118A>G AND IVS2+691G>C POLYMORPHISMS OF OPRM1 GENE HAVE NO INFLUENCE ON COLD-PAIN SENSITIVITY AMONG HEALTHY OPIOID-NAIVE MALAY MALES

Authors

  • Zalina Zahari Department of Pharmacy, Hospital Universiti Sains Malaysia, Kubang Kerian, Kelantan, Malaysia, Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia,
  • Lee Chee Siong Department of Emergency Medicine, School of Medical Sciences, Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia
  • Lee Yeong Yeh School of Medical Sciences, Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia
  • Muslih Abdulkarim Ibrahim Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia5Department of Pharmacology and Toxicology, College of Pharmacy, Hawler Medical University, Hawler, Iraq
  • Nurfadhlina Musa Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia,
  • Md Azhar Mohd Yasin Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia Department of Psychiatry, School of Medical Sciences, Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia
  • Tan Soo Choon
  • Nasir Mohamad Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia, 7Faculty of Medicine & Health Sciences, Universiti Sultan Zainal Abidin, Kuala Terengganu, Terengganu, Malaysia.
  • Rusli Ismail 2Pharmacogenetics and Novel Therapeutics Cluster, Institute for Research in Molecular Medicine (INFORMM), Universiti Sains Malaysia (USM), Kubang Kerian, Kelantan, Malaysia, Centre of Excellence for Research in AIDS (CERiA), University of Malaya, Kuala Lumpur, Malaysia.

Abstract

Objective: Common polymorphisms of the mu-type opioid receptor (OPRM1) including 118A>G and IVS2+691G>C may affect experimental pain responses in healthy subjects, and the effect could be ethnic-dependent. The aim of this study was to investigate the influence of these OPRM1 polymorphisms on cold-pressor pain responses among healthy opioid-naive Malay males.

Methods: Pain-threshold, pain-tolerance, and pain-intensity in response to the cold pressor test (CPT) were measured in healthy opioid-naive Malay males. DNA was extracted from the collected venous blood before PCR-genotyping. Repeated measure analysis of variance (RM-ANOVA) was used to compare CPT responses and OPRM1 polymorphisms (118A>G and IVS2+691G>C) according to their genotypes and allelic additive models, genotype dominant and recessive models, haplotypes, and diplotypes.

Results: A total of 152 participants were recruited. Both 118A>G and IVS2+691G>C polymorphisms were not associated with cold-pressor pain-threshold, pain-tolerance and pain-intensity despite using genotypes and allelic additive models and genotype dominant and recessive models (all p>0.05). Likewise, there were no significant associations between haplotypes and diplotypes for the 118A>G and IVS2+691G>C polymorphisms and the three cold-pain responses (all p>0.05).

Conclusion: The common OPRM1 polymorphisms (i.e., 118A>G and IVS2+691G>C), are not associated with cold-pressor pain responses in healthy opioid-naive Malay males. However, this may be unique for this particular ethnicity. Other polymorphisms may be more relevant for this population, and this should be further investigated.

Keywords: Cold pressor test (CPT), Mu-type opioid receptor (OPRM1), Opioid receptor, mu 1 gene (OPRM1), Pain-threshold, Pain-tolerance, Pain-intensity, Opioid-naive, Male, Malays

Downloads

Download data is not yet available.

References

Mizoguchi H, Narita M, Wu H, Suzuki T, Nagase H, Tseng LF. Differential involvement of mu-opioid receptors in endomorphin-and beta-endorphin-induced G-protein activation in the mouse pons/medulla. Neuroscience 2000; 100:835-9.

Feng Y, He X, Yang Y, Chao D, Lazarus LH, Xia Y. Current research on opioid receptor function. Curr Drug Targets 2012;13:230-46.

Saidak Z, Blake-Palmer K, Hay DL, Northup JK, Glass M. Differential activation of G-proteins by mu-opioid receptor agonists. Br J Pharmacol 2006;147:671-80.

Zubieta JK, Smith YR, Bueller JA, Xu Y, Kilbourn MR, Jewett DM, et al. Regional mu opioid receptor regulation of sensory and affective dimensions of pain. Science 2001;293:311-5.

