• PALLAVI CHALIVENDRA Pharmacology, SVIMS, Sri Padmavathi Medical College for Women, Tirupati
  • RAVINDRA KUMAR GANJIKUNTA Pharmacology, SVIMS, Sri Padmavathi Medical College for Women, Tirupati
  • UMAMAHESWARA RAO K. Pharmacology, SVIMS, Sri Padmavathi Medical College for Women, Tirupati
  • RI PHANI TEJASWI PULLAKANAM Gayatri Vidya Parishad Institute of Health Care and Medical Technology, Marikavalasa, Visakhapatnam, Andhra Pradesh, India



Antimicrobial activity, Plant extracts, Methanol, Microorganisms


Objective: Herbal medicine is still the mainstay of about 75-80% of the whole population, and the major part of traditional therapy involves the use of plant extract and their active constituents against infectious pathogens. Objective: The study aimed to investigate in vitro antibacterial activity of extracts from some medicinal plants against the most common microbial pathogens including MDR bacteria.

Methods: The processing of plant materials was performed with the washing, drying and grinding of collected plant materials. The plant extracts were prepared by mixing 10 g of powder to 150 ml of ethanol solvent for 5 h at room temperature and sonicated for 15 min; for prepared test samples under laboratory conditions, the air-dried samples were mixed with the respective solvent (1:15 w/v) for 72 h at room temperature with occasional and then filtered through Whattman filter paper No.1. The obtained extract was freed from the solvent by evaporation under reduced pressure and then resuspended in the appropriate solvent to make the solution of known concentration of 10-50 mg/ml. The extract was stored at 4 °C in airtight glass bottle for the antibacterial assay using the Agar-well diffusion method. Ciprofloxacin was used as a control antibiotic.

Results: The growth of K. pneumoniae, Proteus mirabilis, P. aeruginosa and E. coli were inhibited better with the plant extract Tinospora cardifolia leaves than ciprofloxacin antibiotic. Coagulase-negative Staphylococci was inhibited greatly with Costus igneus leaf extract. The growth of Enterococcus faecalic was inhibited significantly with Tridax procumbens leaf extract than ciprofloxacin.

Conclusion: The present study indicates Tridax procumbens, T. cordifolia and Costus igneus methanolic leaf extracts were showed strong antimicrobial activity against all the tested cultures. They were rich in primary and secondary constituents. Most of the biologically active phytochemicals were present in methanolic extract. The tested plant extracts were more efficient than standard antibiotic ciprofloxacin used in the current study.


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Chikezie PC. Herbal medicine: yesterday, today and tomorrow. Altern Integr Med 2015;4(3)1-5. doi: 10.4172/2327-5162.1000195.

Gupta BM, Ahmed KKM, Gupta R. Global research on T. cordifolia (Medicinal plant) with special reference to India. A scientometric assessment publication output during 2001-2016. Int J Pharmacognacy Chin Med. 2018;2(4):000141.

Ahmad I, Mehmood Z, Mohammad F. Screening of some Indian medicinal plants for their antimicrobial properties. J Ethnopharmacol. 1998;62(2):183-93. doi: 10.1016/s0378-8741(98)00055-5, PMID 9741890.

Gonelimali FD, Lin J, Miao W, Xuan J, Charles F, Chen M. Antimicrobial properties and mechanism of action of some plant extracts against food pathogens and spoilage microorganisms. Front Microbiol. 2018 Jul 24;9:1639. doi: 10.3389/fmicb.2018.01639, PMID 30087662, PMCID PMC6066648.

Gupta RN, Viswas K, Pathak M, Singh PS, Gupta A. Antibacterial activities of ethanolic extracts of plants used in folk medicine. Int J Res Ayurveda Pharm. 2010;1(2):529-35.

Orhan DD, Ozcelik B. Hobaay S, Vural M. Assessment of antioxidant, antibacterial, antimycobacterial, and antifungal activities of some plants used as folk remedies in Turkey against dermatophytes and yeast-like fungi. Turk J Biol. 2012;36:672-86.

