PHOENIX DACTYLIFERA FRUIT EXTRACT AMELIORATES ALTERED BIOCHEMICAL PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETES MELLITUS ALBINO RATS
DOI:
https://doi.org/10.22159/ijcpr.2020v12i6.40275Keywords:
Phoenix dactylifera, Diabetes mellitus, Hypoglycemic, Hypolipidemic, Fruit extract, NutraceuticalAbstract
Objective: Consistent projections have indicated a steady increase in the global burden of diabetes mellitus. Given the increased cost in conventional management of the condition in addition to reported side effects and cost of orthodox management, attention is shifting to the use of alternative methods such as plants materials with dual benefits as food and medicine. Thus, this study was designed to investigate the effect of date palm fruit on some biochemical parameters in streptozotocin-induced wistar rats.
Methods: Animals were divided into 5 groups of 6 rats each (I as normal control, II as diabetic control and 3-5 as extract-treated groups) maintained for 14 d. At the end of the treatment, the animals were fasted overnight, then sacrificed and blood samples collected for analysis of biochemical parameters (including blood glucose, blood lipids and enzymes).
Results: The results show that treatment of diabetic animals with extract of date palm fruit show a significant (P<0.05) reduction in glucose levels in groups II–V compared to group I. Similar positive effects were observed in the levels of lipids and enzymes in treated groups compared to diabetic control group II. A GC-MS analysis of the fraction of the fruit extract revealed some bioactive compounds that may be responsible for the effects exhibited in the study.
Conclusion: These findings which demonstrate the ameliorative effect on hyperglycemia and hyperlipidemia, further support the use of date palm fruit as a nutraceutical agent.
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References
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26. Fernandes AAH, Barbosa EL, Novelli AF. Junior and cristiano machado galhardi effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats. Brazilian Arch Biol Technol 2009;52:51-9.
27. Mathur S, Mehta DK, Kapoor S, Yadav S. Liver function in type-2 diabetes mellitus patients. Int J Sci Stud 2016;3:43–7.
28. Michael HN, Salib JY, Eskander EF. Bioactivity of diosmetin glycosides isolated from the epicarp of date fruits, phoenix dactylifera, on the biochemical profile of alloxan diabetic male rats. Phytother Res 2012;14:1-6.
29. Hasan M, Mohieldein A. In vivo evaluation of anti-diabetic, hypolipidemic, antioxidative activities of saudi date seed extract on streptozotocin-induced diabetic rats. J Clin Diagnostic Res 2016;10:6–12.
30. El-Mergawi RA, AlGeffari MA, Al-Humaid A. Sugar types, phenolic contents, and antioxidant activities for 17 Saudi Arabian date cultivars and their relations with glycemic indices. Int J Fruit Sci 2018 https://doi.org/10.1080/15538362.2018.1535356
31. Ayatollahi SA, Sharifi Rad M, Roointan A, Baghalpour N, Salehi B, Shinwari ZK, et al. Antidiabetic activity of date seed methanolic extracts in alloxan-induced diabetic rats. Pak Vet J 2019;39:583-7.
32. Gerber PA, Rutter GA. The role of oxidative stress and hypoxia in pancreatic beta-cell dysfunction in diabetes mellitus. Antioxidants Redox Signaling 2017;26:501-17.
33. Sudha P, Smita SZ, Shobha YB, Kumar AR. Potent ?-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complement Altern Med 2011;5:11-5.
34. Oyedemi SO, Adewusi EA, Aiyegoro OA, Akinpelu DA. Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin-induced diabetic wistar rats. Asian Pac J Trop Biomed 2011;1:353-8.
35. Ahmed S, Khan RA, Subia J. Antihyperlipidemic and hepatoprotective effects of native date fruit variety "Aseel" (Phoenix Dactylifera). Pak J Pharm Sci 2016;29:1945-50.
2. Piero MN, Nzaro GM, Njagi JM. Diabetes mellitus–a devastating metabolic disorder. Asian J Biomed Pharm Sci 2014;4:1-7.