Young EE, Lariviere WR, Belfer I. Genetic basis of pain variability: recent advances. J Med Genet 2012;49:1-9.

Govoni S, Regazzi M, Ranzani GN. Pain and the pharmacogenetics at the fuzzy border between pain physiopathology and pain treatment. Eur J Pain Suppl 2008;2 Suppl 1:5-12.

Lind AL, Gordh TE. Research on genes predisposing for chronic pain: a challenge for pain researchers in scandinavia. Scandinavian J Pain 2009;1 Suppl 1:24-6.

Lötsch J, Geisslinger G, Tegeder I. Genetic modulation of the pharmacological treatment of pain. Pharmacol Ther 2009;124:168-84.

Miaskowski C. Understanding the genetic determinants of pain and pain management. Semin Oncol Nurs 2009;25(2, Suppl 1):1-7.

Kim DH, Schwartz CE. The genetics of pain: implications for evaluation and treatment of spinal disease. Spine J 2010;10:827-40.

Kasai S, Ikeda K. Pharmacogenomics of the human μ-opioid receptor. Pharmacogenomics 2011;12:1305-20.

Shipton EA. Pharmacogenomics in acute pain. Trends Anaesthesia Critical Care 2011;1:117-22.

Hagelberg N, Aalto S, Tuominen L, Pesonen U, Nagren K, Hietala J, et al. Striatal mu-opioid receptor availability predicts cold pressor pain-threshold in healthy human subjects. Neurosci Lett 2012;521:11-4.

Fillingim RB, Kaplan L, Staud R, Ness TJ, Glover TL, Campbell CM, et al. The A118G single nucleotide polymorphism of the mu-opioid receptor gene (OPRM1) is associated with pressure pain sensitivity in humans. J Pain 2005;6:159-67.

Lotsch J, Stuck B, Hummel T. The human μ-opioid receptor gene polymorphism 118A>G decreases cortical activation in response to specific nociceptive stimulation. Behav Neurosci 2006;120:1218-24.

Hastie BA, Riley JL, Kaplan L, Herrera DG, Campbell CM, Virtusio K, et al. Ethnicity interacts with the OPRM1 gene in experimental pain sensitivity. Pain 2012;153:1610-9.

Huang CJ, Liu HF, Su NY, Hsu YW, Yang CH, Chen CC, et al. Association between human opioid receptor genes polymorphisms and pressure pain sensitivity in females*. Anaesthesia 2008;63:1288-95.

Chen AC, Dworkin SF, Haug J, Gehrig J. Human pain responsivity in a tonic pain model: psychological determinants. Pain 1989;37:143-60.

Compton P, Charuvastra VC, Ling W. Pain intolerance in opioid-maintained former opiate addicts: effect of long-acting maintenance agent. Drug Alcohol Depend 2001;63:139-46.

Zahari Z, Lee CS, Tan SC, Mohamad N, Lee YY, Ismail R. Relationship between cold pressor pain-sensitivity and sleep quality in opioid-dependent males on methadone treatment. Peer J 2015;3:e839.

Wolf S, Hardy JD. Studies on pain. Observations on pain due to local cooling and on factors involved in the cold pressor†effect. J Clin Invest 1941;20:521-33.

Harris G, Rollman GB. The validity of experimental pain measures. Pain 1983;17:369-76.

Glynn CJ, Lloyd JW. The diurnal variation in perception of pain. Proc R Soc Med 1976;69:369-72.

Mohamed Nazar NI. Therapeutic drug monitoring in methadone maintenance therapy (mmt): an evaluation of genetic factors influencing clinical outcomes and serum concentrations of methadone: Ph. D Thesis, Universiti Sains Malaysia; 2013.

Cohen J. A power primer. Psychol Bull 1992;112:155-9.

Belfer I, Segall SK, Lariviere WR, Smith SB, Dai F, Slade GG, et al. Pain modality-and sex-specific effects of COMT genetic functional variants. Pain 2013;154:1368-76.

