CULTURE-DEPENDENT APPROACHES AMONG THE CARIES PATHOGENS ISOLATED FROM THE CARIOUS DENTINE AND THEIR EMERGING DRUG RESISTANCE

RUCHITA S. LOHIYA; NAGA NIMISHA INDUGU; VIJAYSHRI DEOTALE

doi:10.22159/ijcpr.2023v15i5.3055

Authors

RUCHITA S. LOHIYA Department of Microbiology, MGIMS, Sewagram, Wardha-442102, Maharashtra, India
NAGA NIMISHA INDUGU Department of Microbiology, MGIMS, Sewagram, Wardha-442102, Maharashtra, India
VIJAYSHRI DEOTALE Department of Microbiology, MGIMS, Sewagram, Wardha-442102, Maharashtra, India

DOI:

https://doi.org/10.22159/ijcpr.2023v15i5.3055

Keywords:

Dental caries, Drug resistance, Pathogens, Antimicrobial susceptibility, Microbial cultute

Abstract

Objective: To evaluate the commonest caries pathogens from different type of caries andto screen for the emergence of drug resistance among the caries causing pathogenic bacteria.

Methods: A cross-sectional study was conducted in the department of Microbiology. Sample Size was 44 and samples from carious dentine which was immediately transported to the microbiology laboratory. Samples were processed microbiologically to isolate the caries pathogens. Identification of strains were done by standard biochemical characterization studies. The isolates were subjected to antimicrobial sensitivity test. The results were recorded and analyzed for drug resistance.

Results: Out of 44 patients, 40 aerobic bacteria, 2 anaerobic bacteria and 2 fungi were isolated. Out of the 40 aerobic bacteria, the most common isolated was Klebsiella pneumoniae followed by Pseudomonas spp. and Streptococcus viridians. Ciprofloxacin, Gentamycin and Linezolid showed excellent activity against Gram-positive Bacteria. The most frequently involved teeth of dental caries were mandibular 1st molar (54.54%) followed by Mandibular 2^nd molar (13.63%).

Conclusion: In our study we found that Klebsiella pneumoniae, Pseudomonas sp., Streptococcus viridians were the most frequent organisms encountered. The study implicates the need for time to time antimicrobial susceptibility examination of the dental caries pathogens that will help to prevent the emergence of resistance property among the dentinal pathogenic organisms.

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References

Van Ruyven FO, Lingstrom P, Van Houte J, Kent R. Relationship among mutans streptococci, “low-pH” bacteria, and lodophilic polysaccharide-producing bacteria in dental plaque and early enamel caries in humans. J Dent Res. 2000;79(2):778-84. doi: 10.1177/00220345000790021201, PMID 10728980.

Love RM, Jenkinson HF. Invasion of dentinal tubules by oral bacteria. Crit Rev Oral Biol Med. 2002;13(2):171-83. doi: 10.1177/154411130201300207, PMID 12097359.

Llena Puy MC, Montanana Llorens C, Forner Navarro L. L Cariogenic oral flora and its relation to dental caries. ASDC J Dent Child. 2000;67(1):42-6. PMID 10736657.

Hoshino E. Predominant obligate anaerobes in human carious dentin. J Dent Res. 1985;64(10):1195-8. doi: 10.1177/00220345850640100301, PMID 3861648.

Nagaoka S, Miyazaki Y, Liu HJ, Iwamoto Y, Kitano M, Kawagoe M. Bacterial invasion into dentinal tubules of human vital and nonvital teeth. J Endod. 1995;21(2):70-3. doi: 10.1016/S0099-2399(06)81098-8.

Martin FE, Nadkarni MA, Jacques NA, Hunter N. Quantitative microbiological study of human carious dentine by culture and real-time PCR: association of anaerobes with histopathological changes in chronic pulpitis. J Clin Microbiol. 2002;40(5):1698-704. doi: 10.1128/JCM.40.5.1698-1704.2002, PMID 11980945.

Brailsford SR, Tregaskis RB, Leftwich HS, Beighton D. The predominant Actinomyces spp. isolated from infected dentin of active root caries lesions. J Dent Res. 1999;78(9):1525-34. doi: 10.1177/00220345990780090701, PMID 10512387.

Ferrari PH, Cai S, Bombana AC. Effect of endodontic procedures on Enterococci, enteric bacteria and yeasts in primary endodontic infections. Int Endod J. 2005;38(6):372-80. doi: 10.1111/j.1365-2591.2005.00947.x, PMID 15910472.

Smiline GAS, Pandi Subak, Hariprasad P, Raguraman R. A preliminary study on the screening of emerging drug resistance among the caries pathogens isolated from carious dentine. Indian J Dent Res. 2012;23(1):26-30. doi: 10.4103/0970-9290.99033, PMID 22842245.

Ozaki K, Matsuo T, Nakae H, Noiri Y, Yoshiyama M, Ebisu S. A quantitative comparison of selected bacteria in human carious dentine by microscopic counts. Caries Res. 1994;28(3):137-45. doi: 10.1159/000261635, PMID 8033185.

Benson HJ. Microbiological applications: laboratory manual in general microbiology. McGraw-Hill Publication; 2004.

