PHYTOCHEMICAL ANALYSIS AND IN VITRO DPPH ASSAY OF ETHANOLIC EXTRACT OF MUCUNA PRURIENS SEED
DOI:
https://doi.org/10.22159/ijcpr.2020v12i6.40300Keywords:
Mucuna pruriens, Reactive oxygen species, In vitro antioxidant activity, DPPH assay, Natural antioxidants, FlavonoidsAbstract
Objective: To detect the bioactive phytoconstituents of Mucuna pruriens seed extract and to estimate invitro antioxidant activity.
Methods: Preliminary phytochemical analysis were done to determine the active phytochemical constituents and DPPH (2, 2-diphenyl-1-picrylhydrazyl) assay method was used for the estimation of In vitro antioxidant activity.
Results: Preliminary phytochemical analysis of Mucuna pruriens seed extract showed the presence of chief bioactive components like phenols, flavonoids, tannins, alkaloids, triterpenes and phytosterols. In antioxidant assay, Mucuna pruriens seed extract exhibited a maximum DPPH radical scavenging activity of 72.95% against the standard control with a maximum activity of 93.24% for the same concentration (0.2 mg/ml). This is a clear indication of strong antioxidant property of the drug.
Conclusion: Mucuna Pruriens seeds are abundant source of natural antioxidants, and important bioactive phytoconstituents.
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References
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2. Nita M, Grzybowski A. The role of the reactive oxygen species and oxidative stress in the pathomechanism of the age-related ocular diseases and other pathologies of the anterior and posterior eye segments in adults. Oxid Med Cell Longev 2016;2016:3164734. DOI:10.1155/2016/3164734
3. Grune T, Shringarpure R, Sitte N, Davies K. Age-related changes in protein oxidation and proteolysis in mammalian cells. J Gerontol A Biol Sci Med Sci 2001;56:B459-67.
4. Noguchi N, Niki E. Phenolic antioxidants: a rationale for design and evaluation of novel antioxidant drug for atherosclerosis. Free Radical Biol Med 2000;28:1538-46.
5. Dias V, Junn E, Mouradian MM. The role of oxidative stress in Parkinson's disease. J Parkinsons Dis 2013;3:461-91.
6. Harris, Isaac S, Gina M DeNicola. The complex interplay between antioxidants and ROS in cancer. Trends in Cell Biology; 2020.
7. Devaraj, Sridevi. Supplementation with pine bark extract rich in polyphenols increases plasma antioxidant capacity and alters the plasma lipoprotein profile. Lipids 2002;37:931-4.
8. Lohiya NK, K Balasubramanian, AS Ansari. Indian folklore medicine in managing men's health and wellness. Andrologia 2016;48:894-907.
9. Rajesh R. The effect of mucuna pruriens seed extract on pancreas and liver of diabetic wistar rats. Int J Curr Res Rev 2016;8:61.
10. Deokar, Gitanjali, Harshada Kakulte, Sanjay Kshirsagar. Phytochemistry and pharmacological activity of Mucuna pruriens: a review. Pharm Biol Evaluations 2016;3:50-9.
11. Evans, William Charles. Trease and evans' pharmacognosy E-book. Elsevier Health Sciences; 2009.
12. Liyana Pathirana, Chandrika, Fereidoon Shahidi. Optimization of extraction of phenolic compounds from wheat using response surface methodology. Food Chem 2005;93:47-56.
13. Swamy, Gangadhara, Rajendra Holla, Suresh R Rao. A preliminary investigation for standardization of hydroalcoholic extract of mucuna pruriens (Kapikaccu): an important drug used to treat parkinson’s disease in ayurveda. J Health Allied Sci NU 2019;9:85-90.
14. Hadimani, Gavishiddappa A. Evaluation of acute oral toxicity and phytoconstituents of methanolic extract of Mucuna pruriens. J Pharma Sci Res 2015;7:33-6.
15. Dhami N, Mishra AD. Phytochemical variation: how to resolve the quality controversies of herbal medicinal products? J Herbal Med 2015;5:118–27.
16. Pham Huy LA, He H, Pham Huy C. Free radicals, antioxidants in disease and health. Int J Biomed Sci 2008;4:89-96.
17. Sharma, Om P, Tej K Bhat. DPPH antioxidant assay revisited. Food Chem 2009;113:1202-5.
18. Kennedy JF, Thorley M. Pharmacognosy, phytochemistry, medicinal plants. 2nd ed. Carbohydrates and Polymers; 2000;42:428–9.
19. Kumari M, Jain S. Tannins: an antinutrient with positive effect to manage diabetes. Res J Recent Sci 2012;1:70–3.
20. Rajesh R, Kotasthane DS, KM, Singh A, VS, Rajasekar SS. Histopathological and Histomorphometric analysis of Pancreas and liver of diabetic rats treated with mucuna pruriens seed extract. Annals Pathol Laboratory Med 2017;4:A546–52.
21. Uttara, Bayani. Oxidative stress and neurodegenerative diseases: a review of upstream and downstream antioxidant therapeutic options. Curr Neuropharmacol 2009;7:65-74.
22. Jang, Kyung Jun. Anti?inflammatory potential of total saponins derived from the roots of panax ginseng in lipopolysaccharide?activated RAW 264.7 macrophages. Exp Ther Med 2016;11:1109-15.
23. Rajesh R, Kotasthane DS, KM, Singh A, VS, Rajasekar SS. Histopathological and histomorphometric analysis of pancreas and liver of diabetic rats treated with mucuna pruriens seed extract. Ann Pathol Lab Med 2017;4:A546–52.
24. Wu Yan-Qin. Long chain omega-3 polyunsaturated fatty acid supplementation alleviates doxorubicin-induced depressive-like behaviors and neurotoxicity in rats: involvement of oxidative stress and neuroinflammation. Nutrients 2016;8:243.
25. Bhandari, Jaya. Study of phytochemical, anti-microbial, anti-oxidant, and anti-cancer properties of Allium wallichii. BMC Complementary Alternative Med 2017;17:102.
26. Amico, Vincenzo. Antiproliferative terpenoids from almond hulls (Prunus dulcis): identification and structure?activity relationships. J Agric Food Chem 2006;54:810-4.
27. Minari JB, GO Ogar, AJ Bello. Antiproliferative potential of aqueous leaf extract of mucuna pruriens on DMBA-induced breast cancer in female albino rats. Egyptian J Med Human Genetics 2016;17:331-43.
Published
15-11-2020
How to Cite
V., S., V. B., S. S. RAJASEKAR, and R. R. “PHYTOCHEMICAL ANALYSIS AND IN VITRO DPPH ASSAY OF ETHANOLIC EXTRACT OF MUCUNA PRURIENS SEED”. International Journal of Current Pharmaceutical Research, vol. 12, no. 6, Nov. 2020, pp. 93-96, doi:10.22159/ijcpr.2020v12i6.40300.
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