DETERMINATION OF S-METHYL L-CYSTEINE BY HIGH-PERFORMANCE LIQUID CHROMATOGRAPHIC METHOD
DOI:
https://doi.org/10.22159/ijcpr.2022v14i6.2048Keywords:
Amino acid determination, Analytical method validation, High performance liquid chromatography, S-Methyl L-Cysteine, 3-methylthioalanineAbstract
Objective: A simple, reproducible and sensitive high-performance liquid chromatography method has been developed for determination of S-Methyl L-Cysteine. S-Methyl L-Cysteine is widely observed and most common amino acid in plants, including many edible vegetables, which is responsible for reducing blood cholesterol level in the body.
Methods: S-Methyl L-Cysteine was chromatographed using Phosphate buffer of pH 6.5: Acetonitrile in the ratio of 97:3. The liquid chromatogram was equipped with a variable wavelength UV detector, an injector and a data processor. Inertsustain GL-Science Column C-18 (150 mm x 4.6 mm; 5μ) was used as a stationary phase.
Results: The retention time of S-Methyl L-Cysteine was observed as 2.261±0.0016 min. The linearity value for S-Methyl L-Cysteine was found to be 100-2000 µg/ml with Correlation of Determination (R2) value as 0.9992. LOD and LOQ values obtained are 29.51μg/ml and 89.74 μg/ml, respectively.
Conclusion: The method was developed and validated successfully as per ICH guidelines for analytical method validation.
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