GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS IN ESSENTIAL OILS OF LEAF OF EUODIA SUAVEOLENS SCHEFF
DOI:
https://doi.org/10.22159/ijcpr.2020v12i4.39102Keywords:
GC-MS analysis, Euodia suaveolens, Bioactive compounds, Essential oilsAbstract
Objective: Essential oils extracted from the leaf of Euodia suaveolens have been reported. However, there is no published data on comprehensive report on the chemical constituents of the essential oils and the methods utilized to extract the essential oils from the plant. This research aimed to reveal and determine the chemical constituents of essential oils from the leaf of E. suaveolens.
Methods: Essential oils of leaf of E. suaveolens were extracted by steam distillation and were analyzed utilizing Gas Chromatography-Mass Spectrometry (GC-MS) methods.
Results: The GC-MS analysis revealed the presence of twenty-five different chemical constituents from the essential oils. The main chemical constituents of essential oils extracted from E. suaveolens’ leaves were as follows menthofuran (50.38 %), p-mentha-1,8-diene (14.34 %), limonen (10.99 %), evodone (5.55 %), α-curcumene (4.65 %), globulol (1.88 %), longipinenepoxide (1.66 %), and linalool (1.40 %). This present research found three compounds, namely p-mentha-1,8-diene, globulol, and longipinenepoxide that have never been reported by any researchers working with this plant.
Conclusion: The results showed that E. suaveolens contains essential oils that are potential to be explored further and utilized as medicinal products against some ailments.
Downloads
Download data is not yet available.
References
1. Djamal J. Euodia hortensis. JR forster, JG Forster. In: Lemmens RHMJ and Bunyapraphatsara N. (Editors). Plant Resources of South-East Asia, No. 12, Medicinal and Poisonous Plants 3, Backhuys Publishers, Leiden; 2003. p. 196-7.
2. Hassler M. World plants: synonymic checklists of the vascular plants of the world. In: Roskov Y, Ower G, Orrell T, Nicolson D, Bailly N, Kirk PM, Bourgoin T, DeWalt RE, Decock W, Nieukerken E van, Zarucchi J, Penev L. (Editors). Species 2000 and ITIS Catalogue of Life, Annual Checklist, Naturalis, Leiden, the Netherlands; 2019.
3. Simaremare EV, Sinaga DI, Agustini V. Sabun zodia sebagai Repellent terhadap nyamuk Aedes aegypti. Pharm J Indones 2017;3:11-6.
4. Lestari FD, Simaremare ES. Uji potensi minyak atsiri daun zodia (Evodia suaveolens Scheff.) sebagai insektisida nyamuk Aedes aegypti L dengan metode elektrik. Pharmacy 2017;14:1-10.
5. Mirawati P, Simaremare ES, Pratiwi RD. Uji efektivitas repellent sediaan lotion kombinasi minyak atsiri daun zodia (Evodia suaveolens Scheff.) dan minyak atsiri batang serai (Cymbopogon citratus) terhadap nyamuk Aedes aegypti L. Pharmacy 2018;15:1-15.
6. Widawati M, Santi M. The effectiveness of fixative addition on zodia (Evodia suaveolens S.) and rosemary (Rosmarinus officinalis L.) gel against Aedes aegypti. Health Sci Indones 2013;4:103-6.
7. Cameron RR, Arinafril, Mulawarman. Uji bioaktivitas ekstrak daun zodea (Evodia suaveolens Sheff) terhadap hama gudang Tribolium castaneum (Coleoptera: Tenebrionidae) herbst. E J Agroekoteknologi Tropika 2016;5:222-31.
8. Lestari MS, Himawan T, Abadi AL, Retnowati R. Toxicity and phytochemistry test of methanol extract of several plants from papua using Brine Shrimp Lethality Test (BSLT). J Chem Pharm Res 2015;7:866-72.
9. Chand R, Jokhan A, Gopalan R. Bioactivity of selected essential oils from medicinal plants found in Fiji against the spiralling whiteflies (Aleurodicus dispersus Russell). Adv Hort Sci 2017;30:165-74.
10. Maryuni AE. Isolasi dan identifikasi senyawa antibakteri minyak atsiri daun zodia (Evodia sp). Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor. Thesis; 2008.
11. Bozovic M, Navarra A, Garzoli S, Pepi F, Ragno R. Esential oils extraction: a 24 h steam distillation systematic methodology. Nat Prod Res 2017;31:2387-96.
