ISOLATION AND SCREENING OF MARINE BACTERIAL ISOLATES AGAINST CLINICAL PATHOGENS

Authors

  • D. SANGEETHA Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India
  • J. THARANI Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India
  • R. ISHWARYA Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India
  • P. RAMYA Department of Microbiology, Faculty of Science, Annamalai University, Annamalai Nagar 608002, Tamil Nadu, India

DOI:

https://doi.org/10.22159/ijcpr.2022v14i3.1987

Keywords:

Marine bacteria, Secondary metabolites, Antibiotic proteins, Clinical pathogens

Abstract

Objective: The marine organism produce a large array of metabolites with relevants in drug discovery, these metabolites have biological activity such as antibacterial, antioxidant and antitumor. The present study was undertaken to isolate marine bacteria from the marine soil sample and were tested against clinical pathogens.

Methods: The soil sample were collected in a sterile plastic sachet. The isolates were enumerated using the spread plate technique in Zobell marine agar. The ten isolated marine bacteria were tested against six clinical pathogens by the point inoculation method and agar well diffusion method.

Results: Among ten bacterial isolates M5 exhibited a maximum antibacterial activity and it was identified as Pseudomonas japonica by 16S rRNA sequencing.

Conclusion: It can be concluded that isolation of Marine bacterial samples can offer a numbers of microbial strains for sources of new biomolecules from Marine sources.

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References

Wilson CA, Harold Stevenson LH. The dynamics of the bacterial population associated with a salt marsh. J Exp Mar Biol Ecol. 1980;48(2):123-38. doi: 10.1016/0022-0981(80)90012-X.

Nair S, Simidu U. Distribution and significance of heterotrophic marine bacteria with antibacterial activity. Appl Environ Microbiol. 1987;53(12):2957-62. doi: 10.1128/aem.53.12.2957-2962.1987, PMID 3435149.

Austin B. Quantification of marine microbial populations. In: Austin B. editor. Marine microbiology. Cambridge University Press; 1992. p. 31-42.

Rosenfeld WD, Zobell CE. Antibiotic production by marine microorganisms. J Bacteriol. 1947;54(3):393-8. doi: 10.1128/jb.54.3.393-398.1947, PMID 16561373.

Grein A, Meyers SP. Growth characteristics and antibiotic production of actinomycetes isolated from littoral sediments and materials suspended in seawater. J Bacteriol. 1958;76(5):457-63. doi: 10.1128/jb.76.5.457-463.1958, PMID 13598702.

Wratten SJ, Wolfe MS, Andersen RJ, FaulkneR DJ. Antibiotic metabolites from a marine pseudomonad. Antimicrob Agents Chemother. 1977;11(3):411-4. doi: 10.1128/AAC.11.3.411, PMID 324390.

Tagg JR, Dajani AS, Wannamaker LW. Bacteriocins of gram-positive bacteria. Bacteriol Rev. 1976;40(3):722-56. doi: 10.1128/br.40.3.722-756.1976, PMID 791239.

Klaenhammer TR. Bacteriocins of lactic acid bacteria. Biochimie. 1988;70(3):337-49. doi: 10.1016/0300-9084(88)90206-4, PMID 3139051.

Chitnis V, Patil S, Kant R. Hospital effluent: a source of multiple drug-resistant bacteria. Curr Sci. 2000;79:83-4.

Breiman RF, Butler JC, Tenover FC, Elliott JA, Facklam RR. Emergence of drug-resistant pneumococcal infections in the United States. JAMA. 1994;271(23):1831-5, PMID 8196139.

Lechevalier HA, Corke CT. The replica plate method for screening antibiotic-producing organisms. Appl Microbiol. 1953;1(2):110-2. doi: 10.1128/am.1.2.110-112.1953, PMID 13031518.

Shewan JM, Hobbs G, Hodgkiss W. A determinative scheme for the identification of certain genera of gram-negative bacteria, with special reference to the pseudomonadaceae. J Appl Bacteriol. 1960;23(3):379-90. doi: 10.1111/j.1365-2672.1960.tb00211.x.

Amanda S, Motta F Cladera-Olivera, Brandelli A. Screening for antimicrobial activity among bacteria isolated from the Amazon basin. Braz J Microbiol. 2004;35:307-10.

Girmay Kalayu. Phosphate solubilizing microorganisms: promising approach as biofertilizers. Int J Agro. 2019. https://doi.org/10.1155/2019/4917256

Burkholder PR, Pfister RM, Leitz FH. Production of a pyrrole antibiotic by a marine bacterium. Appl Microbiol. 1966;14(4):649-53. doi: 10.1128/am.14.4.649-653.1966, PMID 4380876.

Published

15-05-2022

How to Cite

SANGEETHA, D., J. THARANI, R. ISHWARYA, and P. RAMYA. “ISOLATION AND SCREENING OF MARINE BACTERIAL ISOLATES AGAINST CLINICAL PATHOGENS”. International Journal of Current Pharmaceutical Research, vol. 14, no. 3, May 2022, pp. 53-57, doi:10.22159/ijcpr.2022v14i3.1987.

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