THE PREBIOTIC INFLUENCE OF INULIN ON GROWTH RATE AND ANTIBIOTIC SENSITIVITY OF Lactobacillus casei
Abstract
Objective: This research study is focused on the prebiotic effect of inulin on the antibiotic sensitivity of probiotic Lactobacillus casei and on the determination of functionality of specific growth rate (µ) of the probiotic bacteria on the concentrations of lactose (CL= 10-30g/L) and inulin (CI = 0.164-0.625g/L) along with the optimization of growth condition through Response Surface Methodology.
Methods: The sensitivity of Lactobacillus casei towards norfloxacin was determined using well diffusion method. Using the initial values of µ (h-1) of  Lactobacillus casei at different values of CL(g/L) and CI (g/L), the functionality of µ on the concentrations of the carbon sources have been derived and optimum condition has been identified.
Results: Although L.casei is sensitive to norfloxacin, resistance is developed in presence of inulin. Quadratic model equation µ =0.83+0.054 * CL -0.035* CI -0.049* CL * CI -0.29* CL 2 -0.33* CI 2  is valid and the optimum value of specific growth rate is 0.8285h-1 at  CL=20g/L and CI =0.32g/L.                                                                         Â
Conclusion: The interesting observation of development of antibiotic resistance of L.casei in presence of inulin suggests that the intake of probiotic L casei, may be done along with prebiotic inulin when a patient is treated with antibiotics like norfloxacin. Moreover the model equation correlating the functionality of growth rate of L.casei on lactose and inulin will be helpful in fortifying the probiotic milk products and drugs with prebiotics like inulin.
Â
Â
References
Gaggìa, F., Mattarelli P., Biavati B. Probiotics and prebiotics in animal feeding for safe food production. International Journal of Food Microbiology, 2010; 141:S15–S28.
Saad, N., Delattre C., Urdaci M., Schmitter J.M., Bressollier P. An overview of the last advances in probiotic and prebiotic field. LWT - Food Science and Technology, 2013; 50: 1-16.
Kosin, B. and Rakshit S. K. Microbial and Processing Criteria for Production of probiotics : A Review. Food Technology Biotechnology, 2006; 44 (3): 371–379.
Manso, J., Luz M. M., Ya´n˜ez-Seden˜o P., Jose´ Pingarro´n M. Bienzyme amperometric biosensor using gold nanoparticle-modified electrodes for the determination of inulin in foods. Department of Analytical Chemistry, Faculty of Chemistry, University Complutense of Madrid, 28040 Madrid, Spain, 2007.
de Souza, O., Perego P., de Oliveira M. N., Converti A. Effect of inulin as prebiotic and synbiotic interactions between probiotics to improve fermented milk firmness. Jounal of Food Engineering, 2011; 107:36–40.
Azza, A., Abou-Arab, Hala A.T., Ferial M., Salem A. Physico-chemical properties of inulin produced from jerusalem artichoke tubers on bench and pilot plant scale. Australian Journal of Basic and Applied Sciences, 2011; 5(5): 1297-1309.
Toneli, J. T. C. L. , Park K. J., Ramalho J. R. P., Murr F. E. X. and Fabbro I. M. D. Rheological characterization of chicory root (cichorium intybusl.) inulin solution. Brazilian Jounal of Chemical Engineering, 2008; Vol. 25: No. 03: pp. 461 - 471.
Ashraf, R. and Shah N. P. Antibiotic resistance of probiotic organisms and safety of probiotic dairy products. International Food Research Jounal , 2011;18(3): 837-853.
Nelson, D. L. and Cox M. M. Lehninger. Principles of Biochemistry.pp. 2004;778-780.
Ricca, E., Calabrò V., Curcio S., Iorio G. Fructose production by chicory inulin enzyme hydrolysis: Kinetic study and reaction mechanism. Process Biochemistry, 2009; 44: 466–471.