PREPARATION OF CHITOSAN STABILIZED OFLOXACIN- GOLD NANO CONJUGATE FOR THE IMPROVED ANTI BACTERIAL ACTIVITY AGAINST HUMAN PATHOGENIC BACTERIA
Abstract
Objective .In the present study, biocom[patible polymer chitosan  stabilized ofloxacin- gold nanoconjugate was  synthesized for the improved anti bacterial activity against clinical isolate of E.coli and Staphylococcus aureus
Methods. Chitosan stabilized oflaxacin-gold nanoparticles conjugate was prepared with aqueous solution of ofloxacin ,gold nanoparticles suspension and chitosan under optimum condition. Synthesized nanoconjugate was .characterized by scanning electron microscopy, FTIR and the synthesized nanoconjugate was tested against E.coli and Staph.aureus adopting well diffusion assay.
Result. Synthesis of chitosan stabilized ofloxacin- gold nanoconjugate was primarly confirmed by colour change of the reaction mixture, characteristic change in the FTIR pattern and size and shape by SEM. Anti bacterial activity revealed increased spectrum of antibacterial activity was recorded in chitosan stabilized ofloxacin - gold nanoconjugate against both the tested strains. The highest increase in inhibitory zone for E.coli and Staph.aureus was observed.
Conclusion. The present study suggests possible utilization of antibiotics –gold nanoconjugate as an effective anti microbial agent against the pathogenic bacteria
Key words. chitosan,ofloxacin,nanoconjugate ,E.coli, Staphylococcus aureus
References
Raghunath D. 2008, Emerging antibiotic resistance in bacteria with special reference to India; J. Biosci. 33(4), 593–603
. Venubabu Thati, Aashis S Roy, Ambika Prasad M V N, Shivannavar C T, Gaddad S M 2010.Nanostructured zinc oxide enhances the activity of antibiotics against Staphylococcus aureus. J. Biosci Tech, 1(2); 64â€69
. Karthick Raja Namasivayam S, Chandrasekar S, Savitha V. 2010,A first report of antifungal effect of butanol cell free extract of Streptomyces griseoaureofaciens, Journal of Pharmacy Research 3(9); 2188-2189
. Guy Applerot, Anat Lipovsky, Rachel Dror, Nina Perkas, Yeshayahu Nitzan,Rachel Lubart, and Aharon Gedanken, 2009, Adv. Funct. Mater. 19; 842–852
. Lee, C., J. Y. Kim, W. I. Lee, K. L. Nelson, J. Yoon, and D. L. Sedlak. 2008. Bactericidal effect of zero-valent iron nanoparticles on Escherichia coli. Environ. Sci. Technol. 42:4927–4933.
. Ahmad Z, Pandey R, Sharma S, Khuller G K. Alginate nanoparticles as antituberculosis drug carriers: formulation development, pharmacokinetics and therapeutic potential. Ind J Chest Dis Allied Sci 2005;48:171–6.
.Albrecht MA, Evan CW, Raston CL. Green chemistry and the health implications of nanoparticles. Green Chem 2006;8:417–32.
.Amir H. Faraji, Peter Wipf. Nanoparticles in cellular drug delivery. Bioorganic & Medicinal Chemistry 2009;17:2950–2962.
.Brooking, J., Davis, S. S., and Illum, L. Transport of nanoparticles across the rat nasal mucosa, J. Drug Target 9, 2001, 267–279.
. Gong P, Li H, He X, Wang K, Hu J, Tan W, et al. Preparation and antibacterial activity of Fe3O4@Ag nanoparticles. Nanotechnology 2007;18:604–11.
.Grainger.D.W, Castner.D.G, Nanobiomaterials and nanoanalysis: opportunities for improving the science to benefit biomedical technologies, Adv. Mater. 20, 2008, 867–877.
.Gu H, Ho PL, Tong E,Wang L, Xu B. Presenting vancomycin on nanoparticles to enhance antimicrobial activities. Nano Lett 2003;3(9):1261–3.
.Guzmán Maribel.G, Jean Dille, Stephan Godet, Synthesis of silver nanoparticles by chemical reduction method and their antibacterial activity, Proceedings of World Academy of Science, Engineering And Technology, 33, September 2008, 2070-3740.
. Karthick Raja Namasivayam.S, Gnanendra Kumar E and Reepika R. Synthesis of silver nanoparticles by Lactobaciluus acidophilus 01 strain and evaluation of its in vitro genomic DNA toxicity. Nano-Micro Lett. 2, 160-163 (2010).
..Karthick Raja Namasivayam, S. Ganesh and Avimanyu. Evaluation of anti-
bacterial activity of silver nanoparticles synthesized from Candida glabrata and Fusarium oxysporum. Int J Med Res. 2011; 1(3):131-136.
. Burygin. G. L, Khlebtsov. B. N, Shantrokha Dykman. A. N, L. A, Bogatyrev V. A and Khlebtsov. N. G. On the Enhanced Antibacterial Activity of Antibiotics Mixed with Gold Nanoparticles. Nanoscale Res Lett (2009) 4:794–801.
Lifeng Q, Zirong X, Xia Jiang, Caihong Hu and Xiangfei Zou. Preparation and antibacterial activity of chitosan nanoparticles. Carbohydrate Research 339 (2004) 2693–2700.
.Li, Zhi; Zhuang, Xu Pin; Liu, Xiao Fei; Guan, Yun Lin; Yao, Kang De. Polymer 2002, 43, 1541–1547.