IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF CENTELLA ASIATICA LINN. IN INDIAN ADULT EARTHWORMS

SWAGATA DATTA; GEETANJALI NINGTHOUJAM; CHRISTINA ZOSANGPUII; PAONAM SHYAMASAKHI; NAMEIRAKPAM MEENA

doi:10.22159/ijcpr.2021v13i5.1884

Authors

SWAGATA DATTA Regional Institute of Medical Sciences, Department of Pharmacology, Imphal, Manipur, India, Pin 795004
GEETANJALI NINGTHOUJAM Regional Institute of Medical Sciences, Department of Pharmacology, Imphal, Manipur, India, Pin 795004
CHRISTINA ZOSANGPUII Regional Institute of Medical Sciences, Department of Pharmacology, Imphal, Manipur, India, Pin 795004
PAONAM SHYAMASAKHI Regional Institute of Medical Sciences, Department of Pharmacology, Imphal, Manipur, India, Pin 795004
NAMEIRAKPAM MEENA Regional Institute of Medical Sciences, Department of Pharmacology, Imphal, Manipur, India, Pin 795004

DOI:

https://doi.org/10.22159/ijcpr.2021v13i5.1884

Keywords:

Helminthiasis, Centella asiatica Linn., Pheretima posthuma, Albendazole

Abstract

Objective: Helminthiasis is one of the most prevalent parasitic infestations worldwide posing a major threat to public health. The control of these nematodes has relied largely on the use of a limited number of anthelmintics. However, emerging resistance and side effects to the currently available anthelmintic drugs is a major concern and discovery of newer anthelmintics with a novel mode of action is the need of the hour. The present study is aimed to evaluate the anthelmintic activity of ethanolic extract of Centella asiatica Linn. (EECA) on Indian earthworms (Pheretima posthuma).

Methods: The earthworms were divided into 4 groups with 6 worms in each group. The anthelmintic activity of EECA at two different concentrations (25 mg/ml and 50 mg/ml) was evaluated by assessing the time of paralysis and time of death of the worms. Albendazole was used as standard and 2% gum acacia as control.

Results: Albendazole at 25 mg/ml showed the highest anthelmintic activity and had significant difference (p<0.001) with EECA at both 25 mg/ml and 50 mg/ml.

Conclusion: Both doses of the test drug showed anthelmintic activity but the extract at either dose was found to be less effective than the standard drug. Further studies with higher doses of the extract should be done to evaluate the anthelmintic activity in a dose-dependent manner.

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