3. American Diabetes Association (ADA). Classification and Diagnosis of Diabetes. Diabetes Care 2015;38:S8–S16.
4. World Health Organization (WHO). Classification of Diabetes Mellitus. Geneva; 2019. p. 2-29.
5. International Diabetes Federation (IDF). Diabetes Atlas. 8th Edition. Brussels; 2017.
6. Uloko AE, Musa BM, Ramalan MA, Gezewa ID, Puepet FH, Uloko AT, et al. Prevalence and risk factors for diabetes mellitus in nigeria: a systematic review and meta-analysis. Diabetes Ther 2018;9:1307?16.
7. Piero NM, Njagi MJ, Kibiti MC, Ngeranwa JN, Njagi NM, Eliud NM. Wilson GK. Herbal management of diabetes mellitus: a rapidly expanding research avenue. Int J Curr Pharm Res 2012;4:1-4.
8. Rehan T, Khalid IK, Rizwan AM, Zafeer NW. Natural remedies for diabetes mellitus. Int Curr Pharm J 2016;5:97-102.
9. Alkaabi JM, Al-Dabbagh B, Ahmad S, Saadi HF, Gariballa S, Al Ghazali M. Glycemic indices of five varieties of dates in healthy and diabetic subjects. Nutr J 2011;10:59.
10. Eoin LN. Systematics: blind dating. Nat Plants 2016;2:16069.
11. Mohamed RMA, Fageer ASM, Eltayeb MM, Ahmed IAM. Chemical composition, antioxidant capacity, and mineral extractability of sudanese date palm (Phoenix dactylifera L.) fruits. Food Sci Nutr 2014;2:478–89.
12. Oni SO, Abiola MA, Ladokun OA, Ighodaro OM, Oyedele OM. Nutritional and phytochemical profile of niger cultivated date palm (Phoenix Dactilyfera L). J Food Nutr Sci 2015;3:114-8.
13. Yahaya SA, Ahmed FO, Gana SA, Daudu OA, Yusuf ABM, Osaro-odin PE, et al. Nutritional properties of some selected date palm (Phoenix dactylifera L) landraces in nigeria. World J Food Sci Technol 2020;4:1-7.
14. Yahaya SA, Omokhudu CA, Abdulahi MA, Sanusi MK. Phytochemical screening and mineral evaluation of fresh date fruits (Phoenix dactylifera L.) in wet season of Nigeria. J Agric Crop Res 2015;3:47-52.
15. Hafez El-Far JA, Shaheen HM, Abd El-Daim M, Al Jaouni SK, Mousa JSA. Date palm (Phoenix dactylifera): protection and remedy food. Curr Trends Nutr 2016:1:2.
16. Saryono S. Date seeds drinking as antidiabetic: a systematic review IOP conf. series: Earth and Environmental Science; 2019. p. 255.
17. Abiola MA, Oni SO, Olusola AL, Osasenaga MI, Omotayo MO. Nutritional and phytochemical profile of niger cultivated date palm (Phoenix Dactilyfera L). J Food Nutr Sci 2015;3:114-8.
18. Akbarzadeh AD, Norouzian MR, Mehrabi SH, Jamshidi A, Farhangi A, Allah Verdi SMA, et al. Induction of diabetes by streptozotocin in rats. Indian J Clin Biochem 2007;22:60-4.
19. Furman BL. Streptozotocin-induced diabetic models in mice and rats. Curr Protoc Pharmacol 2015;70:47-54.
20. Deeds MC, Anderson JM, Armstrong AS, Gastineau DA, Hiddinga HJ, Jahangir A. Single-dose streptozotocin-induced diabetes: considerations or study design in islet transplantation models. NLLab Anim 2011;45:131–40.
21. Chaimum Aom A, Chomko S, Talubmook C. Toxicology and oral glucose tolerance test (OGTT) of thai medicinal plant used for diabetes controls, phyllanthus aci-dus L. (Euphorbiaceae). Pharmacogn J 2017;9:58-61.
