ANTIMICROBIAL ACTIVITY OF WITHANIA SOMNIFERA AND CALOTROPIS PROCERA ON PATHOGENIC STRAINS

Abstract

Objectives: The present study was planned to investigate the antimicrobial activity with Minimum Inhibitory Concentration of different part of Withania somnifera and Calotropis procera. Therefore, the preliminary successive solvent extraction and chemical test revealed the presence of secondary metabolites in various extracts, and provide us clue for further investigation.

Methods: Different solvents i.e., Chloroform, and Ethanol, were used for extraction of active secondary metabolites by Soxhlet method. Percentage of yield⁴, and MIC by gradient plate technique [13]. Statistical analysis done by the standard error by mean (SEM), in which n=3.

Results: The significant antimicrobial activity found in Calotropis procera stem extract with ethanol was 1.3±0.046 mg/ml to 2.8±0.009 mg/ml on bacterial strain and 10.4±0.013 mg/ml to 13.3±0.015 mg/ml MIC values with±SEM. In Withania somnifera, it was an average. The highest percentage of yield was observed in Withania Somnifera with Ethanol and Chloroform i. e from 2.78% to 4.83%. Various numbers of secondary metabolites were extracted from 3 to 5 in Withania somnifera and Calotropis procera combindly.

Conclusion: Ethanol has been shown as a best solvent to yield, extracted components and antimicrobial effects. In CRE and CSE extracts, only root extracts has been shown best inhibitory effects on all concerned microbes, while chloroform were also good solvents to extract secondary metabolite components.