ANTICONVULSANT EFFECT OFBERBERIS ARISTATA ROOT EXTRACT IN MICE
DOI:
https://doi.org/10.22159/ijcpr.2023v15i6.3094Keywords:
Anticonvulsant, Berberine, Maximal electroshock seizure, Pentylenetetrazole seizureAbstract
Objective: Berberine is an isoquinolone alkaloid present in many plants and reported to possess anti-diabetic, hypolipidemic, anti-cancer, anti-bacterial, anti-pyretic, antiviral and anti-inflammatory effects. In addition, berberine can modulate neurotransmitter like N-methyl-D-aspartate (NMDA), serotonin and Nitric Oxide, thus can produce anticonvulsant effect.
Methods: The seizures were induced in mice by maximal pentylenetetrazol and electroshock. The effect of root extract on seizures was compared with standard anticonvulsant agents, phenytoin and diazepam.
Results: Theroot extract of berberine aristata suppressed duration of tonic convulsions in maximal electroshock-induced seizures while it delayed time of onset of seizure as well as significantly decreased the duration of myoclonic-jerks-in pentylenetetrazol-induced seizures. Further, the study also indicated that the root extract of berberine aristata also produced motor impairment at the antiseizure doses.
Conclusion: The present study indicated that berberine exhibits anticonvulsant activity in both models and can be useful in epileptic patients.
Downloads
References
Imanshahidi M, Hosseinzadeh H. Pharmacological and therapeutic effects of Berberis vulgaris and its active constituent, berberine. Phytother Res. 2008;22(8):999-1012. doi: 10.1002/ptr.2399, PMID 18618524.
Beg S, Swain S, Hasan H, Barkat MA, Hussain MS. Systematic review of herbals as potential anti-inflammatory agents: recent advances, current clinical status and future perspectives. Pharmacogn Rev. 2011;5(10):120-37. doi: 10.4103/0973-7847.91102, PMID 22279370.
Rashmi, Rajasekaran A, Pokhriyal R, Rashmi, Singh Y. Quantitative estimation of berberine in roots of different provenances of berberis aristata DC by HPLC and study of their antifungal properties. Phcog Mag. 2009;5(20). doi: 10.4103/0973-1296.58566.
Rathi B, Sahu J, Koul S, Kosha RL. Detailed pharmacognostical studies on Berberis aristata DC plant. Anc Sci Life. 2013;32(4):234-40. doi: 10.4103/0257-7941.131981, PMID 24991073.
Isah T. Anticancer alkaloids from trees: development into drugs. Pharmacogn Rev. 2016;10(20):90-9. doi: 10.4103/0973-7847.194047, PMID 28082790.
Chakraborty R, Lobo R, Prabhu MM, Umakanth S, Chowdhury M. Retal berberine hydrochloride could prove to be a promising bullet against clostrium difficile infection: a preliminary study from south India. Asian J Pharm Clin Res. 2017;12:419-24.
Kulkarni SK, Dhir A. Berberine: a plant alkaloid with therapeutic potential for central nervous system disorders. Phytother Res. 2010;24(3):317-24. doi: 10.1002/ptr.2968, PMID 19998323.
Peng WH, Wu CR, Chen CS, Chen CF, Leu ZC, Hsieh MT. Anxiolytic effect of berberine on the exploratory activity of the mouse in two experimental anxiety models: interaction with drugs acting at 5-HT receptors. Life Sci. 2004;75(20):2451-62. doi: 10.1016/j.lfs.2004.04.032, PMID 15350820.
Köhling R. Voltage-gated sodium channels in epilepsy. Epilepsia. 2002;43(11):1278-95. doi: 10.1046/j.1528-1157.2002.40501.x, PMID 12423377.
Kwan P, Brodie MJ. Early identification of refractory epilepsy. N Engl J Med. 2000;342(5):314-9. doi: 10.1056/NEJM200002033420503, PMID 10660394.
Samren EB, van Duijn CM, Koch S, Hiilesmaa VK, Klepel H, Bardy AH. Maternal use of antiepileptic drugs and the risk of major congenital malformations: a joint European prospective study of human teratogenesis associated with maternal epilepsy. Epilepsia. 1997;38(9):981-90. doi: 10.1111/j.1528-1157.1997.tb01480.x, PMID 9579936.
Luszczki JJ, Czuczwar P, Cioczek Czuczwar A, Czuczwar SJ. Arachidonyl-2’-chloroethylamide, a highly selective cannabinoid. CB1 receptor agonist, enhances the anticonvulsant action of valproate in the mouse maximal electroshock-induced seizure model. Eur J Pharmacol. 2006;547:65e74.
Kulkarni SK, Dhir A. On the mechanism of antidepressant-like action of berberine chloride. Eur J Pharmacol 2008;589:163-72.
Kulkarni SK. Handbook of experimental pharmacology. 3rd ed. New Delhi: Vallabh Prakashan; 1999. p. 131-4.
Cartmell SM, Gelgor L, Mitchell D. A revised-rota rod procedure for measuring the effect of antinociceptive drugs on motor function in the rat. J Pharmacol Methods 1991;26:149-59.
Bhutada P, Mundhada Y, Bansod K, Dixit P, Umathe S, Mundhada D. Anticonvulsant activity of berberine, an iso-quinoline alkaloid in mice. Epilepsy Behav. 2010;18:207e210.
Hosseinzadeh H, Ramezani M, Shafaei H, Taghiabadi E. Anticonvulsant effect of berberis integerrima L. root extracts in mice. J Acupunct Meridian Stud. 2013;6(1):12-7.
Sharma K, Ranjan B, Neelam C, Birendra S, Kumar SN. Berberis Aristata. International Journal of Research in Ayurveda and Pharmacy. 2011;2:383-8.
Mokhber Dezfuli N, Saeidnia S, Gohari AR, Kurepaz Mahmoodabadi M. Phytochemistry and pharmacology of berberis species. Pharmacogn Rev. 2014;8:8-15.
Rang HP, Dale MM, Ritter JM, Moore PK. Pharmacology. 5th ed. Edinburgh: Churchill Livingstone; 2007. p. 550-61.
Yoo JH, Yang EM, Cho JH, Jeong SM, Nah SY, Kim HC. Inhibitory effects of berberine against morphine-induced locomotor sensitization and analgesic tolerance in mice. Neurosci. 2006;142:953–61.
Published
How to Cite
Issue
Section
Copyright (c) 2023 DIVYA GOEL
This work is licensed under a Creative Commons Attribution 4.0 International License.
