ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITE FROM HABENARIA INTERMEDIA D. DON FOR SCREENING OF HEPATOPROTECITVE POTENTIAL AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY IN ALBINO RAT LIVER

Authors

  • Mahesh Anand Goudar DVS College of Arts and Science, Department of Chemistry, Kuvempu University, Shivamogga-577202, Karnataka, India
  • Jayadevappa H. Sahyadri Arts and Commerce College, Kuvempu University, Shivamogga-577202, Karnataka, India
  • Mahadevan K. M. Department of Postgraduate studies and Research in Pharmaceutical chemistry, Kuvempu University P. G. Center, Kadur-577550, Karnataka, India
  • Shastry R. A. Department of Pharmacognosy, Postgraduate studies and Research center, SETS College of Pharmacy, Dharwad-580002, Karnataka, India
  • Habbu P. V. Department of Pharmacognosy, Postgraduate studies and Research center, SETS College of Pharmacy, Dharwad-580002, Karnataka, India
  • Sayeswara H. A. Department of Zoology, Sahyadri Science College (Autonomous), Kuvempu University, Shivamogga-577202, Karnataka, India

Keywords:

Habenaria intermedia, Scopoletin, Hepatoprotecitve, CCl4, Silymarin

Abstract

The purpose of the study was to isolate the coumarin glycoside constituent from the ethyl acetate fraction of tubers of Habenaria intermedia D. Don by column chromatography using the gradient elution technique. The isolated coumarin glycoside was characterized by spectral studies and screened for hepatoprotecitve potential on CCl4 induced toxicity in rat liver. The coumarin glycoside (Scopoletin) protective activity was evaluated by the assay of liver function biochemical parameters such as SGOT, SGPT, Bilirubin, total protein and histopathological studies of the liver. Results showed that the toxic effect of CCl4 was controlled significantly by restoration of the levels of serum bilirubin, proteins and enzymes as compared to the normal and standard drug Silymarin treated groups. Histology of the liver sections of the rats treated with isolated coumarin glycoside showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further substantiated hepatoprotecitve activity. Therefore, outcome of the present study ascertains that the isolated coumarin glycoside possesses significant hepatoprotecitve activity.

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Published

06-01-2015

How to Cite

Goudar, M. A., J. H., M. K. M., S. R. A., H. P. V., and S. H. A. “ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITE FROM HABENARIA INTERMEDIA D. DON FOR SCREENING OF HEPATOPROTECITVE POTENTIAL AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY IN ALBINO RAT LIVER”. International Journal of Current Pharmaceutical Research, vol. 7, no. 1, Jan. 2015, pp. 57-61, https://journals.innovareacademics.in/index.php/ijcpr/article/view/4722.

Issue

Vol 7, Issue 1, 2015

Section

Original Article(s)