EFFECT OF Solanum villosum (Mill.) EXTRACT AND ITS SILVER NANOPARTICLES ON HEMOPOIETIC SYSTEM OF DEN INDUCED HCC IN RATS

Abstract

ABSTRACT

Objective: This study aims to elucidate the different forms of Solanum villosum, ethanolic leaf extract of Solanum villosum (EESV) and Solanum villosum silver nanoparticles (SV-AgNPs) on hematological parameters in diethylnitrosamine (DEN) induced hepatocellular carcinoma in rats. Materials and Methods: Thirty male albino wistar rats were randomly assigned into five groups of six rats each. Group 1 was control, while groups 2 (DEN control), group 3 (EESV 200 mg / kg b.w), group 4 (SV-AgNPs 100 µg / kg b.w i.p), group 5 (Cyclophosphamide 50 mg / kg. b.w), orally for 16 weeks, respectively. Rats in all six groups received normal pallet diet and drinking water ad libitum. Complete blood count was done using SYSMEX Xs – 800 i automatic hematology analyzer.

Results and Discussion: Rats treated with DEN showed severe toxic symptoms and significant decrease in (P < 0.05) Hb, PCV, RBC, MCV, MCH, MCHC and platelets levels except white blood cell counts (WBC). After, the administration of ethanolic extract of Solanum villosum (EESV) and Solanum villosum silver nanoparticles (SV-AgNPs) showed a protective effect on hemopoietic levels of DEN induced rats.

Conclusion: Thus it is concluded that oral administration of ethanolic extract of Solanum villosum (EESV) and Solanum villosum silver nanoparticles (SV-AgNPs) caused an increased in the level of Hb concentration, PCV, RBC, MCV, MCH, MCHC and platelets. Whereas level of white blood cells was reduced in DEN induced hepatocellular carcinoma. 

 

Keywords:  Ethanolic extract of Solanum villosum (EESV), Solanum villosum silver nanoparticles (SV-AgNPs), diethylnitrosamine (DEN), white blood cells.