EVALUATION OF ANTICATARACT ACTIVITY OF SCAEVOLA TACCADA ON GLUCOSE-INDUCED CATARACT IN GOAT LENS: AN EX VIVO APPROACH

GALATHIYA MEET; SAVANKUMAR CHHATROLA; BHARGAV KAMANI; TEJAS GANATRA

doi:10.22159/ijms.2024v12i6.52850

Authors

GALATHIYA MEET Department of Pharmacology, Anand Pharmacy College, Gujarat Technical University, Ahmedabad, Gujarat, India
SAVANKUMAR CHHATROLA Department of Pharmacology, School of Pharmacy, RK University, Rajkot, Gujarat, India
BHARGAV KAMANI Department of Pharmacology, School of Pharmacy, RK University, Rajkot, Gujarat, India
TEJAS GANATRA Department of Pharmacology, School of Pharmacy, RK University, Rajkot, Gujarat, India

DOI:

https://doi.org/10.22159/ijms.2024v12i6.52850

Keywords:

Scaevola taccada, Oxidative stress, Gallic acid, Anti-cataract activity

Abstract

Objectives: To evaluate of anti-cataract potential of Scaevola taccada in ex vivo Goat eye models.

Methods: Goat eye lenses separated into five groups were incubated in synthetic aqueous humor culture, added by streptomycin of 0.1 mg/mL was at 37°C for 72 h. Group I use as the normal control. In Group II (disease control), the lenses were added by 1 M glucose. The lenses in Group III (standard control) were incubated in an aqueous humor medium with 1 M glucose added by 500 μg/mL of Gallic acid. The lenses in Group IV (Test Group) were incubated in aqueous humor medium with 1 M glucose added by 500 μg/mL of extract of S. taccada. MDL levels and catalase activity were measured by using ultraviolet Spectroscopy. Statistical analysis of evaluation parameters was conducted using analysis of variance and post hoc Tuckey test.

Results: As a part of the study, in malondialdehyde (MDA) level estimation, our test drug has a low MDA level as compared to the disease control. Hence, it may be acting as an anti-cataract. In total protein estimation, the test drug has a higher amount of total protein in comparison with others. By analyzing catalase activity, Catalase activity data revealed that the test drug is having high catalase activity as compared to the standard control group. Hence, we conclude that it works as an anti-cataract.

Conclusion: The study carried out clearly revealed that S. taccada (Bhadrax) has the potential for anti-cataract activity. This hypothesis was supported by finding such total protein level, MDA level, and catalase activity.

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