• Jyoti Bala Chauhan
  • Abhishek Kumar
  • Wethroe Kapfo


Objectives: The  present  investigation  attempts  to  study  the  antioxidant  and  antihemolytic  activity  of  ethyl  acetate  fraction  of  the  bilimbi  extract (BE)  and  determine  the  contributory  phytochemicals.

Methods: Fresh fruits of Averrhoa bilimbi were dried and subjected to 60% aqueous methanol extraction followed by biphasic extraction with ethyl acetate and water. The ethyl acetate fraction (BE) underwent phytochemical screening, analyzed using RP- HPLC and 2,2-azino-bis(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS.+) radical scavenging activity, hydroxyl radical scavenging activity, RBC protection activity and DNA protection activity.

Results: In phytochemical screening analysis, we detected lipids while alkaloids, flavanoids, saponins, terpenoids, cardiac glycosides, reducing sugars and amino acids were not detected. HPLC analysis showed prominent peaks at 23, 27 and 37 min under 310 nm. The fraction expressed ABTS.+ radical scavenging activity,  hydroxyl radical scavenging activity, RBC protection activity and DNA protection activity wherein TEAC value was 11.5 µM, IC50 value of hydroxyl RSA and antihemolytic activity were 49 and 47 µg/ml respectively.

Conclusion: The ethyl acetate fraction of bilimbi predominantly comprised of lipids which exhibited significant antioxidant and protective properties.


Key words: Antioxidant, DNA protection activity, antihemolytic activity



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How to Cite

Chauhan, J. B., Kumar, A., & Kapfo, W. (2018). ANTIOXIDANT AND ANTIHEMOLYTIC ACTIVITY OF AVERRHOA BILIMBI EXTRACT. Innovare Journal of Life Sciences, 6(2), 5–7. Retrieved from



Original Article(s)