12. Tisserand R, Young R. Essential oil profiles. http://dx.doi.org/10.1016/B978-0-443-06241-4.00013-8,2014.
13. Lahlou M. Methods to study the phytochemistry and bioactivity of essential oils. Phytother Res 2004;18:435–48.
14. Ung CY, White JML, White IR, Banerjee P, McFadden JP. Patch testing with the European baseline series fragrance markers. Br J Dermatol 2017;178:776-80.
15. Tan ML, Zhou LG, Huang YF, Wang Y, Hao XJ, Wang JG. Antimicrobial activity of globulol isolated from the fruits of Eucalyptus globulus Labill. J Nat Prod Res 2008;22:569-75.
16. Aggarwala BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharm 2006;71:1397-421.
17. Alviano WS, Mendonca Filho RR, Alviano DS, Bizzo HR, Souto Padron T, Rodrigues ML, et al. Antimicrobial activity of Croton cajucara benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. Oral Microbiol Immunol 2005;20:101–5.
18. Antonious GF, Kochhar TS. Zingiberene and curcumene in wild tomato. J Environ Sci Health Part B 2003;38:489-500.
19. Filly A, Fabiano Tixier AS, Louis C, Fernandez X, Chemat F. Water as a green solvent combined with different techniques for extraction of essential oil from lavender flowers. C R Chimie 2016;19:707-17.
20. Ozek G, Ozek T, Iscan G, Baser KHC, Hamzaoglu E, Duran A. Comparison of hydrodistillation and microdistillation methods for the analysis of fruit volatiles of Prangos pabularia lindl., and evaluation of its antimicrobial activity. South Afr J Bot 2007;73:563–9.
21. Amelia B, Saepudin E, Cahyana AH, Rahayu DU, Sulistyoningrum AS, Haib J. GC-MS analysis of clove (Syzygium aromaticum) bud essential oil from Java and Manado. AIP Conf Proc 2017;1862:030082.
22. Shettima AY, Karumi Y, Sodipo OA, Usman H, Tijjani MA. Gas chromatography-mass spectrometry (GC-MS) analysis of bioactive components of ethyl acetate root extract of Guiera senegalensis J F Gmel. J Appl Pharm Sci 2013;3:146-50.
23. Delazar A, Reid RG, Sarker SD. GC-MS analysis of essential oil of the oleoresin from Pistacia atlantica var mutica. Chem Nat Compounds 2004;40:24–7.
24. Brophy JJ, Rahmani M, Toia RF, Croft KD, Lassak EV. The volatile oils of Euodia hortensis forma hortensis. Flav Frag J 1985;1:17-20.
25. Handayani PA, Nurcahyanti H. Ekstraksi minyak atsiri daun zodia (Evodia suaveolens) dengan metode maserasi dan distilasi air. Jurnal Bahan Alam Terbarukan 2015;4:1-7.
2. Hassler M. World plants: synonymic checklists of the vascular plants of the world. In: Roskov Y, Ower G, Orrell T, Nicolson D, Bailly N, Kirk PM, Bourgoin T, DeWalt RE, Decock W, Nieukerken E van, Zarucchi J, Penev L. (Editors). Species 2000 and ITIS Catalogue of Life, Annual Checklist, Naturalis, Leiden, the Netherlands; 2019.
3. Simaremare EV, Sinaga DI, Agustini V. Sabun zodia sebagai Repellent terhadap nyamuk Aedes aegypti. Pharm J Indones 2017;3:11-6.
4. Lestari FD, Simaremare ES. Uji potensi minyak atsiri daun zodia (Evodia suaveolens Scheff.) sebagai insektisida nyamuk Aedes aegypti L dengan metode elektrik. Pharmacy 2017;14:1-10.
5. Mirawati P, Simaremare ES, Pratiwi RD. Uji efektivitas repellent sediaan lotion kombinasi minyak atsiri daun zodia (Evodia suaveolens Scheff.) dan minyak atsiri batang serai (Cymbopogon citratus) terhadap nyamuk Aedes aegypti L. Pharmacy 2018;15:1-15.
6. Widawati M, Santi M. The effectiveness of fixative addition on zodia (Evodia suaveolens S.) and rosemary (Rosmarinus officinalis L.) gel against Aedes aegypti. Health Sci Indones 2013;4:103-6.
7. Cameron RR, Arinafril, Mulawarman. Uji bioaktivitas ekstrak daun zodea (Evodia suaveolens Sheff) terhadap hama gudang Tribolium castaneum (Coleoptera: Tenebrionidae) herbst. E J Agroekoteknologi Tropika 2016;5:222-31.
