PREVALENCE OF TSST PRODUCING COAGULASE-NEGATIVE STAPHYLOCOCCUS AUREUS IN WOUND SAMPLES AND CHARACTERIZATION OF MRSA AGAINST TEA EXTRACT

MOHANADOSS PONRAJ; MANICHAM MALATHI; VASUDEO ZAMBARE; RAMASAMY SUBBAIYA

doi:10.22159/ijms.2021.v9i5.42146

Authors

MOHANADOSS PONRAJ Department of Biological Sciences, School of Mathematics and Natural Sciences, The Copperbelt University, Riverside, Kitwe, Zambia.
MANICHAM MALATHI Department of Microbiology, Vivekanandha College of Arts and Science for Women, Namakkal, Tamil Nadu, India.
VASUDEO ZAMBARE Department of Research and Development, Om Biotechnologies, Nashik, Maharashtra India.
RAMASAMY SUBBAIYA Department of Biological Sciences, School of Mathematics and Natural Sciences, The Copperbelt University, Riverside, Kitwe, Zambia.

DOI:

https://doi.org/10.22159/ijms.2021.v9i5.42146

Keywords:

Wound samples, β-lactamase, Methicillin-resistant Staphylococcus aureus, Coagulase negative, Toxic shock syndrome toxin, Tea extract

Abstract

Objective: Methicillin-resistant Staphylococcus aureus (MRSA) is a potential pathogen for hospital-acquired infections. This study was conducted to determine the prevalence of MRSA using tea extract.

Methods: All S. aureus isolates obtained from wound samples were studied for antibiotic resistance pattern using 23 different antibiotics. Based on coagulase negative, S. aureus isolates were identified for toxic shock syndrome toxin (TSST) gene and analyzed using PCR method. The antibacterial activities of tea extract were tested against MRSA using agar well-diffusion method.

Results: A total of 100 wound samples were collected from hospital, where 75% of samples showed presence of S. aureus. About 100% resistance to cefoperazone, ampicillin, penicillin, rifampicin, novobiocin, and vancomycin antibiotics was observed. The isolates showed less resistance <50% toward chloramphenicol (30%), ciprofloxacin (25%), gentamycin (52%), amikacin (38%), and imipenem (33%). Twenty-five isolates were selected for MRSA characterization based on multiple drug resistance pattern. Coagulase-negative S. aureus isolates showed presence of TSST gene. Tea extract (2%) showed effective antibacterial activity against MRSA strains.

Conclusion: The study showed the presence of MRSA at higher level and suggesting to out further epidemiological study on such infections. However, cost-effective and easily available tea extract was found to be the best antimicrobial agent for preventing such bacterial infection and to reduce the risk of emerging resistance.

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