Tan EC, Tan CH, Karupathivan U, Yap EP. Mu opioid receptor gene polymorphisms and heroin dependence in Asian populations. Neuroreport 2003;14:569-72.

Zhang W, Chang YZ, Kan QC, Zhang LR, Lu H, Chu QJ, et al. Association of human μ-opioid receptor gene polymorphism A118G with fentanyl analgesia consumption in Chinese gynaecological patients. Anaesthesia 2010;65:130-5.

Fukuda K, Hayashida M, Ide S, Saita N, Kokita Y, Kasai S, et al. Association between OPRM1 gene polymorphisms and fentanyl sensitivity in patients undergoing painful cosmetic surgery. Pain 2009;147:194-201.

Tan EC, Lim EC, Teo YY, Lim Y, Law HY, Sia AT. Ethnicity and OPRM variant independently predict pain perception and patient-controlled analgesia usage for post-operative pain. Mol Pain 2009;5:32.

Bond C, LaForge KS, Tian M, Melia D, Zhang S, Borg L, et al. Single-nucleotide polymorphism in the human mu opioid receptor gene alters beta-endorphin binding and activity: possible implications for opiate addiction. Proc Natl Acad Sci U S A 1998;95:9608-13.

Beyer A, Koch T, Schroder H, Schulz S, Hollt V. Effect of the A118G polymorphism on binding affinity, potency and agonist-mediated endocytosis, desensitization, and resensitization of the human mu-opioid receptor. J Neurochem 2004;89:553-60.

Zhang Y, Wang D, Johnson AD, Papp AC, Sadee W. Allelic expression imbalance of human mu opioid receptor (OPRM1) caused by variant A118G. J Biol Chem 2005;280:32618-24.

Muralidharan A, Smith MT. Pain, analgesia and genetics. J Pharm Pharmacol 2011;63:1387-400.

Fillingim RB, Maixner W. Gender differences in the responses to noxious stimuli. Pain Forum 1995;4:209-21.

Alabas OA, Tashani OA, Tabasam G, Johnson MI. Gender role affects experimental pain responses: a systematic review with meta-analysis. Eur J Pain 2012;16:1211-23.

Fillingim RB, King CD, Ribeiro-Dasilva MC, Rahim-Williams B, Riley JL. Sex, gender, and pain: a review of recent clinical and experimental findings. J Pain 2009;10:447-85.

Racine M, Tousignant-Laflamme Y, Kloda LA, Dion D, Dupuis G, Choiniere M. A systematic literature review of 10 y of research on sex/gender and experimental pain perception-part 1:are there really differences between women and men? Pain 2012;153:602-18.

Angst MS, Phillips NG, Drover DR, Tingle M, Ray A, Swan GE, et al. Pain sensitivity and opioid analgesia: a pharmacogenomic twin study. Pain 2012;153:1397-409.

Shavers VL, Bakos A, Sheppard VB. Race, ethnicity, and pain among the U. S. adult population. J Health Care Poor Underserved 2010;21:177-220.

Edwards CL, Fillingim RB, Keefe F. Race, ethnicity and pain. Pain 2001;94:133-7.

Carter LE, McNeil DW, Vowles KE, Sorrell JT, Turk CL, Ries BJ, et al. Effects of emotion on pain reports, tolerance and physiology. Pain Res Manag 2002;7:21-30.

Published

01-07-2016

How to Cite

Zahari, Z., L. C. Siong, L. Y. Yeh, M. A. Ibrahim, N. Musa, M. A. M. Yasin, T. S. Choon, N. Mohamad, and R. Ismail. “118A>G AND IVS2+691G>C POLYMORPHISMS OF OPRM1 GENE HAVE NO INFLUENCE ON COLD-PAIN SENSITIVITY AMONG HEALTHY OPIOID-NAIVE MALAY MALES”. International Journal of Pharmacy and Pharmaceutical Sciences, vol. 8, no. 7, July 2016, pp. 73-80, https://journals.innovareacademics.in/index.php/ijpps/article/view/10423.

Issue

Section

Original Article(s)