RP-HPLC-METHOD DEVELOPMENT AND VALIDATION FOR THE SIMULTANEOUS ESTIMATION OF DEXKETOPROFEN TROMETAMOL AND TRAMADOL HYDROCHLORIDE IN PHARMACEUTICAL DOSAGE FORM

P. SUSMITHA; CH. NEELIMA; M. HEMASRI; S. SRI LAKSHMI

doi:10.22159/ijcpr.2022v14i4.1983

Authors

P. SUSMITHA School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru, Technological University Kakinada, Kakinada 533003, Andhra Pradesh, India
CH. NEELIMA School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru, Technological University Kakinada, Kakinada 533003, Andhra Pradesh, India
M. HEMASRI School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru, Technological University Kakinada, Kakinada 533003, Andhra Pradesh, India
S. SRI LAKSHMI School of Pharmaceutical Sciences and Technologies, Jawaharlal Nehru, Technological University Kakinada, Kakinada 533003, Andhra Pradesh, India

DOI:

https://doi.org/10.22159/ijcpr.2022v14i4.1983

Keywords:

Reverse phase-high performance liquid chromatography, Validation, Dexketoprofen trometamol, Tramadol hydrochloride

Abstract

Objective: For the measurement and quantification of Dexketoprofen Trometamol and Tramadol Hydrochloride, a rapid, sensitive, and reliable RP-HPLC technique using the Waters HPLC System with PDA detection was designed and validated.

Methods: Chromatography was carried out on an Inertsil-ODS C18 (250 x 4.6 mm, 5) column with a flow rate of 1.0 ml/min and effluent of 240 nm using filtered and mixed Degassed Methanol: Buffer (75:25) v/v as a mobile phase.

Results: Dexketoprofen Trometamol and Tramadol hydrochloride has a retention time of 3.617 min and 5.013 min, respectively. % recovery for Dexketoprofen trometamol and tramadol hydrochloride was found to be 100.21% and 100.20%, respectively. LOD and LOQ values obtained for Dexketoprofen trometamol and tramadol hydrochloride are 0.074µg/ml, 0.225µg/ml and 0.175µg/ml, 0.531µg/ml respectively. Regression equation of Dexketoprofen trometamol is y = 7446.1273x-284.0702 and y = 39535.8782x-1580.4211 of Tramadol hydrochloride.

Conclusion: Dexketoprofen trometamol and tramadol hydrochloride has developed and validated in pharmaceutical dosage form using RP-HPLC method.

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