8. Lestari MS, Himawan T, Abadi AL, Retnowati R. Toxicity and phytochemistry test of methanol extract of several plants from papua using Brine Shrimp Lethality Test (BSLT). J Chem Pharm Res 2015;7:866-72.
9. Chand R, Jokhan A, Gopalan R. Bioactivity of selected essential oils from medicinal plants found in Fiji against the spiralling whiteflies (Aleurodicus dispersus Russell). Adv Hort Sci 2017;30:165-74.
10. Maryuni AE. Isolasi dan identifikasi senyawa antibakteri minyak atsiri daun zodia (Evodia sp). Sekolah Pascasarjana, Institut Pertanian Bogor, Bogor. Thesis; 2008.
11. Bozovic M, Navarra A, Garzoli S, Pepi F, Ragno R. Esential oils extraction: a 24 h steam distillation systematic methodology. Nat Prod Res 2017;31:2387-96.
12. Tisserand R, Young R. Essential oil profiles. http://dx.doi.org/10.1016/B978-0-443-06241-4.00013-8,2014.
13. Lahlou M. Methods to study the phytochemistry and bioactivity of essential oils. Phytother Res 2004;18:435–48.
14. Ung CY, White JML, White IR, Banerjee P, McFadden JP. Patch testing with the European baseline series fragrance markers. Br J Dermatol 2017;178:776-80.
15. Tan ML, Zhou LG, Huang YF, Wang Y, Hao XJ, Wang JG. Antimicrobial activity of globulol isolated from the fruits of Eucalyptus globulus Labill. J Nat Prod Res 2008;22:569-75.
16. Aggarwala BB, Shishodia S. Molecular targets of dietary agents for prevention and therapy of cancer. Biochem Pharm 2006;71:1397-421.
17. Alviano WS, Mendonca Filho RR, Alviano DS, Bizzo HR, Souto Padron T, Rodrigues ML, et al. Antimicrobial activity of Croton cajucara benth linalool-rich essential oil on artificial biofilms and planktonic microorganisms. Oral Microbiol Immunol 2005;20:101–5.
18. Antonious GF, Kochhar TS. Zingiberene and curcumene in wild tomato. J Environ Sci Health Part B 2003;38:489-500.
19. Filly A, Fabiano Tixier AS, Louis C, Fernandez X, Chemat F. Water as a green solvent combined with different techniques for extraction of essential oil from lavender flowers. C R Chimie 2016;19:707-17.
20. Ozek G, Ozek T, Iscan G, Baser KHC, Hamzaoglu E, Duran A. Comparison of hydrodistillation and microdistillation methods for the analysis of fruit volatiles of Prangos pabularia lindl., and evaluation of its antimicrobial activity. South Afr J Bot 2007;73:563–9.
21. Amelia B, Saepudin E, Cahyana AH, Rahayu DU, Sulistyoningrum AS, Haib J. GC-MS analysis of clove (Syzygium aromaticum) bud essential oil from Java and Manado. AIP Conf Proc 2017;1862:030082.
22. Shettima AY, Karumi Y, Sodipo OA, Usman H, Tijjani MA. Gas chromatography-mass spectrometry (GC-MS) analysis of bioactive components of ethyl acetate root extract of Guiera senegalensis J F Gmel. J Appl Pharm Sci 2013;3:146-50.
23. Delazar A, Reid RG, Sarker SD. GC-MS analysis of essential oil of the oleoresin from Pistacia atlantica var mutica. Chem Nat Compounds 2004;40:24–7.
24. Brophy JJ, Rahmani M, Toia RF, Croft KD, Lassak EV. The volatile oils of Euodia hortensis forma hortensis. Flav Frag J 1985;1:17-20.
25. Handayani PA, Nurcahyanti H. Ekstraksi minyak atsiri daun zodia (Evodia suaveolens) dengan metode maserasi dan distilasi air. Jurnal Bahan Alam Terbarukan 2015;4:1-7.
Published
15-07-2020
How to Cite
SIDHARTA, B. R., and P. K. ATMODJO. “GAS CHROMATOGRAPHY–MASS SPECTROMETRY ANALYSIS OF BIOACTIVE COMPOUNDS IN ESSENTIAL OILS OF LEAF OF EUODIA SUAVEOLENS SCHEFF”. International Journal of Current Pharmaceutical Research, vol. 12, no. 4, July 2020, pp. 139-42, doi:10.22159/ijcpr.2020v12i4.39102.
Issue
Section
Original Article(s)
