguez M, Braga de Mello J. Actividad antimicrobiana de aceite esencial de Origanumvulgare L. ante bacterias aisladas en leche de bovino. Rev Cubana Plant Med 2011;16:260-6.
37. Prada J, Orduz Diaz L, Coy Barrera E. Baccharislatifolia: a lowly-valued asteraceous plant with chemical and medicinal potential in neotropics. Revista Facultad de Ciencias Básicas, Universidad Militar Nueva Granada 2016;12:92-105.
38. Martinez S, Terraza E, Alvarez T, Manani O, Vila J, Mollinedo P. Actividad antifungica in vitro de extractos polares de plantas del genero baccharis sobre fitopatogenos. Rev Boliv Quim 2010;27:13-8.
39. Abad M, Bermejo P. Baccharis (Compositae): a review update. ARKIVOC 2007;7:76-96.
40. Ramadan M, El-Ghorab A, Ghanem K. Volatile compounds, antioxidants, and anticancer activities of cape gooseberry fruit (Physalis peruviana L.): an in vitro study. J Arab Soc Med Res 2015;10:56-64.
41. Hara Kudo Y, Kobayashi A, Sugita Konishi Y, Kondo K. Antibacterial activity of plants used in cooking for aroma and taste. J Food Protection 2004;67:2820–4.
42. Guerra P. Evaluación de la actividad antioxidante bioautografica de dos variedades de aceites esenciales andinos Clinopodiumnubigenum (Kunt) Kuntze y Baccharis latifolia (Ruiz andPav.) Pers. trabajo de titulacion previo a la obtención del titulo de: Ingeniero en biotecnologia de los recursos naturales, Universidad Politecnica Salesiana 2016. p. 80.
43. Baljeet SY, Roshanlal Y, Ritika BY. Effect of cooking methods and extraction solvents on the antioxidant activity of summer squash (Cucurbita pepo) vegetable extracts. Int Food Res J 2016;23:1531-40.
44. Hossain SJ, Sultana S, Abu Taleb M, Basar M, Sarower M, Hossain SK. Antioxidant activity of ethanol and lipophilic extracts of common fruity vegetables in Bangladesh. Int J Food Properties 2014;17:2089-99.
45. Doroteo VH, Diaz C, Terry C, Rojas R. Compuestos fenolicos y actividad antioxidante in vitro de 6 plantas peruanas. Rev Soc Quim Peru 2013;79:13-20.
46. Rodriguez O, Andrade W, Diaz F. Antioxidant activity of extracts from leaves of Bocconiafrutescens L. (Papaveraceae). J Technol 2015;14:21-36.
Published
15-03-2020
How to Cite
BAYAS-MOREJON, F., . A. TIGRE, R. RAMON, and D. YANEZ. “ANTIMICROBIAL AND ANTIOXIDANT EFFECT OF NATURAL EXTRACTS FROM LEAVES, ROOT, STEM AND FLOWERS OF BACCHARIS LATIFOLIA FROM ECUADOR”. International Journal of Current Pharmaceutical Research, vol. 12, no. 2, Mar. 2020, pp. 78-84, doi:10.22159/ijcpr.2020v12i2.37495.
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