ANTIBACTERIAL ACTIVITY OF ENDEMIC BALLOTA NIGRA SUBSP. ANATOLICA AGAINST SOME HUMAN EYE PATHOGENS FROM TURKEY
Abstract
ABSTRACT Objective: The ethanol extracts obtained from the leaves of Ballota nigra subsp. anatolica P.H. Davis (Lamiaceae) were evaluated for their antibacterial activity against some human eye pathogens. Methods: Hospital isolates of Bacillus cereus, Pseudomonas aeruginosa, Klebsiella pneumoniae, Staphylococcus capitis, S. aureus, Staphylococcus epidermidis, Moraxella nonliquefaciens, and Propionibacterium acnes were obtained. Antibacterial activity was determined in the extracts using disc diffusion method. Some antibacterial antibiotics were used as a positive reference standard to determine the sensitivity of the isolates. Results: The extracts showed strong antibacterial activity against M. nonliquefaciens and P. acnes, with inhibition zones of 18.2 mm and 19.2 mm, respectively. The extracts also exhibited moderate activity against the other isolates. Conclusion: The results demonstrate that the leaf extract of the plant has significant activity and suggest that it may be useful in the treatment of eye infections. Keywords: Ballota nigra subsp. anatolica, Antibacterial activity, Eye pathogens.
References
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Pathogens EtOH extract (50 µg/mL) Diamater of zone of inhibition (mm)*
Standards
P (10 µg/disc) AMP (20 µg/disc) TOB (10 µg/disc)
B. cereus 11.4 15.4 12.8 17.4
P. aeruginosa 12.8 11.2 12.2 13.6
K. pneumoniae 15.8 19.0 16.4 17.2
S. capitis 14.6 17.8 20.2 19.4
S. aureus 14.2 21.4 16.8 12.2
S. epidermidis 16.4 18.6 19.2 14.8
M. nonliquefaciens 18.2 15.2 17.4 16.4
P. acnes 19.2 16.2 14.8 18.8
B. cereus: Bacillus cereus, P. aeruginosa: Pseudomonas aeruginosa, K. pneumoniae: Klebsiella pneumoniae, K. pneumoniae: Klebsiella pneumoniae,
S. capitis: Staphylococcus capitis, S. aureus: Staphylococcus aureus, S. epidermidis: Staphylococcus epidermidis, M. nonliquefaciens: Moraxella nonliquefaciens,
P. acnes: Propionibacterium acnes, *Zone of inhibition, including the diameter of the filter disc (6.0 mm); mean value of three independent experiments;
P: Penicillin (10 μg/disc), AMP: Ampicillin (20 μg/disc), TOB: Tobramycin discs (10 μg/disc)
Innovare Journal of Life Sciences, Vol 5, Issue 2, 2017, 1-3
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