22. Bexfield AB, Morgan AE, Wagstaff CJ, Newton RP, Ratcliffe NA, Nigam Y. Amino acid derivatives from Lucilia sericata excretions?secretions may contribute to the beneficial effects of maggot therapy via increased angiogenesis. Br J Dermatol 2010;162:554–62.
23. AlGeffari MA, Almogbel ES, Alhomaidan HT, El-Mergawi R, Barrimaha IA. Glycaemic indices, glycaemic load and glycaemic response for seventeen varieties of dates grown in Saudi Arabia. Ann Saudi Med 2016;36:397-403.
24. El-Mousalamy AMD, Hussein AAM, Mahmoud SA, Abdelaziz A, Shaker G Aqueous. Methanolic extracts of palm date seeds and fruits (Phoenix dactylifera) protects against diabetic nephropathy in type ii diabetic rats. Biochem Physiol 2016;5:205.
25. Shibabaw T, Dessie G, Molla MD, Zerihun MF, Ayelign B. Assessment of liver marker enzymes and its association with type 2 diabetes mellitus in Northwest Ethiopia. BMC Res Notes 2019;12:707.
26. Fernandes AAH, Barbosa EL, Novelli AF. Junior and cristiano machado galhardi effect of naringerin on biochemical parameters in the streptozotocin-induced diabetic rats. Brazilian Arch Biol Technol 2009;52:51-9.
27. Mathur S, Mehta DK, Kapoor S, Yadav S. Liver function in type-2 diabetes mellitus patients. Int J Sci Stud 2016;3:43–7.
28. Michael HN, Salib JY, Eskander EF. Bioactivity of diosmetin glycosides isolated from the epicarp of date fruits, phoenix dactylifera, on the biochemical profile of alloxan diabetic male rats. Phytother Res 2012;14:1-6.
29. Hasan M, Mohieldein A. In vivo evaluation of anti-diabetic, hypolipidemic, antioxidative activities of saudi date seed extract on streptozotocin-induced diabetic rats. J Clin Diagnostic Res 2016;10:6–12.
30. El-Mergawi RA, AlGeffari MA, Al-Humaid A. Sugar types, phenolic contents, and antioxidant activities for 17 Saudi Arabian date cultivars and their relations with glycemic indices. Int J Fruit Sci 2018 https://doi.org/10.1080/15538362.2018.1535356
31. Ayatollahi SA, Sharifi Rad M, Roointan A, Baghalpour N, Salehi B, Shinwari ZK, et al. Antidiabetic activity of date seed methanolic extracts in alloxan-induced diabetic rats. Pak Vet J 2019;39:583-7.
32. Gerber PA, Rutter GA. The role of oxidative stress and hypoxia in pancreatic beta-cell dysfunction in diabetes mellitus. Antioxidants Redox Signaling 2017;26:501-17.
33. Sudha P, Smita SZ, Shobha YB, Kumar AR. Potent ?-amylase inhibitory activity of Indian Ayurvedic medicinal plants. BMC Complement Altern Med 2011;5:11-5.
34. Oyedemi SO, Adewusi EA, Aiyegoro OA, Akinpelu DA. Antidiabetic and haematological effect of aqueous extract of stem bark of Afzelia africana (Smith) on streptozotocin-induced diabetic wistar rats. Asian Pac J Trop Biomed 2011;1:353-8.
35. Ahmed S, Khan RA, Subia J. Antihyperlipidemic and hepatoprotective effects of native date fruit variety "Aseel" (Phoenix Dactylifera). Pak J Pharm Sci 2016;29:1945-50.
Published
15-11-2020
How to Cite
A. O., O., A. S. F., I. B., and E. U. E. “PHOENIX DACTYLIFERA FRUIT EXTRACT AMELIORATES ALTERED BIOCHEMICAL PARAMETERS IN STREPTOZOTOCIN-INDUCED DIABETES MELLITUS ALBINO RATS”. International Journal of Current Pharmaceutical Research, vol. 12, no. 6, Nov. 2020, pp. 27-31, doi:10.22159/ijcpr.2020v12i6.40275.
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