APOPTOSIS INDUCING EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING MAGNOLIA CHAMPACA LEAF EXTRACT ON MCF-7 CELL LINE
DOI:
https://doi.org/10.22159/ijcpr.2021v13i3.42083Keywords:
Magnolia champaca, Green-synthesis, Anticancer, Apoptosis, Caspase-3Abstract
Objective: To synthesize silver nanoparticle (AgNP) using the ethanolic leaf extract of Magnolia champaca (MC-AgNP) and to evaluate its cytotoxicity and apoptotic activity in MCF-7 cell line, and to analyse active Caspase-3 expression in inducing apoptosis.
Methods: AgNP was green synthesized using ethanolic leaf extract of MC. Shape and size were analysed by SEM and particle size analyser. Cytotoxicity was tested to find IC50 value. Apoptosis was explored by mitochondrial membrane potential (ΔΨm) assay and active Caspase-3 expression study using flow cytometry.
Results: AgNP synthesized was observed to be in spherical shape through SEM micrograph with 164.6 nm in size. IC50 value of 15.90 µg/ml was obtained after 24 h of drug treatment. Changes in ΔΨm and positive active Caspase-3 protein expression were observed.
Conclusion: The present results suggest that MC-AgNP has potential anticancer property and also induced cell death via apoptosis through caspase dependent pathway in MCF-7 cells.
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References
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2. Com?a S, Cimpean AM, Raica M. The story of MCF-7 breast cancer cell line: 40 y of experience in research. Anticancer Res 2015;35:3147-54.
3. Jamalzadeh L, Ghafoori H, Aghamaali M, Sariri R. Induction of apoptosis in human breast cancer MCF-7 cells by a semisynthetic derivative of artemisinin: a caspase-related mechanism. Iran J Biotechnol 2017;15:157-65.
4. Newman DJ, Cragg GM. Natural products as sources of new drugs from 1981 to 2014. J Nat Prod 2016;79:629-61.
5. Ren Y, Blanco E, Fuchs JR, Soejarto DD, Burdette JE, Swanson S, et al. Potential anticancer agents characterized from selected tropical plants. J Nat Prod 2019;82:657-79.
6. Rout PK, Naik S, Rao YR. Liquid CO2 extraction of flowers and fractionation of floral concrete of Michelia champaca linn. J Supercrit Fluids 2011;56:249-52.
7. Fernando MTR, Jayasuriya G, Walck JL, Wijetunga ASTB. Identifying dormancy class and storage behaviour of champak (Magnolia champaca) seeds, an important tropical timber tree. J Nat Sci Found 2013;41:141-6.
8. Sinha R, Varma R. Michelia champaca L. (Swarna Champa): a review. Int J Enhanc Res Sci Technol Eng 2016;5:78-82.
9. Khan MR, Kihara M, Omoloso A. Antimicrobial activity of Michelia champaca. Fitoterapia 2002;73:744-8.
10. Hasan SMR, Hossain MM, Akter R, Jamila M, Mazumder EH, Rahman S. DPPH free radical scavenging activity of some Bangladeshi medicinal plants. J Med Plant Res 2009;3:875-9.
11. Jarald EE, Joshi SB, Jain DC. Antidiabetic activity of flower buds of Michelia champaca linn. Indian J Pharmacol 2008;40:256-60.
12. Hoffmann JJ, Torrance SJ, Wiedhopf RM, Cole JR. Cytotoxic agents from Michelia champaca and Talauma ovata: parthenolide and costunolide. J Pharma Sci 1977;66:883-4.
13. Vimala R, Nagarajan S, Alam M, Susan T, Joy S. Antiinflammatory and antipyretic activity of Michelia champaca linn. (White variety), Ixora brachiata Roxb. and Rhynchosia cana (Wild.) D. C. flower extract. Indian J Exp Biol 1997;35:1310-4.
14. Takahashi M, Fuchino H, Satake M, Agatsuma Y, Sekita S. In vitro screening of leishmanicidal activity in Myanmar timber extracts. Biol Pharm Bull 2004;27:921-5.
