ISOLATION AND CHARACTERIZATION OF SECONDARY METABOLITE FROM HABENARIA INTERMEDIA D. DON FOR SCREENING OF HEPATOPROTECITVE POTENTIAL AGAINST CARBON TETRACHLORIDE INDUCED TOXICITY IN ALBINO RAT LIVER
Keywords:Habenaria intermedia, Scopoletin, Hepatoprotecitve, CCl4, Silymarin
The purpose of the study was to isolate the coumarin glycoside constituent from the ethyl acetate fraction of tubers of Habenaria intermedia D. Don by column chromatography using the gradient elution technique. The isolated coumarin glycoside was characterized by spectral studies and screened for hepatoprotecitve potential on CCl4 induced toxicity in rat liver. The coumarin glycoside (Scopoletin) protective activity was evaluated by the assay of liver function biochemical parameters such as SGOT, SGPT, Bilirubin, total protein and histopathological studies of the liver. Results showed that the toxic effect of CCl4 was controlled significantly by restoration of the levels of serum bilirubin, proteins and enzymes as compared to the normal and standard drug Silymarin treated groups. Histology of the liver sections of the rats treated with isolated coumarin glycoside showed the presence of normal hepatic cords, absence of necrosis and fatty infiltration, which further substantiated hepatoprotecitve activity. Therefore, outcome of the present study ascertains that the isolated coumarin glycoside possesses significant hepatoprotecitve activity.
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