TREATMENT OF EXTRA-VIRGIN OLIVE OIL ON ETHANOL- AND BENZENE-INDUCED ACINAR CELLS DAMAGE IN ADULT WISTAR RATS

ADETUNJI OPEYEMI ADEBOLA; ADERUPOKO IDAYAT TOMILOLA; ADETUNJI OLUWASEYI ADEGOKE; OGUNBIYI BABAFEMI TOSIN; ADETUNJI IYABODE TOYIN; NWOBI NNENNA LINDA; OGUNBIYI OLUBUNMI ESTHER; NWOBI JOSEPH CHIGBOGU; BEJIDE AYODELE ROLAND

doi:10.22159/ijms.2022.v10i2.44088

Authors

ADETUNJI OPEYEMI ADEBOLA Department of Anatomy, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.
ADERUPOKO IDAYAT TOMILOLA Department of Anatomy, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.
ADETUNJI OLUWASEYI ADEGOKE Department of Biochemistry, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.
OGUNBIYI BABAFEMI TOSIN Department of Biochemistry, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.
ADETUNJI IYABODE TOYIN Department of Pure and Applied Biology, Ladoke Akintola University, Ogbomoso, Oyo State, Nigeria.
NWOBI NNENNA LINDA Department of Chemical Pathology, School of Basic Clinical Sciences, Babcock University, Ogun State, Nigeria.
OGUNBIYI OLUBUNMI ESTHER Department of Anatomy, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.
NWOBI JOSEPH CHIGBOGU Department of Biochemistry, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.a
BEJIDE AYODELE ROLAND Department of Anatomy, School of Basic Medical Sciences, Babcock University, Ogun State, Nigeria.

DOI:

https://doi.org/10.22159/ijms.2022.v10i2.44088

Keywords:

Ethanol, Benzene, Extra Virgin-Olive Oil, Acinar Cells

Abstract

Objective: The study is focused on the effect ethanol and benzene on the acinar cells and extra- virgin olive oil (EVOO) as an ameliorative agent.

Methods: Forty-eight Wistar rats were divided into eight groups of six animals per group: (1) Control group (a placebo of water), (2) EVOO, (3) 25% ethanol, (4) 200 mg/kg benzene, (5) 25% ethanol+200 mg/kg benzene (EB), (6) 25% ethanol+EVOO (EO), (7) 200 mg/kg benzene+EVOO (BO), and (8) 25% ethanol+200 mg/kg benzene+EVOO (EBO). The ethanol, benzene, and EVOO were administered orally.

Results: All the treatment groups lost body weight except Groups 1 and 2. The histology of the pancreas showed significant (p≤0.05) damage to the acinar cells of groups exposed to 25% ethanol, 200 mg/kg benzene, and EB when compared to the control, EVOO, EO, BO, and EBO. The enzymatic activities of amylase and lipase evaluated showed significant (p≤0.05) decrease in EO and BO as compared to 25% ethanol, 200 mg/kg benzene, and EB-treated groups.

Conclusion: There was an elevated glucose concentration in 25% ethanol and EBO as compared to control and EVOO has anti-inflammatory potential across the ameliorated groups.

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