• HAMZAH JUMAH ESSA BARKAH Department of Studies in Biotechnology, University of Mysore, Manasagongatri, Mysuru 57006, Karnataka, India
  • S. LOKESH Department of Studies in Biotechnology, University of Mysore, Manasagongatri, Mysuru 57006, Karnataka, India




Angiospermic epiphytes, Antifungal activity, Ergosterol, Electrolyte leakage, Pathogenic fungi


Objective: In the present study, emphasis has been made to assay the antifungal activity of some angiospermic epiphytes like Cuscuta reflexa, Viscum orientale, Cymbidium bicolor, Bulbophyllum propinquum, Hoya ovalifolia and Dendrophthoe falcata.

Methods: The antifungal activity due to epiphytes extracts was expressed in term of reduction in dry biomass of fungi based on the relationship of ergosterol content and electrolyte leakage in fungal pathogens viz., Colletotrichum dematium, Drechslera oryzae, Fusarium oxysporum and Fusarium solani treated with epiphytes extract.

Results: Treated fungi showed increased in ergostesrol content and increased electrolyte leakage confirmed the pathetic situation of fungi; hence there was fungistatic effect, which was dose-dependent.

Conclusion: The angiospermic epiphytes of selected species chosen for the study could be serves an alternative eco-friendly source to synthetic fungicides.


Download data is not yet available.


1. Gutarowska B, Zakowska Z. Mathematical models of mycelium growth and ergosterol synthesis in stationary mould culture. Lett Appl Microl 2009;5:605-10.
2. Cook N. The perception of large-scale tonal closure. Mus Perce: An Int J 1987;2:197-205.
3. West AW, Grant WD, Sparling GP. Use of ergosterol, diaminopimelic acid and glucosamine contents of soils to monitor changes in microbial populations. Soi Bio Bioch 1987;5:607-12.
4. Lumsden RD, Carter JP, Whipps JM, Lynch JM. Comparison of biomass and viable propagule measurements in the antagonism of Trichoderma harzianum against Pythium ultimum. Soi Biol Bioch 1990;2:187-94.
5. Martin F, Delaruelle C, Hilbert JL. An improved ergosterol assay to estimate fungal biomass in ectomycorrhizas. Mycol Res 1990;8:1059-64.
6. Gessner MO, Schwoerbel J. Fungal biomass associated with decaying leaf litter in a stream. Oecol 1991;4:602-3.
7. Gessner MO, Chauvet E. Ergosterol-to-biomass conversion factors for aquatic hyphomycetes. Appl Environ Microl 1993;2:502-7.
8. Suberkropp K, Gessner MO, Chauvet E. Comparison of ATP and ergosterol as indicators of fungal biomass associated with decomposing leaves in streams. Appl Environ Microl 1993;10:3367-72.
9. Sung SJS, White LM, Marx DH, Otrosina WJ. Seasonal ectomycorrhizal fungal biomass development on loblolly pine (Pinus taeda L.) seedlings. Mycology 1995;6:439-47.
10. Palta JP, Levitt J, Stadelmann EJ. Freezing injury in onion bulb cells: I. Evaluation of the conductivity method and analysis of ion and sugar efflux from injured cells. Plant Physiol 1977;3:393-7.
11. Bajji M, Kinet JM, Lutts S. The use of the electrolyte leakage method for assessing cell membrane stability as a water stress tolerance test in durum wheat. Plant Growth Regul 2002;1:61-70.
12. Demidchik V, Cuin TA, Svistunenko D, Smith SJ, Miller AJ, Shabala S, et al. Arabidopsis root K+-efflux conductance activated by hydroxyl radicals: single-channel properties, genetic basis and involvement in stress-induced cell death. J Cell Sci 2010;9:1468-79.
13. Zepeda Jazo I, Velarde Buendia AM, Enriquez Figueroa R, Bose J, Shabala S, Muniz Murguia J, et al. Polyamines interact with hydroxyl radicals in activating Ca2+and K+transport across the root epidermal plasma membranes. Plant Physiol 2011;4:2167-80.
14. Demidchik V, Straltsova D, Medvedev SS, Pozhvanov GA, Sokolik A, Yurin V. Stress-induced electrolyte leakage: the role of K+permeable channels and involvement in programmed cell death and metabolic adjustment. J Exp Bot 2014;5:1259-70.



How to Cite

BARKAH, H. J. E., and S. LOKESH. “EFFECT OF ANGIOSPERMIC EPIPHYTES ON THE RELATIVE STATUS OF ERGOSTEROL AND ELECTROLYTE LEAKAGE IN FUNGAL PATHOGENS”. International Journal of Current Pharmaceutical Research, vol. 12, no. 3, May 2020, pp. 111-3, doi:10.22159/ijcpr.2020v12i3.38317.



Original Article(s)