Bauer AW, Kirby WM, Sherris JC, Turck M. Antibiotic susceptibility testing by a standardized single disk method. Am J Clin Pathol. 1966;45(4):493-6. doi: 10.1093/ajcp/45.4_ts.493, PMID 5325707.

Keyes PH. Research in dental caries. J Am Dent Assoc. 1968;76(6):1357-73. doi: 10.14219/jada.archive.1968.0186, PMID 4870825.

Kleinberg I. A mixed-bacteria ecological approach to understanding the role of the oral bacteria in dental caries causation: an alternative to streptococcus mutans and the specific-plaque hypothesis. Crit Rev Oral Biol Med. 2002;13(2):108-25. doi: 10.1177/154411130201300202, PMID 12097354.

Mathew GC, Ranganathan LK, Gandhi S, Jacob ME, Singh I, Solanki M. Odontogenic maxillofacial space infections at a tertiary care center in North India: a five-year retrospective study. Int J Infect Dis. 2012;16(4):e296-302. doi: 10.1016/j.ijid.2011.12.014, PMID 22365137.

Fating NS, Saikrishna D, Vijay Kumar GS, Shetty SK, Raghavendra Rao M. Detection of bacterial flora in orofacial space infections and their antibiotic sensitivity profile. J Maxillofac Oral Surg. 2014;13(4):525-32. doi: 10.1007/s12663-013-0575-7, PMID 26225023.

Flynn TR, Shanti RM, Levi MH, Adamo AK, Kraut RA, Trieger N. Severe odontogenic infections, part 1: prospective report. J Oral Maxillofac Surg. 2006;64(7):1093-103. doi: 10.1016/j.joms.2006.03.015, PMID 16781343.

Huang TT, Tseng FY, Liu TC, Hsu CJ, Chen YS. Deep neck infection in diabetic patients: comparison of clinical picture and outcomes with nondiabetic patients. Otolaryngol Head Neck Surg. 2005;132(6):943-7. doi: 10.1016/j.otohns.2005.01.035, PMID 15944569.

Shakya N, Sharma D, Newaskar V, Agrawal D, Shrivastava S, Yadav R. Epidemiology, microbiology and antibiotic sensitivity of odontogenic space infections in central India. J Maxillofac Oral Surg. 2018 Sep;17(3):324-31. doi: 10.1007/s12663-017-1014-y, PMID 30034150.

Poeschl PW, Spusta L, Russmueller G, Seemann R, Hirschl A, Poeschl E. Antibiotic susceptibility and resistance of the odontogenic microbiological spectrum and its clinical impact on severe deep space head and neck infections. Oral Surg Oral Med Oral Pathol Oral Radiol Endod. 2010;110(2):151-6. doi: 10.1016/j.tripleo.2009.12.039, PMID 20346713.

Gill Y, Scully C. Orofacial odontogenic infections: review of microbiology and current treatment. Oral Surg Oral Med Oral Pathol. 1990;70(2):155-8. doi: 10.1016/0030-4220(90)90109-6, PMID 2290641.

Singh M, Kambalimath DH, Gupta KC. Management of odontogenic space infection with microbiology study. J Maxillofac Oral Surg. 2014;13(2):133-9. doi: 10.1007/s12663-012-0463-6, PMID 24822004.

Heimdahl A, von Konow L, Satoh T, Nord CE. Clinical appearance of orofacial infections of odontogenic origin in relation to microbiological findings. J Clin Microbiol. 1985;22(2):299-302. doi: 10.1128/jcm.22.2.299-302.1985, PMID 4031041.

Moenning JE, Nelson CL, Kohler RB. The microbiology and chemotherapy of odontogenic infections. J Oral Maxillofac Surg. 1989;47(9):976-85. doi: 10.1016/0278-2391(89)90383-2.

Storoe W, Haug RH, Lillich TT. The changing face of odontogenic infections. J Oral Maxillofac Surg. 2001;59(7):739-48. doi: 10.1053/joms.2001.24285, PMID 11429732.

Anderson MH, Shi W. A probiotic approach to caries management. Pediatr Dent. 2006;28(2):151-3. PMID 16708790.

Uwaezuoke JC, Aririatu LE. A survey of antibiotic resistant staphylococcus aureus strains from clinical sources in owerri. J Appl Sci Environ Manag. 2004;8(1):67-9. doi: 10.4314/jasem.v8i1.17230.

Dwivedi D, Kushwah T, Kushwah M, Singh V. Antibiotic susceptibility pattern against pathogenic bacteria causing Dental Caries South Asian. J Exp Biol. 2011;1(1):31-5.

Kaur N, Sahni P, Singhvi A, Hans MK, Ahluwalia AS. Screening the drug resistance property among aerobic pathogenic microorganisms of dental caries in North-Western Indian population: a preliminary study. J Clin Diagn Res. 2015;9(7):ZC05-8. doi: 10.7860/JCDR/2015/11989.6143, PMID 26393195.

Coelho ED, Arrais JP, Matos S, C Pereira C, N Rosa N, MJ Correia MJ. Computational prediction of the human-microbial oral interactome. BMC Systems Biology. 2014 ;8:24-1624. doi: 10.1186/1752-0509-8-24, PMID 24576332.