• SATHYA B. Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram 608002, Tamil Nadu, India
  • BALAMURUGAN K. Department of Pharmacy, Annamalai University, Annamalai Nagar, Chidambaram 608002, Tamil Nadu, India
  • ANBAZHAGAN S. Department of Pharmaceutical Chemistry, Surya School of Pharmacy, Vikravandi 605652, Villupuram, Tamil Nadu, India



Bioactive compounds, Anxiety, Diosgenin, Silymarin, Diazepam, Elevated plus-maze, Hole-board test, Light-Dark model


Objective: Evidence is emerging that specific combinations of bioactive compounds may be far more effective in protecting against several diseases as compared to the effect of a single compound. The present study was aimed to investigate the interactive effect of Diosgenin and Silymarin, the bioactive compounds from plant origin in an animal model of anxiety.

Methods: Albino wistar rats of either sex were divided into five groups and treated for 5 d. Group I and II served as control and standard and test groups were treated with Diosgenin (100 mg/kg, p. o.), Silymarin (100 mg/kg, p. o.) and combination of Diosgenin (50 mg/kg, p. o.)+Silymarin (50 mg/kg, p. o.), respectively. Diazepam (1 mg/kg, i. p.) was used as a standard for the study. Anxiolytic effects were studied in the Elevated plus-maze, Hole-board test and Light/Dark model.

Results: The results suggested that Diosgenin when given alone at a dose of 100 mg/kg, does not shown significant anxiolytic effect when as compared with control. Whereas, the compound Silymarin (100 mg/kg) shown significant anti-anxiety effect (P<0.01), independently. The same two bioactive compounds, given in combination at a dose of 50 mg/kg, (each), exhibited significant anxiolytic-like effect, potentially.

Conclusion: It can be concluded that Diosgenin has got a synergistic effect on anti-anxiety action when given in combination with Silymarin.


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How to Cite

B., S., B. K., and A. S. “COMBINED EFFECT OF SELECTIVE BIOACTIVE COMPOUNDS FROM PLANT ORIGIN IN AN ANIMAL MODEL OF ANXIETY”. International Journal of Current Pharmaceutical Research, vol. 12, no. 2, Mar. 2020, pp. 88-91, doi:10.22159/ijcpr.2020v12i2.37498.



Original Article(s)