The ameliorating effect of uncooked beans diet in CD-1 mice


  • Aduema W Department of Physiology,Gregory Univerity,Uturu,Abia State,Nigeria
  • Agbai JU Anatomy Dept,Abia State University,Uturu,Abia State
  • Wariso AC Dept of Physiology,Abia State,University



This study was therefore, designed to investigate the ameliorating effect of uncooked beans on pain sensation using three groups of Swiss white mice (control and test) weighing 20g-30g (n=10 each).The control group received normal rodent chow, while the test group received 15g of uncooked beans in 15g of rodent chow per day and serotonin precursor (5HTP) (0.2mg/15g w/w) diet. Water was given adlibitum while daily food and water intake, as well as body weight changes, were monitored during the 30-day study. The formalin tests were used to assess pain sensation. The results showed that in the formalin test, the frequencies and durations of paw licks and paw attention in both phases of the test was significantly lower (P< 0.05) compared to the control group. Therefore, consumption of uncooked Nigeran beans diet decreases pain sensation in mice.     

Author Biographies

Aduema W, Department of Physiology,Gregory Univerity,Uturu,Abia State,Nigeria

lecturer 11

Agbai JU, Anatomy Dept,Abia State University,Uturu,Abia State

Anatomy,lecturer 11

Wariso AC, Dept of Physiology,Abia State,University

Dept of Physioogy,Senior lecturer


(1).Abbott, F.V., Franklin, K.B., Ludwick, R.J.and Melzack R.1981. Apparent lack of tolerance in the formalin test suggests a different mechanism for morphine analgesia in different types of pain. Pharmacol.Biochem.Behav, 15: 637-40.

(2).Adeyele, E.J.1995.Studies of Chemical Composition and functional properties of African Yambeans(SpensotylisStenoorpa) flour. Ph,D Thesis, Department of chemistry, Federal University of Technology, Akure, Ondo State, Nigeria.

(3).Dorria,E., Campion,B., Sparvoli,F., Tava,A. and Nielsin,E.2012.Antinutient components and metabolites with health implications in seeds of 10 bean (Phaseolus Vulgaris and PhaseolusLinutus) landraces cultivated on Southern Ltaly.Journal of Food Composition.Anal.26,72-80.

(4).Hong-XI and Song, F. L 2001. Activity of plant flavenoids against antibiotic resident bacteria. Phythother. Res. 15: 39 – 43.

(5).Ito, S., Okuda – Ashiteka, E. and Minami, T .2001. Central and Peripheral roles of prostaglandins in pain and their interactions with novel neuropathies Nociceptin and nocistatin. Neuroscience Research, 41 (4). 299 – 332.

(6).Lyimo, M., Mugula, J. and Elias, T .1992.Nutritive composition of broth from selected bean varieties cooked for various periods. J. Sci. Food Agric, 58(4):535-539.

(7).Mariani, John F.1999.The Encyclopedia of American Food and drink. NY, Lebhar-Friendman, Books, 380.p

(8).Muller, H. 1992. Hindu medicine, Baltimore: The John Hopkins Press.

(9).Osim, E.E .2008.Neurophysiology (pp.24-27).Calabar.University of Calabar Press.

(10).Osim, E.E .2012.Our consumables and our emotions. Faculty of Basic Medical Science, University of Calaber, lecture series, july11, 2012.

(11).Portas, C.M., Bjorvatn, B. and Ursin, R .2000.Progress in Neurobiology, 60(1), 13-35.

(12).Roefof, P.and Perkins, K. A .2004.Relationship between pain and fear. American Journal of Public Health; 70:420.

(13).Sembulingam, K. and Sembulingam, P.2010.Neurophysiology of pain. Essentials of Physiology (pp., 803-810).New Delhi: Jaypee Brothers, Medical Publishers.



How to Cite

W, A., JU, A., & AC, W. (2019). The ameliorating effect of uncooked beans diet in CD-1 mice. Innovare Journal of Medical Sciences, 7(2), 1–3.



Original Article(s)