ANTIBACTERIAL POTENTIAL OF ESSENTIAL OILS DERIVED FROM NATURAL, CALLUS AND IN-VITRO PROPAGATED SOURCES OF MELALEUCA ALTERNIFOLIA AGAINST COMMON BACTERIAL PATHOGENS
DOI:
https://doi.org/10.22159/ijcpr.2021v13i3.42081Keywords:
M alternifolia, EOIPL, Minimum inhibitory concentration, Terpinen-4-ol, Molecular dockingAbstract
Objective: Melaleuca alternifolia (M. alternifolia) and its essential oil (EO) fractions have been used widely and traditionally in the treatment of various infectious diseases and hence its antibacterial potential is investigated in the present study.
Methods: The antibacterial activity was studied through the agar disc diffusion method and broth dilution method, DNA fragmentation studies and confocal microscopy morphological studies were done. In-silico molecular interaction was studied against microbial target using docking software.
Results: The inhibitory concentration of the EOs was recorded at 75% dilution with larger inhibition zones. The DNA fragmentation analyzed in the essential oil derived from in-vitro propagated leaves (EOIPL) of M. alternifolia treated bacterial cultures was compared with negative and positive controls. In Minimum Inhibitory Concentration (MIC) of EOIPL treated Staphylococcus aureus (S. aureus) showed time-dependent growth inhibition. The DNA content in the EOIPL treated bacterial cultures was comparatively less than in control cultures. The cell morphology changes of S. aureus cells were studied through confocal laser scanning microscopic analysis which showed a significant decrease in viable bacterial cells. The active component, terpinen-4-ol docked to autolysin receptor revealed stable interaction with the microbial target.
Conclusion: Thus EOIPL was explored to possess bactericidal activity against common infectious bacteria and could in incorporated in therapeutic natural antibiotic formulations as with future studies.
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2. Mamta, Jakhar KK. Studies on in-vitro antibacterial activity of Tinospora cordifolia stem extract on Escherichia coli. Vet Res Int 2016;4:74-7.
3. Snoussi M, Hajlaoui H, Noumi E, Zanetti S, Bakhrouf A. Phenotypic and genetic diversity of Vibrio alginolyticus strains recovered from juveniles and older Sparus aurata reared in a Tunisian marine farm. Ann Microbiol 2008;58:141-6.
4. Carson CF, Hammer KA, Riley TV. Melaleuca alternifolia (Tea Tree) Oil: a Review of antimicrobial and other medicinal properties. Clin Microbiol Rev 2006;19:50-62.
5. Melvin P. Weinstein, MD. Performance standards for antimicrobial susceptibility testing. NCCLS Document 2002(Suppl 12);100-12.
6. Duncan BD. Multiple range tests for correlated and heteroscedastic means. Biom 1957;13:164-7.
7. Cerca N, Gomes F, Pereira S, Teixeira P, Oliveira R. Confocal laser scanning microscopy analysis of Streptococcus epidermidis biofilms exposed to farnesol, vancomycin and rifampicin. BMC Res Notes 2012;5:244.
8. Berman HM, Westbrook J, Feng Z, Gilliland G, Bhat TN, Weissig H, et al. The protein data bank. Nucleic Acids Res 2000;28:235-42.
9. Daisy P, Eliza J, Ignacimuthu S. Influence of Costus speciosus (Koen.) Sm. rhizome extracts on biochemical parameters in streptozotocin induced diabetic rats. J Health Sci 2008;54:675-81.
10. Lee C, Chen L, Chen L, Chang T, Huang C, Huang M, et al. Correlations of the components of tea tree oil with its antibacterial effects and skin irritation. J Food Drug Anal 2013;21:169-76.
11. Calcabrini A, Stringaro A, Toccacieli L, Meschini S, Marra M, Colone M, et al. Terpinen-4-ol, the main component of Melaleuca alternifolia (tea tree) oil inhibits the in-vitro growth of human melanoma cells. J Invest Dermatol 2004;122:349-60.
12. Jakub K, Sigrun E, Kinga W. Effects of tea tree (Melaleuca alternifolia) oil on Staphylococcus aureus in biofilms and stationary growth phase. Int J Antimicrob Agents 2009;33:343-7.
13. El-Tablawy SY, Araby E. Impact of marjoram (Origanum marjorana L.) essential oil on some virulence factors and DNA integrity of multidrug resistant Klebsiella pneumoniae. IOSR J Pharm Biol Sci 2017;12:63-71.
14. Corliss AO, Sean JP, Philip GC. Steven CR. Potential of plant essential oils and their components in animal agriculture-in-vitro studies on antibacterial mode of action. Front Vet Sci 2015;2:197-9.
15. Reck M, Rutz K, Kunze B, Tomasch J, Surapaneni S, Schulz S, Wagner Dobler I. The biofilm inhibitor carolacton disturbs membrane integrity and cell division of streptococcus mutans through the serine/threonine protein kinase PknB. J Bacteriol 2011;193:5692-706.
16. Ya C, Gaffney SH, Lilley TH, Haslam E. Carbohydrate-polyphenol complexation. In: Hemingway RW, Karchesy JJ. eds. Chemi and signify of conden. Tan. New York, NY: Plenum Press; 1988. p. 553.
17. Cowan MM. Plant products as antimicrobial agents. Clin Microbiol Rev 1999;12:564-82.
18. Daisy P, Suveena S, Lilly VJ. Molecular docking of medicinal compound Lupeol with autolysin and potential drug target of UTI. J Chem Pharm Res 2011;3:557-62.
19. Daisy P, Vijayalakshmi P, Selvaraj C, Singh SK, Saipriya K. Targeting multidrug resistant mycobacterium tuberculosis HtrA2 with Identical chemical entities of fluoroquinolones. Indian J Pharm Sci 2012;74:217-22.
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