IN VITRO EVALUATION OF ANTHELMINTIC ACTIVITY OF ETHANOLIC EXTRACT OF AERIAL PARTS OF PHYLLANTHUS FRATERNUSWEB. IN ADULT EARTHWORMS

ASHEM DHANACHANDRA SINGH; MOIRANGTHEM SOBINA; CHINGTHAM KANCHAN; PAONAM SHYAMASAKHI DEVI; NAMEIRAKPAM MEENA DEVI

doi:10.22159/ijcpr.2023v15i2.2089

Authors

ASHEM DHANACHANDRA SINGH Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur
MOIRANGTHEM SOBINA Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur
CHINGTHAM KANCHAN Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur
PAONAM SHYAMASAKHI DEVI Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur
NAMEIRAKPAM MEENA DEVI Department of Pharmacology, Regional Institute of Medical Sciences, Imphal, Manipur

DOI:

https://doi.org/10.22159/ijcpr.2023v15i2.2089

Keywords:

Helminthiasis, Phyllanthus fraternus Web, Pheretimaposthuma, Albendazole, Anthelmintic

Abstract

Objective: The present study aimed to evaluate the anthelmintic activity of ethanolic extract of aerial part of Phyllanthus fraternus Web. leaves (EEPF) using adult earthworms (Pheretimaposthuma).

Methods: Adult earthworms (24in number) were divided into 4 groups with 6 worms in each group (n=6). The anthelmintic activity of EEPF at two different doses (25 mg/ml and 50 mg/ml) were evaluated by assessing the time of paralysis (min) and time of death (min) of the earthworms. Albendazole (25 mg/ml) was used as standard and 2% gum acacia as control.

Results: Data were analyzed statistically by ANOVA followed by Bonferroni test. P<0.05 was considered as significant. The result showed that EEPF had significant anthelmintic activity (p<0.001) but less potent than the standard drug albendazole.

Conclusion: EEPF demonstrated significant anthelmintic activity but less potent than the standard drug albendazole. However, further studies with higher doses are required to evaluate the dose-dependent activity and to evaluate the exact mechanism responsible for anthelmintic activity.

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