15. Ahmed H, Saxena V, Mishra A, Gupta R. Diuretic activity of aqueous extracts of Michelia champaca L. leaves and stem bark in rats. Pharmacologyonline 2011;2:568-74.
16. Armendariz V, Herrera I, Peralta Videa JR, Jose Yacaman M, Troiani H, Santiago P, et al. Size controlled gold nanoparticle formation by Avena sativa biomass: use of plants in nanobiotechnology. J Nanoparticles Res 2004;6:377-82.
17. Bhumkar DR, Joshi HM, Sastry M, Pokharkar VB. Chitosan reduced gold nanoparticles as novel carriers for transmucosal delivery of insulin. Pharm Res 2007;24:1415-26.
18. Parimi U, Amperayani KR. Green synthesis of plant mediated silver nano particles and evaluation of their antimicrobial activities. Am J PharmTech Res 2014;4:673-9.
19. Ajiboye BO, Ibukun EO, Edobor G, Ojo AO, Onikanni SA. Qualitative and quantitative analysis of phytochemicals in Senecio biafrae leaf. Int J Pharm Sci Invent 2013;1:428-32.
20. Visweswari G, Christopher R, Rajendra W. Phytochemical screening of active secondary metabolites present in Withania somnifera root: role in traditional medicine. Int J Pharm Sci Res 2013;4:2770-6.
21. Ruwali P, Ambwani TK, Gautam P, Thapliyal A. Qualitative and quantitative phytochemical analysis of Artemisia indica willd. J Chem Pharm Res 2015;7:942-9.
22. Vijayanand S, Thomas A. Screening of Michelia champaca and Muntingia calabura extracts for potential bioactives. Int J Pharm Sci Res 2016;7:266-73.
23. Gul R, Jan SU, Faridullah S, Sherani S, Jahan N. Preliminary phytochemical screening, quantitative analysis of alkaloids and antioxidant activity of crude plant extracts from Ephedra intermedia indigenous to Balochistan. Sci World J 2017:1-7. https://doi.org/10.1155/2017/5873648
24. Bahuguna A, Khan I, Bajpai V, Kang S. MTT assay to evaluate the cytotoxic potential of a drug. Bangladesh J Pharmacol 2017;12:115-8.
25. Tran QA, Nguyen VQ, Le A. Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Adv Nat Sci Nanosci 2013;4:1-20.
26. Mosmann T. Rapid colorimetric assay for cellular growth and survival: application to proliferation and cytotoxicity assays. J Immunol Methods 1983;65:55-63.
27. Riccardi C, Nicoletti I. Analysis of apoptosis by propidium iodide staining and flow cytometry. Nat Protoc 2006;1:1458-61.
28. Green DR, Reed JC. Mitochondria and apoptosis. Science 1998;281:1309-12.
29. Salvioli S, Ardizzoni A, Franceschi C, Cossarizza A. JC-1, but not DiOC6 or rhodamine 123, is a reliable fluorescent probe to assess ?? changes in intact cells: implications for studies on mitochondrial functionality during apoptosis. FEBS Lett 1997;411:77-82.
30. Salvioli S, Dobrucki J, Moretti L, Troiano L, Fernandez MG, Pinti M, et al. Mitochondrial heterogeneity during staurosporine-induced apoptosis in HL60 cells: analysis at the single cell and single organelle level. Cytometry 2000;40:189–97.
31. Luo J, Hu Y, Kong W, Yang M. Evaluation and structure-activity relationship analysis of a new series of arylnaphthalene lignans as potential anti-tumor agents. PLoS One 2014;9:1-10.
Published
15-05-2021
How to Cite
ANTONY, A., N. SHRIVASTAV, S. DUBEY, and A. ACHARYA. “APOPTOSIS INDUCING EFFECT OF SILVER NANOPARTICLES SYNTHESIZED USING MAGNOLIA CHAMPACA LEAF EXTRACT ON MCF-7 CELL LINE”. International Journal of Current Pharmaceutical Research, vol. 13, no. 3, May 2021, pp. 14-18, doi:10.22159/ijcpr.2021v13i3.42083.
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