Jamal BS, Mohammed IA. Bacterial isolates from wound infections and their antibiotic susceptibility pattern in Kassala teaching hospital, Sudan. Am J Microbiol Res. 2019;7(4):102-7.

Mummed B, Abraha A, Feyera T, Nigusse A, Assefa S. In vitro antibacterial activity of selected medicinal plants in the traditional treatment of skin and wound infections in eastern Ethiopia. BioMed Res Int. 2018;2018:1862401. doi: 10.1155/2018/1862401, PMID 30079345.

Mariana CG, Carlos HGM, Jaqueline M, Tais MB, Luis VSS. Phytochemical screening of Azadirachta indica A. Juss for antimicrobial activity. Afr J Microbiol Res. 2017;11(4):117-22. doi: 10.5897/AJMR2016.8337.

Begashaw B, Mishra B, Tsegaw A, Shewamene Z. Methanol leaves extract Hibiscus micranthus Linn exhibited antibacterial and wound healing activities. BMC Complement Altern Med. 2017;17(1):337. doi: 10.1186/s12906-017-1841-x, PMID 28651570.

Geethanjali B, Avinash KO, Kumar JR, Chandrashekrappa GK, Dinesh Kumar V, Kanthesh M. Basalingapp. Tinospora cordifolia: the antimicrobial property of the leaves of amruthaballi. J Bacteriol Mycol Open Access. 2017;5(5):363-71.

Cappelletty D. Microbiology of bacterial respiratory infections. Pediatr Infect Dis J. 1998;17(8);Suppl:S55-61. doi: 10.1097/00006454-199808001-00002, PMID 9727651.

Hu SH, Wang JC, Kung HF, Wang JT, Lee WL, Yang YH. Antimicrobial effect of extracts of cruciferous vegetables. Kaohsiung J Med Sci. 2004;20(12):591-9. doi: 10.1016/S1607-551X(09)70264-5. PMID 15696789.

Owis AI. Broccoli; the green beauty: a review. J Pharm Sci Res. 2015;7:696-703.

Pal SK, Mukherjee PK, Saha K, Pal M, Saha BP. Antimicrobial action of the leaf extract of Moringa oleifera lam. Anc Sci Life. 1995 Jan;14(3):197-9. PMID 22556699, PMCID PMC3331240.

Syed A, Benit N, Alyousef AA, Alqasim A, Arshad M. In vitro antibacterial, antioxidant potentials and cytotoxic activity of the leaves of tridax procumbens. Saudi J Biol Sci. 2020 Feb;27(2):757-61. doi: 10.1016/j.sjbs.2019.12.031. PMID 32210697, PMCID PMC6997847.

Hegde PK, Rao HA, Rao PN. A review on insulin plant (Costus igneus Nak). Pharmacogn Rev. 2014 Jan;8(15):67-72. doi: 10.4103/0973-7847.125536, PMID 24600198, PMCID PMC3931203.

Gothandam KM, Aishwarya R, Karthikeyan S. Preliminary screening of antimicrobi al properties of few medicinal plants. J Phytol. 2010;2:1-6.

SarNaswathi R, Upadhyay L, Venkatakrishnan R, Meera R, Devi P. Isolation and biological evaluation of steroid from the stem of Costus igneus. J Chem Pharm Res. 2010;2:444-8.



How to Cite

CHALIVENDRA, P., R. K. GANJIKUNTA, U. . RAO K., and R. P. T. PULLAKANAM. “IN VITRO ASSESSMENT OF NATURAL HERBAL EXTRACTS FOR ANTIMICROBIAL ACTIVITY”. International Journal of Current Pharmaceutical Research, vol. 15, no. 2, Mar. 2023, pp. 22-25, doi:10.22159/ijcpr.2023v15i2.2079.



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