STUDY OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY BY MUSSAENDA MACROPHYLLA ROOT EXTRACTS

Authors

  • DEEPAK SHRESTHA Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • JITENDRA PANDEY Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • CHIRANJIBI GYAWALI Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • MAHESH LAMSAL Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • SUNITA SHARMA Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • RABINDRA KUMAR ROKAYA Department of Pharmacy, Karnali Academy of Health Science, Jumla, Nepal
  • PRAMOD ARYAL Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal, Department of Biochemistry and Molecular Biology, Monash University, Clayton, Australia
  • RAM BAHADUR KHADKA Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal
  • RAVIN BHANDARI Department of Pharmacy, Crimson College of Technology, Affiliated to Pokhara University, Devinagar-11, Butwal Nepal

DOI:

https://doi.org/10.22159/ijcpr.2020v12i4.39085

Keywords:

Mussaenda macrophylla, Anti-diabetic, Anti-oxidant, In vitro, DPPH

Abstract

Objective: The systematic study of effective alternative anti-diabetic drugs has great importance to manage diabetes as well as other oxidative stress-related diseases. According to previous research, root and bark of Mussaenda macrophylla plant has anti-microbial, anti-coagulant, anti-inflammatory, and hepatoprotective activity. Ethnomedicinal data shows that Mussaenda macrophylla is used to treat diabetes as well as oxidative stress. The objective of this research is to investigate in vitro anti-diabetic and anti-oxidant activity of root extract of Mussaenda macrophylla.

Methods: DPPH free radical scavenging assay was used to detect anti-oxidant potency of ethanol and methanol root extract of the plant and expressed as % of radicle inhibition. Anti-diabetic activity was determined by the glucose diffusion method using a glucose oxidase kit and results were expressed as mean±SD.

Results: The ethanol root extract at the concentration of 50 mg/ml and 100 mg/ml showed better glucose diffusion inhibition than that of methanol extract at the same concentration on increasing time interval. Ethanol extract at the concentration 100 µg/ml displayed better DPPH scavenging activity (89.83±0.19 %) than that of methanol extract (86.61±0.75%).

Conclusion: This study concluded that ethanol and methanol root extract of Mussenda macrophylla have potent anti-diabetic as well as anti-oxidant activity but further advance research is necessary in the animal model.

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Published

15-07-2020

How to Cite

SHRESTHA, D., J. PANDEY, C. GYAWALI, M. LAMSAL, S. SHARMA, R. K. ROKAYA, P. ARYAL, R. B. KHADKA, and R. BHANDARI. “STUDY OF IN VITRO ANTI-OXIDANT AND ANTI-DIABETIC ACTIVITY BY MUSSAENDA MACROPHYLLA ROOT EXTRACTS”. International Journal of Current Pharmaceutical Research, vol. 12, no. 4, July 2020, pp. 70-74, doi:10.22159/ijcpr.2020v12i4.39085.

Issue

Vol 12, Issue 4 (Jul-Aug), 2020

Section

Original Article(s)
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