IN VITRO EVALUATION OF IMMUNOMODULATORY ACTIVITY OF ETHANOLIC EXTRACT OF MORINGA CONCANENSIS NIMMO
DOI:
https://doi.org/10.22159/ijcpr.2020v12i4.39100Keywords:
Moringa concanensis, Ethanolic extract, Immunomodulatory, Plaque forming cellAbstract
Objective: The present study was undertaken to evaluate immunomodulatory activity of ethanolic extract and isolated compound from the whole plant of Moringa concanensis Nimmo
Methods: Immunomodulatory activities were determined by in vitro models-plaque-forming cell assay, nitric oxide (NO) radical scavenging activity, inhibit RAW 264.7 macrophage cell line from generating harmful NO induced by lipopolysaccharide (LPS) and MTT [3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] assay.
Results: Showed significant (P<0.01) phagocytic effect on human neutrophils in the parameters studied. Scavenged nitric oxide radical by ethanolic extract of Moringa concanensis inhibition 34% and isolated compound scavenged nitric oxide radical inhibition 44% respectively compared to control group exerted 52% decrease of nitric oxide radical respectively concentration at 0.1,0.01 and1.0 mg/ml. LPS in murine macrophage, RAW 264.7 cells culture medium induced a high release of NO but pretreatment with Moringa concanensis extract and isolated compound resulted in inhibition of NO production by 29, 37, 95 % and 32,44,96 % at concentrations of 0.5, 0.1 and 1.0 mg/ml, compared to LPS-induced sample. ethanolic extract and isolated compound significantly stimulate RAW 264.7 cell viability (P<0.001) by MTT assay.
Conclusion: The present experimental finding demonstrated that isolated compound has superior immunomodulatory activity than the ethanolic extract of Moringa concanensis this effect presumably due to the greater ability of an isolated compound to boost the innate and adaptive immune system.
Downloads
References
2. Alamgir M, Uddin SJ. Recent advances on the ethnomedicinal plants as immunomodulatory agents. Ethnomedicine: a source of complementary therapeutics. Debprasad Chattopadhyay 2010;695:227-44.
3. Kuttan G. Immunomodulatory effect of come naturally occurring sulfur-containing compounds. J Ethnopharmacol 2000;72:93-9.
4. Bodhankar S, Makare N, Rangari V. Immunomodulatory activity of alcoholic extract of Mangifera indica L. in mice. J Ethnopharmacol 2001;78:133-7.
5. Pezzuto JM. Plant-derived anticancer agents. Biochem Pharmacol 1997;53:121–33.
6. Diasio RB, LoBuglio AF. The pharmacological basis of therapeutics. In: Goodman, Gilman’s. editors. McGraw-Hill (NY); 1996. p. 1291–307.
7. Patil US, Jaydeokar V, Bandawane DD. Immunomodulators: a pharmacological review. Int J Pharm Pharm Sci 2012;4:30-6.
8. Anbazhakan S, Dhandapani R, Anandhakumar P, Balu S. Traditional medicinal knowledge on Moringa concanensis nimmo of perambalur district, Tamilnadu. Anc Sci Life 2007;26:42-5.
9. Ankita Dey, Bhattacharyya Sauryya, Pal Tapan Kumar. Antioxidant activities of moringa concanensis flowers (fresh and dried) grown in West Bengal. Int J Res Chem Environ 2014;4:64-70.
10. OECD: Guideline, 423, acute oral toxicity: Environmental Health and Safety Monograph series on Testing and Assessment; 2000.
11. Satish Verma K, Asima Shaban, Reena Purohit, Madhvi Chimata L, Geeta Rai, Om Prakash Verma. Immunomodulatory activity of Withania somnifera (L.). J Chem Pharm Res 2012;4:559-61.
12. Sneha J Anarthe, E Malavika, A Pravalika, M Ganga Raju. Screening of immunomodulatory activity of spahaeranthus idicus linn whole plant. Int J Phytomed 2015. p. 432-40.
13. Jin Won Chung, Jin Uk Oh, Sehyung Lee, Sung-Jin Kim. Inhibition of inducible nitric oxide synthase, cyclooxygenase-2, and lipid peroxidation by methanol extract of Pericarpium Zanthoxyli. Trop J Pharm Res 2013;12:369-75.
14. Guan D, Zhang Z, Yang Y, Xing G, Liu J. Immunomodulatory activity of polysaccharide from the roots of Actinidia kolomikta on macrophages. Int J Biol 2011;3:3–10.
15. Groesdonk HV, Schlottmann S, Richter F, Georgieff M, Senftleben U. Escherichia coli prevents phagocytosis-induced death of macrophages via classical NF-?B signaling, a link to T-cell activation. Infect Immun 2006;74:5989-6000.
16. Liyana Pathirana CM, Shahidi F. Antioxidant properties of commercial soft and hard winter wheats (Triticumaestivum L.) and their milling fractions. J Sci Food Agric 2006;86:477–85.
17. Yermilov V, Rubio J, Becchi M, Friesen MD, Pignatelli B, Ohshima H. Formation of 8-nitroguanidine by the reaction of guanine with peroxynitrite in vitro. Carcinogenesis 1995;16:2045–50.
Published
How to Cite
Issue
Section
